Advances and prospects of platelet-rich plasma therapy in veterinary ophthalmology

Sharun, Khan; Chandran, Deepak; Manjusha, K.M.; Mankuzhy, Pratheesh; Kumar, Rohit; Pawde, A. M.; Dhama, Kuldeep; El-Husseiny, Hussein M.; Amarpal,

doi:10.1007/s11259-022-10064-z

Cited by 15 publications

(16 citation statements)

References 93 publications

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“…Recent breakthroughs in biomedicine and tissue regeneration have significantly broadened the scope of healthcare [ 1 , 2 , 3 ]. Over the years, a wide range of therapeutic modalities and materials have been employed [ 4 , 5 , 6 , 7 , 8 ]. Mesenchymal stem cells (MSCs) are among the highly investigated cell sources for regenerative medicine and tissue engineering applications [ 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Impact of Adipose Tissue Depot Harvesting Site on the Multilineage Induction Capacity of Male Rat Adipose-Derived Mesenchymal Stem Cells: An In Vitro Study

El-Husseiny

Kaneda

Mady

et al. 2023

IJMS

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Recently, substantial attention has been paid toward adipose-derived mesenchymal stem cells (AdMSCs) as a potential therapy in tissue engineering and regenerative medicine applications. Rat AdMSCs (r-AdMSCs) are frequently utilized. However, the influence of the adipose depot site on the multilineage differentiation potential of the r-AdMSCs is still ambiguous. Hence, the main objective of this study was to explore the influence of the adipose tissue harvesting location on the ability of r-AdMSCs to express the stem-cell-related markers and pluripotency genes, as well as their differentiation capacity, for the first time. Herein, we have isolated r-AdMSCs from the inguinal, epididymal, peri-renal, and back subcutaneous fats. Cells were compared in terms of their phenotype, immunophenotype, and expression of pluripotency genes using RT-PCR. Additionally, we investigated their potential for multilineage (adipogenic, osteogenic, and chondrogenic) induction using special stains confirmed by the expression of the related genes using RT-qPCR. All cells could positively express stem cell marker CD 90 and CD 105 with no significant in-between differences. However, they did not express the hematopoietic markers as CD 34 and CD 45. All cells could be induced successfully. However, epididymal and inguinal cells presented the highest capacity for adipogenic and osteogenic differentiation (21.36-fold and 11.63-fold for OPN, 29.69-fold and 26.68-fold for BMP2, and 37.67-fold and 22.35-fold for BSP, respectively, in epididymal and inguinal cells (p < 0.0001)). On the contrary, the subcutaneous cells exhibited a superior potential for chondrogenesis over the other sites (8.9-fold for CHM1 and 5.93-fold for ACAN, (p < 0.0001)). In conclusion, the adipose tissue harvesting site could influence the differentiation capacity of the isolated AdMSCs. To enhance the results of their employment in various regenerative cell-based therapies, it is thus vital to take the collection site selection into consideration.

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Section: Introductionmentioning

confidence: 99%

Impact of Adipose Tissue Depot Harvesting Site on the Multilineage Induction Capacity of Male Rat Adipose-Derived Mesenchymal Stem Cells: An In Vitro Study

El-Husseiny

Kaneda

Mady

et al. 2023

IJMS

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“…The basic goal of TE is to repair damaged tissues and replace them with new biological ones ( Gaspar et al, 2020 ; Lavrador et al, 2021 ; El-Husseiny et al, 2022c ; El-Husseiny et al, 2022d ; El-Husseiny et al, 2022f ; Sharun et al, 2023 ). Cell biology and biochemistry are required for this interdisciplinary process ( El-Husseiny et al, 2022d ; Doghish et al, 2023 ; El-Mahdy et al, 2023 ; Mady et al, 2023 ; Sharun et al, 2023 ). For clinical applications, clinical medical and material sciences investigations are also included ( Gaspar et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Stimuli-responsive hydrogels: smart state of-the-art platforms for cardiac tissue engineering

El-Husseiny

Mady

El-Dakroury

et al. 2023

Front. Bioeng. Biotechnol.

