Advancements toward magneto immunoassays

Kriz, K; Gehrke, Janin; Kriz, Dario

doi:10.1016/s0956-5663(98)00047-5

Cited by 68 publications

(31 citation statements)

References 7 publications

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“…The magnetic particles conjugated with biological molecules, which are attractive materials for the assay system, have been proposed for use as a label. They are unaffected by the measurement process and the samples may be stored indefinitely (Kriz et al 1998;Arakaki et al 2004). Furthermore, the magnetic particles can be detected by measuring their magnetism.…”

Section: Magnetic Detection Of Biomolecular Interaction Using Bacmps mentioning

confidence: 99%

Formation of magnetite by bacteria and its application

Arakaki

Nakazawa

Nemoto

et al. 2008

J. R. Soc. Interface.

220

176

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Magnetic particles offer high technological potential since they can be conveniently collected with an external magnetic field. Magnetotactic bacteria synthesize bacterial magnetic particles (BacMPs) with well-controlled size and morphology. BacMPs are individually covered with thin organic membrane, which confers high and even dispersion in aqueous solutions compared with artificial magnetites, making them ideal biotechnological materials. Recent molecular studies including genome sequence, mutagenesis, gene expression and proteome analyses indicated a number of genes and proteins which play important roles for BacMP biomineralization. Some of the genes and proteins identified from these studies have allowed us to express functional proteins efficiently onto BacMPs, through genetic engineering, permitting the preservation of the protein activity, leading to a simple preparation of functional protein-magnetic particle complexes. They were applicable to high-sensitivity immunoassay, drug screening and cell separation. Furthermore, fully automated single nucleotide polymorphism discrimination and DNA recovery systems have been developed to use these functionalized BacMPs. The nano-sized fine magnetic particles offer vast potential in new nano-techniques.

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Section: Magnetic Detection Of Biomolecular Interaction Using Bacmps mentioning

confidence: 99%

Formation of magnetite by bacteria and its application

Arakaki

Nakazawa

Nemoto

et al. 2008

J. R. Soc. Interface.

220

176

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“…Their broad potential for laboratory applications has been demonstrated by their use in detection of typhoid-specific antibodies, cell separation, and antibody sorting (53,(88)(89)(90). More recently, systems using paramagnetic particle labels have been described that make it possible to use iron oxide particles as labels in place of gold (6,49,70,71). The labeled antibodyantigen mixture wicks up the membrane, as described above, and is deposited at the site of the solid-phase antibody, and the magnetic flux is measured in the antigen capture zone.…”

Section: Immunochromatographic Lateral Flow Assaysmentioning

confidence: 99%

Immunological Methods for Detection and Identification of Infectious Disease and Biological Warfare Agents

Peruski

2003

Clin Vaccine Immunol

196

119

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NATURE OF THE PROBLEMBW agents. The release of a biological weapon (BW) agent by a terrorist group or military force would likely be silent and undetectable or nearly so. As shown by anthrax attack during the fall of 2001 in the eastern United States, patients would begin appearing at hospitals and clinics within several days of exposure, most presenting with nonspecific flu-like symptoms. The first days of the outbreak might not even cause undue concern. However, depending on the type of agent and the method of dispersal, the public healthcare system would rapidly be stretched to capacity and beyond.The qualities that make a good BW agent are its relationship between aerosolization, infectivity, or toxicity and the amount of agent required to produce an effect (48). In addition, criteria such as environmental stability, ease of production, disease severity, and communicability determine which agents are the most likely to be utilized. For maximum effect, an optimal agent should be highly lethal and easily produced in large quantities and have limited options for preventive or prophylactic treatment. Given that the respiratory route is the most effective for most BW agents, stability in an aerosol form and the capability to be readily dispersed also in an aerosol (1-to 10-m particle size) are necessary. When potential agents are reviewed for these characteristics, Bacillus anthracis (anthrax) and variola major virus (smallpox) are considered to have the greatest potential for mass casualties and civil disruption. Also high on a prospective list of agents are botulinum neurotoxins, Yersinia pestis, and Francisella tularensis (48,91,92). Lower on the prospective list are Burkholderia pseudomallei and Burkholderia mallei, Rickettsia sp., Coxiella burnetii, Venezuelan equine encephalitis virus, Marburg and Ebola viruses, and influenza viruses (48,63,91,92).Emerging infectious disease agents. In addition to diseases caused by intentional epidemics, there are several emerging infectious diseases (ID) with the potential for significant public health consequences, including dengue fever, West Nile fever, and Rift Valley fever as well as the recent reemergence of malaria in the eastern United States (48,63,91,92). As with BW agents, emerging ID agents may be directly transmissible or vector borne (63). A complex interplay of factors can influence disease emergence, including genetic variation, environmental changes, and population pressures. Further compounding this already complicated situation, are the estimated 600 million international tourists annually, many with the potential to the spread disease globally in a matter of hours (63). Clearly, the challenges facing modern clinical microbiologists and immunologists are daunting enough without the added difficulties posed by the intentional release of BW/ID agents! Because of the threat posed by both BW and emerging or reemerging ID agents, there is a need to rapidly identify such agents in the clinical setting in order to treat the individuals at risk and to improve p...

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“…These commercially available particles are usually 100 nm to 1 m in diameter and comprise a superparamagnetic core surrounded by a polymer shell (e.g., starch or polyvinyl alcohol) suitable for additional functionalization. The superparamagnetic core allows the direct detection and quantification of the particles using a variety of methods (28)(29)(30)(31)(32)(33).…”

Section: Potato Virus X (Pvx) and Tobacco Mosaic Virus (Tmv)mentioning

confidence: 99%

Simple and Portable Magnetic Immunoassay for Rapid Detection and Sensitive Quantification of Plant Viruses

Rettcher

Jungk

Kühn

et al. 2015

Appl Environ Microbiol

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c Plant pathogens cause major economic losses in the agricultural industry because late detection delays the implementation of measures that can prevent their dissemination. Sensitive and robust procedures for the rapid detection of plant pathogens are therefore required to reduce yield losses and the use of expensive, environmentally damaging chemicals. Here we describe a simple and portable system for the rapid detection of viral pathogens in infected plants based on immunofiltration, subsequent magnetic detection, and the quantification of magnetically labeled virus particles. Grapevine fanleaf virus (GFLV) was chosen as a model pathogen. Monoclonal antibodies recognizing the GFLV capsid protein were immobilized onto immunofiltration columns, and the same antibodies were linked to magnetic nanoparticles. GFLV was quantified by immunofiltration with magnetic labeling in a double-antibody sandwich configuration. A magnetic frequency mixing technique, in which a two-frequency magnetic excitation field was used to induce a sum frequency signal in the resonant detection coil, corresponding to the virus concentration within the immunofiltration column, was used for high-sensitivity quantification. We were able to measure GFLV concentrations in the range of 6 ng/ml to 20 g/ml in less than 30 min. The magnetic immunoassay could also be adapted to detect other plant viruses, including Potato virus X and Tobacco mosaic virus, with detection limits of 2 to 60 ng/ml.

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Advancements toward magneto immunoassays

Cited by 68 publications

References 7 publications

Formation of magnetite by bacteria and its application

Formation of magnetite by bacteria and its application

Immunological Methods for Detection and Identification of Infectious Disease and Biological Warfare Agents

Simple and Portable Magnetic Immunoassay for Rapid Detection and Sensitive Quantification of Plant Viruses

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