Advances and applications of oral cancer basic research

Silva, Sabrina Daniela da; Ferlito, Alfio; Takes, Robert P.; Brakenhoff, Ruud H.; Dominguez‐Valentin, Mev; Woolgar, Julia A.; Bradford, Carol R.; Rodrigo, Juan P.; Rinaldo, Alessandra; Hier, Michael P.; Kowalski, Luiz Paulo

doi:10.1016/j.oraloncology.2011.07.004

Cited by 133 publications

(113 citation statements)

References 133 publications

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“…This means that approximately two thirds of patients were diagnosed at an advanced stage, which limits the treatment options and dramatically decreases survival rates. This is consistent with several reports stating late diagnosis as a major problem in managing oral cancer (9,10) .…”

Section: Discussion:-supporting

confidence: 93%

Systemic Markers and Survival of Oral Cancer Patients in Egypt.

Sobeih¹,

Elsharawy²,

Shams³

et al. 2017

IJAR

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Section: Discussion:-supporting

confidence: 93%

Systemic Markers and Survival of Oral Cancer Patients in Egypt.

Sobeih¹,

Elsharawy²,

Shams³

et al. 2017

IJAR

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“…Oral cavity cancer is characterized by high aggression, early recurrence and frequent lymph node metastasis (15).…”

Section: Discussionmentioning

confidence: 99%

Gelsolin regulates proliferation, apoptosis, migration and invasion in human oral carcinoma cells

et al. 2015

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Abstract. Gelsolin (GSN) is one of the most abundant actin-binding proteins, and is involved in several pathological processes, including Alzheimer's disease, cardiac injury and cancer. The aim of the present study was to assess the effect of GSN on the growth and motility of oral squamous cell carcinoma Tca8113 cells. The overexpression vector pcDNA3.1-GSN was transfected into Tca8113 cells and the stable GSN overexpression cell line was identified based on G418 antibiotic selection. The effect of GSN overexpression on the proliferation, apoptosis, migration and invasion of Tca8113 cells was examined using a cell counting kit-8 assay, flow cytometry and Transwell assays. The results revealed that GSN overexpression significantly promoted the cell proliferation and apoptosis of Tca8113 cells. In addition, Transwell assays demonstrated that the migration and invasion abilities of Tca8113 cells were enhanced by GSN overexpression. Therefore, the upregulation of GSN promotes cell growth and motility, indicating that it may perform a vital function in the progression of human oral cancers. IntroductionIn eukaryotic species, the actin cytoskeleton is essential for numerous cellular functions, including maintenance of morphology, motility, division, adhesion, endocytosis, intracellular transport and signal transduction (1-5). The varied and complex activities of the actin cytoskeleton are dynamically regulated by actin-binding proteins (ABPs) (2-4,6,7). Gelsolin (GSN) is one of the most abundant ABPs, and has been found to be a multifunctional regulator of physiological and pathological cellular processes (7,8).Previous studies have indicated that GSN may be a tumor suppressor that exerts a crucial role in the carcinogenic process (7,9). However, biphasic expression of GSN in oral precancerous lesions and oral cancers has been observed, which revealed a downregulation in GSN between oral precancerous lesions and oral cancers, and demonstrated upregulation of GSN in the stages of oral cancer progression (8). The biphasic expression indicated that GSN may perform a more complicated role in oral cancer biology.In order to study the biological roles in oral cancer development in the present study, GSN was overexpressed in oral cancer Tca8113 cells, and the effect of GSN on the proliferation, apoptosis, cell cycle, migration and invasion of these cells was investigated, which may contribute to the present understanding of the biological actions of GSN. Materials and methodsTca8113 cell culture. The human oral squamous cell carcinoma Tca8113 cell line was provided by the Shanghai Ninth People's Hospital, Medical School of Shanghai Jiao Tong University (Shanghai, China). The cells were cultured in RPMI 1640 medium (Gibco-BRL, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS), 100 µg/ml streptomycin and 100 units/ml penicillin, at 37˚C in a 5% CO 2 atmosphere.Stable transfection. To transiently transfect GSN into the Tca8113 cells, 0.5x10 5 cells/well were seeded into a 24-well plate (Corning, Inc., Corning, NY, USA)...