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Biomedicine and tissue regeneration have made significant advancements recently, positively affecting the whole healthcare spectrum. This opened the way for them to develop their applications for revitalizing damaged tissues. Thus, their functionality will be restored. Cardiac tissue engineering (CTE) using curative procedures that combine biomolecules, biomimetic scaffolds, and cells plays a critical part in this path. Stimuli-responsive hydrogels (SRHs) are excellent three-dimensional (3D) biomaterials for tissue engineering (TE) and various biomedical applications. They can mimic the intrinsic tissues’ physicochemical, mechanical, and biological characteristics in a variety of ways. They also provide for 3D setup, adequate aqueous conditions, and the mechanical consistency required for cell development. Furthermore, they function as competent delivery platforms for various biomolecules. Many natural and synthetic polymers were used to fabricate these intelligent platforms with innovative enhanced features and specialized capabilities that are appropriate for CTE applications. In the present review, different strategies employed for CTE were outlined. The light was shed on the limitations of the use of conventional hydrogels in CTE. Moreover, diverse types of SRHs, their characteristics, assembly and exploitation for CTE were discussed. To summarize, recent development in the construction of SRHs increases their potential to operate as intelligent, sophisticated systems in the reconstruction of degenerated cardiac tissues.

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“…Employment of the ECM generated from decellularized tissues is a leading strategy in regenerative medicine [ 19 ]. It affects cellular differentiation, mitogenesis, chemotaxis, and tissue-remodeling responses [ 19 , 20 , 21 , 22 , 23 , 24 , 25 ]. Decellularization also lessens antigenicity, especially in xenografts, which is a very important feature [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

Comparison of Bovine- and Porcine-Derived Decellularized Biomaterials: Promising Platforms for Tissue Engineering Applications

et al. 2023

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Animal-derived xenogeneic biomaterials utilized in different surgeries are promising for various applications in tissue engineering. However, tissue decellularization is necessary to attain a bioactive extracellular matrix (ECM) that can be safely transplanted. The main objective of the present study is to assess the structural integrity, biocompatibility, and potential use of various acellular biomaterials for tissue engineering applications. Hence, a bovine pericardium (BP), porcine pericardium (PP), and porcine tunica vaginalis (PTV) were decellularized using a Trypsin, Triton X (TX), and sodium dodecyl sulfate (SDS) (Trypsin + TX + SDS) protocol. The results reveal effective elimination of the cellular antigens with preservation of the ECM integrity confirmed via staining and electron microscopy. The elasticity of the decellularized PP (DPP) was markedly (p < 0.0001) increased. The tensile strength of DBP, and DPP was not affected after decellularization. All decellularized tissues were biocompatible with persistent growth of the adipose stem cells over 30 days. The staining confirmed cell adherence either to the peripheries of the materials or within their matrices. Moreover, the in vivo investigation confirmed the biocompatibility and degradability of the decellularized scaffolds. Conclusively, Trypsin + TX + SDS is a successful new protocol for tissue decellularization. Moreover, decellularized pericardia and tunica vaginalis are promising scaffolds for the engineering of different tissues with higher potential for the use of DPP in cardiovascular applications and DBP and DPTV in the reconstruction of higher-stress-bearing abdominal walls.

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Advances and prospects of platelet-rich plasma therapy in veterinary ophthalmology

Cited by 15 publications

References 93 publications

Impact of Adipose Tissue Depot Harvesting Site on the Multilineage Induction Capacity of Male Rat Adipose-Derived Mesenchymal Stem Cells: An In Vitro Study

Impact of Adipose Tissue Depot Harvesting Site on the Multilineage Induction Capacity of Male Rat Adipose-Derived Mesenchymal Stem Cells: An In Vitro Study

Stimuli-responsive hydrogels: smart state of-the-art platforms for cardiac tissue engineering

Comparison of Bovine- and Porcine-Derived Decellularized Biomaterials: Promising Platforms for Tissue Engineering Applications

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