Advanced Oxidation Protein Products and Carbonylated Proteins Levels in Endovascular and Open Repair of an Abdominal Aortic Aneurysm: The Effect of Pre-, Intra-, and Postoperative Treatment

Abstract

Background. In recent years, a rapid increase in studies focusing on the role of oxidative stress in the pathogenesis of an abdominal aortic aneurysm (AAA) has been observed. Oxidative modifications of proteins are infrequently evaluated in reference to AAA. Objectives. The intensity of oxidative protein modifications, presented as advanced oxidation protein products (AOPP) and carbonylated proteins (C=O), in AAA patients qualified for surgery was estimated. The effect of surgical techniques and intraoperative and postoperative treatment on AOPP and C=O levels was evaluated. Patients. The EVAR group, consisting of 30 patients, was classified for endovascular aneurysm repair, whereas 28 patients were classified for conventional open repair (OR). Methods. AOPP and C=O were measured using a colorimetric assay kit. Results. A significantly lower AOPP level obtained 2-4 days after EVAR surgery in comparison with the value found before surgery was noted. In the case of OR postoperative treatment, a tendency of AOPP level to increase was observed. The tendency of C=O to decrease after surgery in the EVAR group was indicated. However, the C=O level tended to increase after OR surgery and reached a significantly higher value 5-7 days after surgery compared with the value obtained before surgery. Conclusions. Based on our results, it may be concluded that AAA as well as surgical technique contribute to the formation of AOPP and C=O. The analysis of changes in AOPP and C=O values obtained after surgery revealed a significant effect of a patient’s condition before surgery as well as the choice of surgery technique on the values of the studied parameters revealed during postoperative treatment.

Loganic Acid, an Iridoid Glycoside Extracted from Cornus mas L. Fruits, Reduces of Carbonyl/Oxidative Stress Biomarkers in Plasma and Restores Antioxidant Balance in Leukocytes of Rats with Streptozotocin-Induced Diabetes Mellitus

“…Formation of OMP characterize by introduction of additional carbonyl groups into proteins. Carbonylation of proteins may be due to oxidation of side chains of some amino acid such as methionine, lysine and arginine or their interaction with ROS and non-oxidative reactions with oxidized lipids containing carbonyl groups [ 39 ]. Instead, we have also investigated the level of AOPPs in plasma and leukocytes, which are formed as a result of the reaction between chlorinated oxidants and proteins.…”

Common and Novel Markers for Measuring Inflammation and Oxidative Stress Ex Vivo in Research and Clinical Practice—Which to Use Regarding Disease Outcomes?

“…In an RCT with T2D individuals, administering carnosine as an antioxidant significantly reduced PCs vs. placebo [ 181 ]. PCs have also been shown to increase within 5–7 days post-operation, thus AOPPs and PCs may be observed after some time-lag of the original stimulus [ 245 ]. Thus, though there is less data compared to other OS markers, PCs constitute an interesting choice with clinical relevance, though the diversity of analytical methods impedes comparisons across studies.…”

Reduced Levels of Selenium and Thioredoxin Reductase in the Thoracic Aorta Could Contribute to Aneurysm Formation in Patients with Marfan Syndrome

“…This oxidative background can be reflected in the increased carbonylation in the TAA. In this sense, carbonylation is a marker of damage to amino acids such as lysine, methionine, and arginine, and also indicates the loss of disulfide bridges between the cysteines on the primary structure of the proteins by ROS [ 37 ]. At the same time, this contributes to endothelial dysfunction in the TAA.…”