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“…O sítio anatômico mais acometido é a língua e o tipo histológico mais comum é o CEC, o qual representa 90% de todos os casos de câncer de boca (da Silva et al, 2011). Os fatores de risco para o CEC da cavidade oral estão associados principalmente ao consumo de tabaco e álcool.…”

Section: Parte I -Características Gerais Do Cec Bucalunclassified

“…É considerada uma neoplasia de difícil tratamento, o qual geralmente consiste de ressecção cirúrgica acompanhada de radio ou quimioterapia (da Silva et al, 2011). Uma das complicações dos tratamentos convencionais é o aparecimento de radio ou quimiorresistência.…”

Section: Introductionunclassified

Resistência de células de carcinoma epidermóide bucal à terapia fotodinâmica mediada pelo ácido 5-aminolevulínico

Rosin¹

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A minha Orientadora, Prof a . Dr a . Luciana Corrêa, por sempre ouvir e discutir comigo todas as questões e dúvidas que surgiram durante o desenvolvimento desta tese e por ser essa pessoa tão generosa, inteligente e acessível. Admiro-a muito como pessoa e pesquisadora.Ao Prof. Dr. Moacir Novelli, que durante suas visitas ao laboratório sempre alegrava meu dia e por compartilhar comigo um pouco da sua imensa sabedoria. A Marina, a única companheira de profissão da Pós, que sempre esteve disposta a me ajudar e me ouvir, pela amizade, carinho e companheirismo. Pelos nossos cafés da manhã que sempre tornava meu dia mais leve e agradável.A Karla, pelo bom humor constante que sempre alegrava meu dia, por sempre me ouvir, me apoiar e pelos nossos cafezinhos.A Gabriela, por sempre estar ao meu lado me dando força e apoio, pelos nossos almoços diários na copa do departamento que tornavam meu dia mais alegre e por todo carinho e amizade.A Lara, pela amizade e por todo carinho e apoio. Por ser essa pessoa tão agradável e acessível por sempre estar disposta a me ourvir. Tenho a impressão de ter sido uma criança brincando à beira-mar, divertindo-me em descobrir uma pedrinha mais lisa ou uma concha mais bonita que as outras, enquanto o imenso oceano da verdade continua misterioso diante de meus olhos". Oral squamous cell carcinoma (SCC) is a malignant tumor with high morbidity and mortality rates, and it is difficult to treat. Conventional treatment for oral SCCs includes surgery and radiotherapy that may be followed by chemotherapy. Although these treatments are widely used, they are ineffective against some resistant tumors. Isaac Newton RESUMOOncologic photodynamic therapy (PDT) has been used as an adjuvant treatment for oral SCCs, especially in less invasive cases that require tumor reduction before surgical resection. However, like conventional treatments, PDT can induce the occurrence of resistant cell populations such as cutaneous carcinomas and colon and breast adenocarcinomas. The hypothesis that oral SCCs develop resistance to PDThas not yet been tested. Therefore, the aims of this study were to investigate whether oral SCCs (SCC9) develop resistance to several cycles of 5-aminolevulinic acidmediated PDT (5-ALA-PDT) and to determine whether the expression of markers associated with cell survival (NFB, Bcl-2, iNOS, mTOR, and Akt) is altered during this process. An oral SCC (SCC9) cell line was used, which was subjected to the following conditions: 1) Control: cultured without any treatment; 2) ALA: incubated with 5-ALA (1 mM for 4 h); 3) LED: treated with LED light (630 nm, 5.86 J/cm 2 , 22.5 J, 150 mW, 150 s); and 4) PDT: treated with 5-ALA-PDT (with the protocols of the ALA and LED groups combined) generating a lethal dose of 90%. Initially, only one cycle of PDT was administered, and cell viability was determined in all groups 24, 48, 72, and 120 h after irradiation. Subsequently, the DNA fragmentation detection assay (TUNEL) and immunofluorescence analysis of the expression of proteins NFB, Bcl-2, iNOS, pmTOR, ...

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Advances and applications of oral cancer basic research

Cited by 133 publications

References 133 publications

Systemic Markers and Survival of Oral Cancer Patients in Egypt.

Systemic Markers and Survival of Oral Cancer Patients in Egypt.

Gelsolin regulates proliferation, apoptosis, migration and invasion in human oral carcinoma cells

Resistência de células de carcinoma epidermóide bucal à terapia fotodinâmica mediada pelo ácido 5-aminolevulínico

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