Advanced green biorefining: effects of ensiling treatments on lactic acid production, microbial activity and supplementary methane formation of grass and rye

Haag, Nicola Leonard; Grumaz, Christian; Wiese, Franziska; Kirstahler, Philipp; Merkle, Wolfgang; Nägele, Hans‐Joachim; Sohn, Kai; Jungbluth, Thomas; Oechsner, Hans

doi:10.1007/s13399-015-0178-2

Cited by 14 publications

(14 citation statements)

References 20 publications

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“…These include, for example, proteins that can be extracted for use in other areas of food production (see Santamaría-Fernandez, Molinuevo-Salces, Lübeck, & Uellendahl, 2017). Furthermore, various acids such as lactic acids can be decoupled, which can be used for biopolymer production (e.g., Haag et al, 2016). However, various fatty acids also offer potential for platform chemicals.…”

Section: Productsmentioning

confidence: 99%

Status quo and perspectives of biogas production for energy and material utilization

Bahrs

Angenendt

2018

GCB Bioenergy

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Biogas is in many respects a serious alternative to other fossil resources and complements other renewable energy sources from wind and sun. Biogas can be produced in many places decentrally. Its energy potential is high, and it is widely used in the EU and all over the world. With more than 16,000 ktoe of oil equivalent in the EU in 2016, it corresponds to approximately 8% of the total primary energy produced by renewable energies in the EU, produced with nearly 17,000 biogas plants. Nevertheless, the production costs of biogas and its products like energy, heat, and fuel are still too high. Kost et al. (2018) show a comparison of electricity generation costs of different renewable energies and their future potentials. While electricity from huge biogas plants offers generation costs from 10 to 15 ct/kWh, electricity from onshore wind and huge solar systems offers generation costs from 4 to 8 ct/kWh. Although substantial progress has been made with regard to substrate use, production techniques and market designs, many more innovations are needed throughout the biogas value chain for it to be competitive in energy markets without high subsidies. As several papers in the special issue on biogas show, there are numerous innovations and product designs with regard to energy and material uses that could maintain or even increase the importance of biogas production both within and outside of the EU. There are many potential benefits of biogas, as it offers high shares of produced renewable energies as well as large amounts of material products like digestates and in future maybe products of higher value such as proteins or lactic acids.

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Section: Productsmentioning

confidence: 99%

Status quo and perspectives of biogas production for energy and material utilization

Bahrs

Angenendt

2018

GCB Bioenergy

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“…Lactic acid (LA) is a versatile chemical and has been widely applied in various industries including food, leather tanning, cosmetics, and pharmaceuticals (Martinez et al 2013). Approximately 90% of global LA is produced via microbial fermentation (Haag et al 2016;Alexandri et al 2019), which requires low production temperature and has low energy requirements and high purity (Wee et al 2006). Ensiling is an anaerobic fermentation process, which naturally depends on the epiphytic lactic acid bacteria (LAB) fermenting water-soluble carbohydrates (WSC) into LA to decrease pH.…”

Section: Introductionmentioning

confidence: 99%

“…Consequently, LA-dominated plants can be well conserved as feedstock (McDonald 1981). In recent years, ensiling has been de ned as an eco-friendly, green, and sustainable lignocellulose degradation technique for producing LA from biomass (Haag et al 2016;Bichot et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Clarifying Key Cellulase Component Cooperated With Lactic Acid Bacteria for Alfalfa Lignocellulose Degradation to Improve Lactic Acid Production

Ceng

et al. 2021

Preprint

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Clarifying key cellulase component that played synergistic roles with lactic acid bacteria (LAB) in fermenting alfalfa lignocellulose into lactic acid (LA) is valuable in low-temperature seasons. Last cut and low dry matter (DM) alfalfa was ensiled by 9 treatments, combinations of cellulase component genes engineered Lactoc. lactis subsp. lactis MG1363 strains (HT2, HT3, HT4, HT5, E1C1, E1B1, and C1B1, separately containing bgl1, cbh2, and egl3 gene were mixed at 1:1:1, 2:1:1, 1:2:1, 1:1:2, 1:1:0, 1:0:1, and 0:1:1), cellulase (EN), and a combination of Lactobacillus plantarum and cellulase (LPEN), and without treatments, as the control, with 4 replicates each. After anaerobic preservation in a silo from late fall through winter (3-20℃) for 140 d, the ensiled alfalfa was sampled and analysed. EN degraded lignocellulose best but the pH was the key limiting factor for lignocellulose saccharification of commercial EN in the simultaneous saccharification and fermentation of LPEN. The optimal combination HT4 caused the fewest disaccharide (1.02 g/kg DM) and the highest conversion of water-soluble carbohydrates (WSC) to LA (170%) and increased LA content to 80.0 g/kg DM maximally since cellobiohydrolase better cooperated with Lactoc. lactis host to ferment lignocellulose into LA than endoglucanase and β-glucosidase. Therefore, strong LA production was approached in HT4 by clarifying key cellulase component played synergistic roles with Lactoc. lactis host. This study could benefit the development of LA production in fermenting lignocellulosic biomass.

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“…Furthermore, the role of storage possibilities and the full-year delivery of the biomass is discussed in the literature [ 50 ]. A well-known method for full-year storage and delivery is ensiling of the substrate [ 51 , 52 , 53 ], especially for maize [ 54 ], corn stover [ 55 ], or LCB [ 56 ].…”

Section: Introductionmentioning

confidence: 99%

Maize Silage Pretreatment via Steam Refining and Subsequent Enzymatic Hydrolysis for the Production of Fermentable Carbohydrates

Krafft¹,

Frey²,

Schwarz³

et al. 2020

Molecules

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Maize, also called corn, is one of the most available feedstocks worldwide for lignocellulosic biorefineries. However, a permanent biomass supply over the year is essential for industrial biorefinery application. In that context, ensiling is a well-known agricultural application to produce durable animal feed for the whole year. In this study, ensiled maize was used for steam refining experiments with subsequent enzymatic hydrolysis using the Cellic® CTec2 to test the application possibilities of an ensiled material for the biorefinery purpose of fermentable carbohydrate production. Steam refining was conducted from mild (log R0 = 1.59) to severe conditions (log R0 = 4.12). The yields were determined, and the resulting fractions were characterized. Hereafter, enzymatic hydrolysis of the solid fiber fraction was conducted, and the carbohydrate recovery was calculated. A conversion to monomers of around 50% was found for the mildest pretreatment (log R0 = 1.59). After pretreatment at the highest severity of 4.12, it was possible to achieve a conversion of 100% of the theoretical available carbohydrates. From these results, it is clear that a sufficient pretreatment is necessary to achieve sufficient recovery rates. Thus, it can be concluded that ensiled maize pretreated by steam refining is a suitable and highly available feedstock for lignocellulosic biorefineries. Ultimately, it can be assumed that ensiling is a promising storage method to pave the way for a full-year biomass supply for lignocellulosic biorefinery concepts.

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Advanced green biorefining: effects of ensiling treatments on lactic acid production, microbial activity and supplementary methane formation of grass and rye

Cited by 14 publications

References 20 publications

Status quo and perspectives of biogas production for energy and material utilization

Status quo and perspectives of biogas production for energy and material utilization

Clarifying Key Cellulase Component Cooperated With Lactic Acid Bacteria for Alfalfa Lignocellulose Degradation to Improve Lactic Acid Production

Maize Silage Pretreatment via Steam Refining and Subsequent Enzymatic Hydrolysis for the Production of Fermentable Carbohydrates

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