Advanced Oxidation Protein Products and Carbonylated Proteins as Biomarkers of Oxidative Stress in Selected Atherosclerosis-Mediated Diseases

Gryszczyńska, Bogna; Formanowicz, Dorota; Budzyń, Magdalena; Wanic-Kossowska, Maria; Pawliczak, Elżbieta; Formanowicz, Piotr; Majewski, Wacław; Strzyżewski, Krzysztof; Kasprzak, Magdalena Paulina; Iskra, Maria

doi:10.1155/2017/4975264

Cited by 62 publications

(55 citation statements)

References 38 publications

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“…AOPP can be considered as protein oxidation markers generated by the reaction between proteins and chlorinated oxidants derived mainly from myeloperoxidase by activated neutrophils. 12,16,17 In this context, it can be speculated that the pasteurization process is an important step in the process of preserving the protein and lipid integrity of breast milk, due to the inactivation of polymorphonuclear neutrophil leukocytes present in it. Regarding the effects of pasteurization on the biological components of human milk, a possible explanation for the significant variability in the data reported in the scientific literature is the heterogeneity of the test and study protocols (for example, in terms of sample origin, storage conditions or methods of analysis).…”

Section: Discussionmentioning

confidence: 99%

“…[11][12][13][14][15][16] Protein oxidation products (AOPP) have also been understood as new markers of oxidation and damage to proteins and can be used to estimate protein oxidative damage. [12][13][14][15][16][17] Malondialdehyde (MDA) is one of the final products of lipid peroxidation (LP) and, being a stable product, it can be used as a cumulative measure of this process. 16 Therefore, this study aimed to evaluate the antioxidant and oxidant profile of fresh human milk and after the pasteurization process.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Pasteurization on the Antioxidant and Oxidant Properties of Human Milk

Lorençoni

Silva

Júnior

2021

Rev. paul. pediatr.

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Objective: To evaluate the effect of pasteurization on antioxidant and oxidant properties of human milk. Methods: 42 samples of milk before and after pasteurisation were used to evaluate the antioxidant activity by the ferric reducing capacity and by scavenging the 2,2’-azino-bis 3-ethylbenzthiazoline-6-sulfonic acid radical. Lipid peroxidation was estimated by the concentration of malondialdehyde product using the thiobarbituric acid reactive substances assay and by the evaluation of advanced oxidation protein products. Results: No significant difference was observed in fresh human milk and after pasteurization in relation to antioxidant properties determined by the ferric reducing capacity (50.0±3.4% and 48.8±3.0%, respectively) and by scavenging the 2,2’-azino-bis 3-ethylbenzthiazoline-6-sulfonic acid radical (28.9±1.5% and 31.2±1.3%, respectively). The results of malondialdehyde (62.6±4.1 and 64.3±3.6 µM/mg) and protein oxidation products (59.4±3.4 and 54.2±3.8 µM/L) of fresh and pasteurized milk, respectively, did not exhibited any significant difference. Conclusions: This data showed that human milk has an important antioxidant activity and that the pasteurizing process does not influence the antioxidant capacity, avoiding the peroxidation of breast milk lipids and the formation of advanced protein oxidation products.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of Pasteurization on the Antioxidant and Oxidant Properties of Human Milk

Lorençoni

Silva

Júnior

2021

Rev. paul. pediatr.

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“…Samples were diluted 1: 6 and assayed in duplicate. AOPP results from the interaction of reactive oxygen species with proteins to yield modifications including dityrosine, pentosidine, and carbonyls [53]. Increased AOPP levels are associated with many pro-inflammatory diseases and are used as a biomarker for oxidative stress [54].…”

Section: Plasma Measurementsmentioning

confidence: 99%

Chronic Testosterone Deprivation Sensitizes the Middle-Aged Rat Brain to Damaging Effects of Testosterone Replacement

et al. 2019

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Introduction: An increasing number of middle-aged men are being screened for low testosterone levels and the number of prescriptions for various forms of testosterone replacement therapy (TRT) has increased dramatically over the last 10 years. However, the safety of TRT has come into question with some studies suggesting increased morbidity and mortality. Objective: Because the benefits of estrogen replacement in postmenopausal women and ovariectomized rodents are lost if there is an extended delay between estrogen loss and replacement, we hypothesized that TRT may also be sensitive to delayed replacement. Methods: We compared the effects of testosterone replacement after shortterm (2 weeks) and long-term testosterone deprivation (LTTD; 10 weeks) in middle-aged male rats on cerebral ischemia, oxidative stress, and cognitive function. We hypothesized that LTTD would increase oxidative stress levels and abrogate the beneficial effects of TRT. Results: Hypogonadism itself and TRT after short-term castration did not affect stroke outcome compared to intact rats. However, after long-term hypogonadism in middle-aged male Fischer 344 rats, TRT exacerbated the detrimental behavioral effects of experimental focal cerebral ischemia, whereas this detrimental effect was prevented by administration of the freeradical scavenger tempol, suggesting that TRT exacerbates oxidative stress. In contrast, TRT improved cognitive performance in non-stroked rats regardless of the length of hypogonadism. In the Morris water maze, peripheral oxidative stress was highly associated with decreased cognitive ability. Conclusions: Taken together, these data suggest that TRT after long-term hypogonadism can exacerbate functional recovery after focal cerebral ischemia, but in the absence of injury can enhance cognition. Both of these effects are modulated by oxidative stress levels.

