Advanced separation methods of food anthocyanins, isoflavones and flavanols

Valls, Josep; Millán, Silvia; Marti, M. P. Villanua; Borràs, Eva; Arola, Lluı́s

doi:10.1016/j.chroma.2009.07.030

Cited by 266 publications

(188 citation statements)

References 195 publications

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“…Unlike reverse-phase, normal-phase HPLC uses an organic mobile phase and rather than separating specific PC compounds, it elutes PCs of the same DP 110,112 . While this is an efficient method, it is unfavorable with highly polymerized samples.…”

mentioning

confidence: 99%

Oligomeric Cocoa Procyanidins Possess Enhanced Bioactivity Compared to Monomeric and Polymeric Cocoa Procyanidins for Preventing the Development of Obesity, Insulin Resistance, and Impaired Glucose Tolerance during High-Fat Feeding

Dorenkott

Griffin

Goodrich

et al. 2014

J. Agric. Food Chem.

134

102

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Cocoa is a major source of these flavanols. However, research on the potential anti-obesity and anti-diabetic health benefits of cocoa flavanols is lacking in the literature. Furthermore, the effect that the size of these flavanols has on the extent of its beneficial properties is also unclear. The objective of this study was to evaluate the dietary effects of cocoa flavanols on the onset of obesity, insulin resistance and impaired glucose tolerance and to determine the impact that the size of these compounds has on the magnitude of this effect. Cocoa extract was fractionated into a monomer-, an oligomer-, and a polymer-rich fraction. Six groups (n=9) of C57BL/6J mice were fed either a control low-fat diet, a control high-fat diet, or a high-fat diet supplemented with 25 mg/kg*BW of cocoa extract or one of the three cocoa fractions. After 12 weeks on these diets, the oligomer-rich fraction proved to be most effective in preventing weight gain, fat mass accumulation, elevated fasting blood glucose and impaired glucose tolerance in diet-induced obese mice. This is the first long-term feeding study to examine the relative activities of cocoa constituents on diet-induced obesity and insulin resistance.iii Acknowledgements

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mentioning

confidence: 99%

Oligomeric Cocoa Procyanidins Possess Enhanced Bioactivity Compared to Monomeric and Polymeric Cocoa Procyanidins for Preventing the Development of Obesity, Insulin Resistance, and Impaired Glucose Tolerance during High-Fat Feeding

Dorenkott

Griffin

Goodrich

et al. 2014

J. Agric. Food Chem.

134

102

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“…This has been the case of isoflavones that has been largely analyzed in food and biological fluids based on their claimed health benefits supposedly due to their antioxidant activity [97,98], a point that is now under controversy. Similar approach has been applied for the advanced analysis of food anthocyanins, isoflavones, and flavanols [99], flavonoids [100], phenolic compounds [101,102], carotenoids [103,104], and other phytochemicals in foods [105]. Many times these studies focus on the biological properties of other compounds, as fatty acids [106] or glucosinolates [107] that can have other sources different than plants.…”

Section: Food Analysis: Current State Of the Art Methodologies And mentioning

confidence: 99%

Food Analysis: Present, Future, and Foodomics

Cifuentes

2012

ISRN Analytical Chemistry

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This paper presents a revision on the instrumental analytical techniques and methods used in food analysis together with their main applications in food science research. The present paper includes a brief historical perspective on food analysis, together with a deep revision on the current state of the art of modern analytical instruments, methodologies, and applications in food analysis with a special emphasis on the works published on this topic in the last three years (2009)(2010)(2011). The article also discusses the present and future challenges in food analysis, the application of "omics" in food analysis (including epigenomics, genomics, transcriptomics, proteomics, and metabolomics), and provides an overview on the new discipline of Foodomics.

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“…Currently, isoflavones have attracted considerable interest from the scientific community. Isoflavones are a subclass of flavonoids, which are described as phytoestrogens because of their estrogenic activity (Valls et al, 2009). They belong to the family of polyphenols, and, in publications and scientific reviews, have been linked to cancer prevention and relief of menopausal, such as prevention of breast cancer (Loibl et al, 2011;Magee et al, 2004;Patisaul & Jefferson, 2010), prevention of prostate cancer (Ganry, 2005), and even prevention of virus infections (Andres et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Development of an analytical method to quantify total isoflavones in phytotherapic capsules using high-performance liquid chromatography

Auwerter¹,

Wanczinski²,

Chiandotti³

2012

Rev. bras. farmacogn.

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Isoflavones can be found in grains and leaves of soybean. Currently, these are sold in pharmacies as phytotherapic capsules. Isoflavones have been recommended by doctors, especially for women, due to their ability to relieve menopause symptoms, among other benefits. However, no method exists for the official control of isoflavone content in capsules sold in the Brazilian market. This study aims to develop an appropriate analytical method to determine the total isoflavone content (daidzin, glycitin, and genistin, and their respective aglycone forms) in phytotherapic capsules purchased in pharmacies in Curitiba, Parana State, Brazil, using the technique of high-performance liquid chromatography with UV detection (UV-HPLC). The HPLC system consisted of a quaternary pump, an autosampler, and Waters reversed-phase C 18 column (5 μm × 300 mm. Analyses were carried out at 40 °C, using a flow rate of 1.0 mL/min (acetonitrile and acetic acid 0.1%), and detection was performed at 254 nm. The method was validated as required by ANVISA and showed to be reliable for the following parameters: linearity (r 2 >0.99), selectivity (correlation between 0.99 and 1.00), precision (relative standard derivation <1.59%), accuracy (from 80% to 111.63% intraday and from 80% to 117.88% interday recovery), and robustness.

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Advanced separation methods of food anthocyanins, isoflavones and flavanols

Cited by 266 publications

References 195 publications

Oligomeric Cocoa Procyanidins Possess Enhanced Bioactivity Compared to Monomeric and Polymeric Cocoa Procyanidins for Preventing the Development of Obesity, Insulin Resistance, and Impaired Glucose Tolerance during High-Fat Feeding

Oligomeric Cocoa Procyanidins Possess Enhanced Bioactivity Compared to Monomeric and Polymeric Cocoa Procyanidins for Preventing the Development of Obesity, Insulin Resistance, and Impaired Glucose Tolerance during High-Fat Feeding

Food Analysis: Present, Future, and Foodomics

Development of an analytical method to quantify total isoflavones in phytotherapic capsules using high-performance liquid chromatography

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