2005
DOI: 10.1016/j.bbrc.2005.09.137
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Advanced procedures for separation and analysis of low molecular weight inhibitor (NCXIF) of the cardiac sodium–calcium exchanger

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Cited by 8 publications
(21 citation statements)
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“…What is known is that NCX IF is a small, polar, and uncharged compound that is poorly retained even on polar-embedded RP columns. HILIC on an amide silica column was used alongside gel filtration, cation exchange, and C 30 RP separation a few years ago [151], and recently the same authors revisited the problem [152] with a set of new columns of which two were HILIC phases (poly(succinimide) and poly(2-hydroxyethyl aspartamide) silica) and one a b-cyclodextran silica operated in NP mode in their continued efforts to purify the elusive substance for identification. The HILIC and NP separations were found imperative for the overall purification scheme, but provided a much lower yield of the NCX IF activity (30 -60%) compared to the RP columns.…”
Section: Pharmaceutical Development and Drug Discoverymentioning
confidence: 99%
“…What is known is that NCX IF is a small, polar, and uncharged compound that is poorly retained even on polar-embedded RP columns. HILIC on an amide silica column was used alongside gel filtration, cation exchange, and C 30 RP separation a few years ago [151], and recently the same authors revisited the problem [152] with a set of new columns of which two were HILIC phases (poly(succinimide) and poly(2-hydroxyethyl aspartamide) silica) and one a b-cyclodextran silica operated in NP mode in their continued efforts to purify the elusive substance for identification. The HILIC and NP separations were found imperative for the overall purification scheme, but provided a much lower yield of the NCX IF activity (30 -60%) compared to the RP columns.…”
Section: Pharmaceutical Development and Drug Discoverymentioning
confidence: 99%
“…An advanced procedure for the separation and analysis of a low molecular weight cardiac sodium/calcium exchanger inhibitor (NCX IF ) was reported by Khananshvili and coworkers [111]. Three chromatographic modes were studied including RPLC, NPLC, and HILIC in combination with ESI-MS. For RPLC, the retention time of NCX IF on the C18 column did not increase when ion-pairing reagents like TFA or heptafluorobutyric acid (HFBA) were added to the mobile phase.…”
Section: Drug Discoverymentioning
confidence: 99%
“…In addition, it is convenient to hyphenate HILIC with various detectors [1 -5], especially mass spectrometers (MS) [6]. These advantages make HILIC increasingly applied in the qualitative and quantitative analysis of target compounds in pharmaceutical [7 -9], medical [10,11], toxic [12], biochemical [13], and phytochemistry studies [14 -17] recently. Some relevant reviews on the development of HILIC [18] and its application in the separation of peptides [19], pharmaceuticals [20], and bioanalysis [21] were published as well.…”
Section: Introductionmentioning
confidence: 99%