Advancements in Diagnosis and Treatment of Influenza virus

Abstract

Flu is caused by influenza A and B virus leading an acute respiratory illness. This review summarizes advance diagnostic techniques for detecting and confirming influenza virus and treatments involve. RADTS are beneficial tools but with the limited sensitivity. Pandemic confirmation H1N1 influenza A infection done by real-time reverse-transcriptase polymerase chain reaction (rRT-PCR) or viral culture. Several approaches are as of now accessible for determination of flu contaminations like serological tests are generally used to identify flu infection in immunizer reactions incorporate hemagglutination restraint test that depends on capacity of HA-explicit antibodies. Rapid molecular assays that produces results in approximately in 15-30minutes by detecting nucleic acids of influenza in upper respiratory tract. For treating influenza antiviral medications are given to patients based on clinical and epidemiological factors. Neuraminidase inhibitor (NI) is the drug of choice. A good way to prevent the disease is to give individuals annual vaccine and another way is to start an early diagnosis and give medications in short time to patients to prevent further spread of disease and reduce complications otherwise.

Loop-Mediated Isothermal Amplification-Integrated CRISPR Methods for Infectious Disease Diagnosis at Point of Care

“…Moreover, the development of PoC tests for developing countries has remained necessary yet challenging, as such endeavors require long-term and sustained investments. 5 Following the "AS-SURED" criteria set by the World Health Organization (WHO), the optimization of PoC device parameters of (1) affordability, (2) sensitivity, (3) specificity, (4) user-friendliness, (5) rapidness and robustness, (6) equipment-free structure, and (7) delivery 6 has been attempted through microfluidics, 7 antibody-based diagnostic methods, 8 and nucleic acid amplification techniques (NAATs). 9 Loop-mediated isothermal amplification (LAMP) integrated clustered regularly interspaced short palindromic repeat (CRISPR) has emerged as a promising tool to create assays for infectious diseases in order to develop highly sensitive, specific, low-cost, user-friendly, and versatile diagnostic devices.…”