Advances and Challenges in the Design and Synthesis of Molecularly Imprinted Microspheres

Lim, K. Fremielle; Zin, Azrinawati Mohd; Romano, Edwin F.; Wanless, Erica J.; Holdsworth, Clovia I.

doi:10.1016/b978-0-12-801301-4.00004-9

Cited by 2 publications

(3 citation statements)

References 139 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The molecular imprinting method has been successfully applied to various molecules, including both small molecules 24 and biological macromolecules. 25,26 However, this method lacks generalizability and is timeconsuming to design a large library for screening. Additionally, substrates produced by molecular imprinting have a lower binding capacity when compared to antibodies and may suffer from incomplete template removal.…”

Section: Introductionmentioning

confidence: 99%

“…However, they are prone to suffer from hydrolysis and degradation, ,, loss of activity after immobilization, low tunability of binding affinity, and have limited availability for a wide range of molecules. , Therefore, extensive research efforts have focused on designing synthetic analogues to natural recognition sites that recognize target analytes with high specificity. ,− One example of such synthetic recognition sites can be constructed with molecular imprinting, − which involves creating cavities in the polymer matrices using the target analyte as a template. The molecular imprinting method has been successfully applied to various molecules, including both small molecules and biological macromolecules. , However, this method lacks generalizability and is time-consuming to design a large library for screening. Additionally, substrates produced by molecular imprinting have a lower binding capacity when compared to antibodies and may suffer from incomplete template removal .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Corona Phase Molecular Recognition of the Interleukin-6 (IL-6) Family of Cytokines Using nIR Fluorescent Single-Walled Carbon Nanotubes

Jin

Lee

Gong

et al. 2023

ACS Appl. Nano Mater.

View full text Add to dashboard Cite

The Interleukin-6 (IL-6) family of cytokines regulates inflammation and plays important roles in numerous biochemical pathways. Typically, cytokine levels are measured using enzyme-linked immunosorbent assay (ELISA) or western blot. However, these techniques usually require substantial processing time, cost, machinery, and specialist training. Understanding the fundamental molecular recognition mechanism of cytokines with synthetic substrates is key to developing new biomedical technologies such as assays, sensors, and therapeutics that overcome the above limitations. Herein, we use the corona phase molecular recognition (CoPhMoRe) approach to engineer new carbon nanotube constructs and study their binding to the inflammatory cytokines: IL-6, interleukin-11 (IL-11), ciliary neurotrophic factor (CNTF), and leukemia inhibitory factor (LIF). Library screening identified two polymer-based CoPhMoRe constructs consisting of single-walled carbon nanotubes complexed with p(AA68-rand-BA16-rand-CD16) polymer (MK2) or p(SS80-rand-BS20) polymer (P14) corona phases. The resulting dissociation constants (K D) were 8.38 ng/mL and 16.7 μg/mL, respectively, compared to that of the natural IL-6 receptor at ∼0.32 ng/mL. In addition, the MK2 constructs showed a nonmonotonic response function upon binding with IL-6. Comparative binding experiments suggest that both constructs appear to recognize the axially aligned α-helical structures present in the Interleukin-6 family. The findings from this study elucidate how nanoparticle interfaces, such as those produced by CoPhMoRe, can be designed to lock onto specific protein features. We find that the α-helical structure of the IL-6 family of cytokines can enable facile molecular recognition, opening the door to new types of label-free, low-cost sensing technologies.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Corona Phase Molecular Recognition of the Interleukin-6 (IL-6) Family of Cytokines Using nIR Fluorescent Single-Walled Carbon Nanotubes

Jin

Lee

Gong

et al. 2023

ACS Appl. Nano Mater.

View full text Add to dashboard Cite

show abstract

“…However, this technique requires large volumes of the polymerization medium, which may hamper molecular interactions [ 47 ]. Finally, the polymerization progress is sensitive to changes in conditions, such as the identity and volume of solvent, formulation, template, and temperature, which would significantly influence the quality of MIPs [ 48 ]. When MIPs are employed as CSPs in practice, they may shrink or swell due to contact with mobile phases.…”

Section: Introductionmentioning

confidence: 99%

Molecularly Imprinted Polymers for the Identification and Separation of Chiral Drugs and Biomolecules

et al. 2016

View full text Add to dashboard Cite

Abstract:Molecularly imprinting polymers (MIPs) have been extensively applied in chromatography for the separation of chiral drugs. In this review, we mainly summarize recent developments of various MIPs used as chiral stationary phases (CSPs) in high performance liquid chromatography (HPLC), capillary electrochromatography (CEC), and supercritical fluid chromatography (SFC). Among them, HPLC has the advantages of straightforward operation and high selectivity. However, the low separation efficiency, due to slow interaction kinetics and heavy peak broadening, is the main challenge for the application of MIPs in HPLC. On the other hand, CEC possesses both the high selectivity of HPLC and the high efficiency of capillary electrophoresis. In CEC, electroosmotic flow is formed across the entire column and reduces the heavy peak broadening observed in HPLC mode. SFC can modify the low interaction kinetics in HPLC when supercritical fluids are utilized as mobile phases. If SFC and MIP-based CSPs can be well combined, better separation performance can be achieved. Particles, monoliths and membrane are typical formats of MIPs. Traditional MIP particles produced by bulk polymerization have been replaced by MIP particles by surface imprinting technology, which are highly consistent in size and shape. Monolithic MIPs are prepared by in situ method in a column, greatly shortening the pre-preparation time. Some novel materials, such as magnetic nanoparticles, are integrated into the MIPs to enhance the controllability and efficiency of the polymerization. This review will be helpful to guide the preparation, development, and application of MIPs in chromatographic and electrophoretic enantioseparation.

show abstract

Advances and Challenges in the Design and Synthesis of Molecularly Imprinted Microspheres

Cited by 2 publications

References 139 publications

Corona Phase Molecular Recognition of the Interleukin-6 (IL-6) Family of Cytokines Using nIR Fluorescent Single-Walled Carbon Nanotubes

Corona Phase Molecular Recognition of the Interleukin-6 (IL-6) Family of Cytokines Using nIR Fluorescent Single-Walled Carbon Nanotubes

Molecularly Imprinted Polymers for the Identification and Separation of Chiral Drugs and Biomolecules

Contact Info

Product

Resources

About