“…The Bionanoprobe (BNP) at beamline 9-ID of the Advanced Photon Source (APS) at Argonne National Laboratory focuses on advancing synchrotron-based X-ray fluorescence (XRF) imaging and elemental mapping techniques for a broad range of biological studies including the imaging of mouse fibroblast cells (Chen et al, 2015), the effect of nanomedicine on rabbit liver tissues via sulfur maps (Deng et al, 2022), a simultaneous qualitative investigation of structural features and quantitative elemental maps of ex vivo tissues (Genoud et al, 2020), and specifying discrete zinc-enriched vesicles in oocyte growth and egg fertilization via single-cell level zinc mapping (Que et al, 2015). Facilitation of further scientific discovery at the 9-ID beamline as well as other beamlines at the APS and their consequent scientific impact is expected to accelerate with the upcoming APS upgrade (APS-U) providing higher-energy X-rays for faster measurement and higher resolution (Fornek, 2019). The orders-of-magnitude increase in acquired XRF images necessitates integration of machine-learning methods for data analysis, smart experi-mentation, and autonomous decision-making for interpretation and exploration of high-dimensional parameter spaces.…”