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“…Carbonyl derivatives can appear in different ways: As a result of direct oxidation of the amino acid residues of lysine, arginine, proline and threonine, or under the action of reactive oxygen species, as well as in reactions with carbonyl-containing oxidized lipids. Other carbonylation pathways include protein fragmentation, α-amidation, and oxidation of glutamine side chains [26]. Protein carbonylation is an irreversible process leading to a loss of proteins' functions.…”

Section: Introductionmentioning

confidence: 99%

The Level of Reactive Carbonyl Derivatives of Proteins, Methylglyoxal, and Malondialdehyde in Rats Experiencing Chronic Unpredictable Moderate Stress

Yepifantseva¹,

Muravlyova²,

Semenikhina³

et al. 2020

Open Access Maced J Med Sci

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BACKGROUND: Prolonged exposure to stress is known to change homeostasis and can lead to the development of psychopathological disorders, including depression. Exposure to stress factors contributes to the emergence of reactive oxygen species in the brain. The data characterizing changes of the oxidative metabolism under conditions of chronic stress are insufficient, which determine the relevance of the research in this direction. The determination of reactive carbonyl derivatives of proteins (RCDP), methylglyoxal (MG), and malondialdehyde (MDA) levels can be considered an informative method for studying the dynamics of oxidative metabolism. AIM: The aim of the study was to study the RCDP, MG, and MDA in red blood cells and blood plasma in rats experiencing chronic unpredictable moderate stress (CUMS). METHODS: A total of 20 male outbred rats weighing 450–500 g were used in the study. The animals were divided into two groups of ten rats each using randomized selection. The experimental group of animals was exposed to the impact of diverse stress factors, according to the CUMS model for 21 days. The control group of animals remained in standard conditions and did not undergo CUMS model. Blood sampling was performed twice for all rats the day before the experiment started and on 21st day to determine the level of RCDP, MG, and MDA in their erythrocytes and blood plasma. RESULTS: The study revealed a significant decrease of RCDP (p = 0.007) and MDA levels in erythrocytes (p = 0.013), and MDA level in blood plasma (p = 0.005) of rats of the main experimental group. The control group showed a significant increase of RCDP level in plasma (p = 0.008), a two-fold increase of MDA in erythrocytes (p = 0.05) with a decrease of MDA in plasma (p = 0.011). A tendency for MG increase was noted in erythrocytes of rats in both the main group and the control group. CONCLUSION: The early stages of CUMS were accompanied by the violation of oxidative metabolism with the emergence and accumulation of RCDP and MDA in the blood of animals. The decreased emergence of RCDP and MDA in the later stages can be associated with an excess of oxidized substrates in the blood of animals of the experimental group. The revealed trend toward MG growth in animals of both groups suggests its increased synthesis in erythrocytes, which, in our opinion, should be considered a negative factor affecting the deformability of red cells. In turn, a violation of the deformability of red blood cells leads to a violation of microcapillary blood flow, which contributes to the development of hypoxic damage in different tissues. Thus, the obtained data confirm the different directions of changes in RCDP and MDA levels in the blood of animals experiencing CUMS or acute stress. Our results prove the need for further research on changes in oxidative metabolism in chronic stress disorders.

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Advanced Oxidation Protein Products and Carbonylated Proteins as Biomarkers of Oxidative Stress in Selected Atherosclerosis-Mediated Diseases

Cited by 62 publications

References 38 publications

Effect of Pasteurization on the Antioxidant and Oxidant Properties of Human Milk

Effect of Pasteurization on the Antioxidant and Oxidant Properties of Human Milk

Chronic Testosterone Deprivation Sensitizes the Middle-Aged Rat Brain to Damaging Effects of Testosterone Replacement

The Level of Reactive Carbonyl Derivatives of Proteins, Methylglyoxal, and Malondialdehyde in Rats Experiencing Chronic Unpredictable Moderate Stress

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