Advanced platelet‐rich fibrin and freeze‐dried bone allograft for ridge preservation: A randomized controlled clinical trial

Clark, Daniel; Rajendran, Yogalakshmi; Paydar, Sarmad; Ho, Sunita P.; Cox, Darcy; Ryder, Mark I.; Dollard, John; Kao, Richard T.

doi:10.1002/jper.17-0466

Cited by 106 publications

(159 citation statements)

References 29 publications

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“…This research was inspired by the clinical observation that PRF supports alveolar ridge preservation, suggesting a possible impact of PRF to suppress the formation of bone‐resorbing osteoclasts . Therefore, we induced osteoclastogenesis in vitro using murine bone marrow in the presence of soluble extracts from PRF membranes.…”

Section: Discussionmentioning

confidence: 99%

“…The clinical relevance remains a matter of speculation because it is not clear whether the preservation of the alveolar ridge is a consequence of decreased osteoclastogenesis and bone resorption, a compensatory activation of bone‐forming osteoblasts, or both. On the other hand, based on the present findings, PRF cannot reverse the process once osteoclasts have developed.…”

Section: Discussionmentioning

confidence: 99%

“…Platelet‐rich fibrin (PRF), an autologous preparation of coagulated plasma, was originally introduced to improve wound healing . Today, however, emerging evidence from randomized controlled trials suggests that PRF can preserve horizontal and vertical ridge dimension after tooth extraction . Further, preclinical studies support the use of PRF in bone regeneration.…”

Section: Introductionmentioning

confidence: 99%

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Platelet‐rich fibrin suppresses in vitro osteoclastogenesis

Kargarpour

Nasirzade

Strauß

et al. 2019

Journal of Periodontology

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Background Platelet‐rich fibrin (PRF) membranes can preserve alveolar ridge dimension after tooth extraction. Thus, it can be presumed that PRF suppresses the catabolic events that are caused by osteoclastic bone resorption. Methods To address this possibility, we investigated the impact of soluble extracts of PRF membranes on in vitro osteoclastogenesis in murine bone marrow cultures. Osteoclastogenesis was induced by exposing murine bone marrow cultures to receptor activator of nuclear factor kappa B ligand (RANKL), macrophage colony‐stimulating factor (M‐CSF) and transforming growth factor‐beta 1 (TGF‐β1) in the presence or absence of PRF. Osteoclastogenesis was evaluated based on histochemical, gene expression, and resorption analysis. Viability was confirmed by formation of formazan crystals, live‐dead staining and caspase‐3 activity assay. Results We report here that in vitro osteoclastogenesis is greatly suppressed by soluble extracts of PRF membranes as indicated by tartrate‐resistant acid phosphatase (TRAP) staining and pit formation. In support of the histochemical observations, soluble extracts of PRF membranes decreased expression levels of the osteoclast marker genes TRAP, Cathepsin K, dendritic cell‐specific transmembrane protein (DCSTAMP), nuclear factor of activated T‐cells (NFATc1), and osteoclast‐associated receptor (OSCAR). PRF membranes, however, cannot reverse the process once osteoclastogenesis has evolved. Conclusion These in vitro findings indicate that PRF membranes can inhibit the formation of osteoclasts from hematopoietic progenitors in bone marrow cultures. Overall, our results imply that the favorable effects of PRF membranes in alveolar ridge preservation may be attributed, at least in part, by the inhibition of osteoclastogenesis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Platelet‐rich fibrin suppresses in vitro osteoclastogenesis

Kargarpour

Nasirzade

Strauß

et al. 2019

Journal of Periodontology

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“…In split‐mouth studies, PRF promoted better soft tissue healing and a faster bone regeneration in comparison to PRP (Doiphode, Hegde, Mahindra, Santhosh Kumar & Tenglikar, ; Yelamali & Saikrishna, ). On the contrary, a recent study showed no significant differences in terms of bone density and alveolar ridge dimensions for sockets treated with A‐PRF, FDBA or a combination of A‐PRF and FDBA (Clark, Rajendran, Paydar, Ho & Cox, ). Adding PRF to Plaster of Paris also did not return any significant benefit for ridge preservation (Girish Kumar, Chaudhary, Kumar, Arora & Kumar, ).…”

Section: Resultsmentioning

confidence: 90%

The use of bioactive factors to enhance bone regeneration: A narrative review

Donos

Dereka

Calciolari

2019

J Clinic Periodontology

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Aim This review critically appraises the available knowledge on the pre‐clinical and clinical use of bioactive factors for bone regeneration in the cranial and maxillofacial area. Materials and Methods The use of growth factors, amelogenins and autologous platelet concentrates (APCs) for bone regeneration was reviewed in a systematic manner. More specifically, pre‐clinical and clinical studies on ridge preservation, alveolar ridge augmentation, regeneration of peri‐implant defects and sinus augmentation models were considered. Results Amongst different bioactive factors, the highest pre‐clinical and clinical evidence of a positive effect on bone formation is associated with rhBMP‐2 and the lowest with amelogenins. While APCs seem to accelerate clinical healing and reduce postoperative discomfort, there is insufficient and contrasting evidence of a significant effect on hard tissue regeneration for the different clinical applications. Conclusions Although there is increasing evidence that bioactive factors might enhance the bone regeneration process, the great heterogeneity of the available studies and the limited number of RCTs do not allow to draw robust conclusions. Issues that still need to be investigated include the optimal carriers for bioactive agents (direct vs. indirect), the dosage, the timing of administration, as well as the possibility of combining different agents to promote synergistic effects.

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“…Advanced plateletrich fibrin (A-PRF) is one such product and recently being used in alveolar ridge preservation procedures. Clark et al 185 conducted a multiarm parallel randomized controlled clinical trial to evaluate the efficacy of A-PRF alone or with freeze-dried bone allograft (FDBA) in improving vital bone formation and alveolar dimensional stability. Forty patients requiring extraction of nonmolar teeth and replacement with dental implants were randomized into 1 of 4 ridge preservation approaches: A-PRF, A-PRF+FDBA, FDBA, or blood clot.…”

Section: Alveolar Ridge Preservation Ridge and Sinus Augmentationmentioning

confidence: 99%