Advanced tissue analysis using a combined histomorphometric and gene expression profiling method

Kriete, Andres; Boyce, Keith

doi:10.1117/12.477875

Cited by 3 publications

(3 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is limited to the degree of visual differentiation that can be achieved by the pathologist and may be affected by diagnostic drift or microscope fatigue. Recently, the use of automated pathology systems to provide the morphologic assessment of the tissue has been investigated to supplement the visual examination by a pathologist (Kriete et al, 2003; Kriete and Boyce, 2003; Young et al, 2003). By using automated tissue scanning microscopes and computer programs designed to identify abnormal tissue morphology, the process of correlating gene expression data and morphologic data may be automated.…”

Section: Comparison Of Morphologic Observations With Data Generated By Other Disciplines In Prechronic/chronic Toxicity Studiesmentioning

confidence: 99%

Comparative Hepatic Toxicity: Prechronic/Chronic Liver Toxicity in Rodents

Hardisty

Brix

2005

Toxicol Pathol

View full text Add to dashboard Cite

The morphologic assessment of the gross and microscopic appearance of the liver can provide a broad base of knowledge concerning the potential toxicity of a drug or chemical. This information may either lead to an understanding of the underlying mechanism of toxicity or guide further study to discern the mode of action of the hepatotoxicity. In standard regulatory bioassays, toxicity studies are conducted during phase 1 and phase 2 of the development process to define the acute, subchronic and chronic toxicity of the test compound. In the liver, there are a limited number of morphologic changes that can be identified using conventional light microscopy. These morphologic alterations are often characterized as "adaptive," consisting of an exaggerated normal physiologic response; "pharmacologic," consisting of an expected alteration in response to the desired action of the test article; or "adverse," consisting of morphologic alterations that are generally undesired, progressive and deleterious to the normal function of the cell(s) involved. Morphologic evidence of adverse effects may involve hepatocytes, the biliary system, hepatic vasculature, Kupffer cells, or stellate cells (Ito cells). In drug discovery and development programs, it is necessary to utilize a multidisciplinary approach, using different endpoints, to investigate the same or similar biological responses in the liver. This results in large amounts of data that must be organized in a retrievable fashion. In order for such a multidisciplinary approach to succeed, each discipline must organize and generate their data in a manner that is easily used by others in the process. The toxicologic pathologist must develop and use standardized nomenclature and diagnostic criteria when examining the liver so that data from various investigators can be compared in a useful manner.

show abstract

Section: Comparison Of Morphologic Observations With Data Generated By Other Disciplines In Prechronic/chronic Toxicity Studiesmentioning

confidence: 99%

Comparative Hepatic Toxicity: Prechronic/Chronic Liver Toxicity in Rodents

Hardisty

Brix

2005

Toxicol Pathol

View full text Add to dashboard Cite

show abstract

“…Using automatic detection, resources could be utilized more efficiently by having the pathologist first only review images binned as abnormal. Several initial studies have demonstrated the advantages of this strategy (Kriete and Boyce, 2003, 2005; Johnson and Braughler, 2003) and software prototypes from the industry have been, or are currently in development (e.g., Definiens AG, Germany; BioImagene Inc., CA; and Systems Pathology Company, LLC, MD). These automated pathology solutions could be very useful for high content analysis, aimed at well-defined specific lesions.…”

Section: New Generation Digital Pathology Approachesmentioning

confidence: 99%

Modern Imaging Technologies in Toxicologic Pathology: An Overview

Ying

Monticello

2006

Toxicol Pathol

View full text Add to dashboard Cite

Modern imaging technology, now utilized in most biomedical research areas (bioimaging), enables the detection and visualization of biological processes at various levels of the molecule, organelle, cell, tissue, organ and/or whole body. In toxicologic pathology, the impact of modern imaging technology is becoming apparent from digital histopathology to novel molecular imaging for in vivo studies. This overview summarizes recent progresses in digital microscopy imaging and newly developed digital slide techniques. Applications of virtual microscopy imaging are discussed and compared to traditional optical microscopy reading. New generation digital pathology approaches, including automatic slide inspection, digital slide databases and image management are briefly introduced. Commonly used in vivo preclinical imaging technologies are also summarized. While most of these new imaging techniques are still undergoing rapid development, it is important that toxicologic pathologists embrace and utilize these technologies as advances occur.

show abstract

“…Ther elationship between gene expression andtissuedatamay be linear or non-linear, in synchronous or asynchronous arrangements.One method employedfor detecting such relationships is based on Manhattan distances [13]. Fort he determination of Manhattandistances, the sumofthe differences of allparameters(allgeneexpression anda ll tissuef eature values) between any time point or treated/disease andc ontrol group is calculated.…”

Section: 5r Evealing Relationships Between Tissueand Gene Expressionsmentioning

confidence: 99%

Automated Tissue Analysis – a Bioinformatics Perspective

Kriete¹,

Boyce²

2005

Methods Inf Med

View full text Add to dashboard Cite

Progress in automated analytics enables the generation of quantitative data about tissue previously limited to visual histopathology. Such reproducible data sets can be statistically correlated and clustered throughout the continuum of bioinformatics. The combined approach supports a system-wide view of biology and has a potential to accelerate developments for a personalized computational diagnosis.

show abstract

Advanced tissue analysis using a combined histomorphometric and gene expression profiling method

Cited by 3 publications

References 3 publications

Comparative Hepatic Toxicity: Prechronic/Chronic Liver Toxicity in Rodents

Comparative Hepatic Toxicity: Prechronic/Chronic Liver Toxicity in Rodents

Modern Imaging Technologies in Toxicologic Pathology: An Overview

Automated Tissue Analysis – a Bioinformatics Perspective

Contact Info

Product

Resources

About