Advances in Cytokinesis Research. Cytokinesis in Budding Yeast: The Relationship between Actomyosin Ring Function and Septum Formation.

Bi, Erfei

doi:10.1247/csf.26.529

Cited by 66 publications

(100 citation statements)

References 82 publications

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“…Cytokinesis in S. cerevisiae is carried out by the coordinated actions of the actomyosin-based contractile ring and septum formation (Bi 2001;Finger 2005). Proteins involved in cytokinesis, such as Myo1, Iqg1, and Hof1, target to the bud neck in a septin-dependent manner (Bi et al 1998;Lippincott and Li 1998;Vallen et al 2000;Bi 2001;Gladfelter et al 2001b), but it is not clear how individual septins contribute to this targeting process.…”

Section: Shs1 Acts Differently In Cytokinesis From Other Septinsmentioning

confidence: 99%

“…Proteins involved in cytokinesis, such as Myo1, Iqg1, and Hof1, target to the bud neck in a septin-dependent manner (Bi et al 1998;Lippincott and Li 1998;Vallen et al 2000;Bi 2001;Gladfelter et al 2001b), but it is not clear how individual septins contribute to this targeting process. Here, we showed that Iqg1 and Myo1 were delocalized or mislocalized from the bud neck in shs1D cells in spite of the presence of other septins at the bud neck.…”

Section: Shs1 Acts Differently In Cytokinesis From Other Septinsmentioning

confidence: 99%

“…Many proteins of budding yeast are known to localize to the bud neck in a septin-dependent manner (Bi 2001;Gladfelter et al 2001b). Those are proteins functioning in septin recruitment and ring assembly (see above), cytokinesis (see below), bud-site selection (Chant et al 1995;Sanders and Herskowitz 1996;Kang et al 2004), cell cycle control (Frenz et al 2000;Yoshida and Toh-e 2001), and mitotic signaling network (Carroll et al 1998;Longtine et al 1998Longtine et al , 2000Barral et al 1999;Lee et al 2002;.…”

mentioning

confidence: 99%

“…Myo1, Hof1, and Cyk3 target independently to the septin ring and seem to carry out their roles in cytokinesis independently since deletion of any two causes cell lethality (Vallen et al 2000;Korinek et al 2000). However, in wild-type cells, the components of the actomyosin ring and septum formation must coordinate with each other in time and space so that cytokinesis can be carried out most efficiently (Bi 2001). …”

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confidence: 99%

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Shs1 Plays Separable Roles in Septin Organization and Cytokinesis in Saccharomyces cerevisiae

Iwase

Luo

et al. 2007

Genetics

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In Saccharomyces cerevisiae, five septins (Cdc3, Cdc10, Cdc11, Cdc12, and Shs1/Sep7) form the septin ring at the bud neck during vegetative growth. We show here that disruption of SHS1 caused cold-sensitive growth in the W303 background, with cells arrested in chains, indicative of a cytokinesis defect. Surprisingly, the other four septins appeared to form an apparently normal septin ring in shs1D cells grown under the restrictive condition. We found that Myo1 and Iqg1, two components of the actomyosin contractile ring, and Cyk3, a component of the septum formation, were either delocalized or mislocalized in shs1D cells, suggesting that Shs1 plays supportive roles in cytokinesis. We also found that deletion of SHS1 enhanced or suppressed the septin defect in cdc10D and cdc11D cells, respectively, suggesting that Shs1 is involved in septin organization, exerting different effects on septin-ring assembly, depending on the composition of the septin subunits. Furthermore, we constructed an shs1-100c allele that lacks the coding sequence for the C-terminal 32 amino acids. This allele still displayed the genetic interactions with the septin mutants, but did not show cytokinesis defects as described above, suggesting that the roles of Shs1 in septin organization and cytokinesis are separable.

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Section: Shs1 Acts Differently In Cytokinesis From Other Septinsmentioning

confidence: 99%

Section: Shs1 Acts Differently In Cytokinesis From Other Septinsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Shs1 Plays Separable Roles in Septin Organization and Cytokinesis in Saccharomyces cerevisiae

Iwase

Luo

et al. 2007

Genetics

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“…In wild-type cells, newly synthesised cell wall material is deposited centripetally at the neck that separates mother cell and bud (bud neck) to form a septum. After cytokinesis, the septum is split down the middle allowing the cells to separate (6). Cytokinesis is delayed in vrp1D cells even at 24 8C.…”

Section: Introductionmentioning

confidence: 99%

Verprolin: A cool set of actin‐binding sites and some very HOT prolines

Munn

Thanabalu

2009

IUBMB Life

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SummarySpatiotemporal organisation of eukaryotic cells is established and maintained by the cytoskeleton, a highly dynamic and complex network of structural and signalling proteins. Many components of the cytoskeleton are functionally and structurally conserved between humans and yeast. Among these are verprolin (Vrp1p) in yeast and its human ortholog Wiskott-Aldrich syndrome protein (WASP)-interacting protein (WIP). Much of our understanding of the function of these proteins has come from genetic analysis in yeast. Verprolin-deficient yeast cells exhibit defects in cytokinesis, endocytosis, and actin cytoskeleton polarisation. Verprolin binds actin, the yeast ortholog of human WASP (Las17p or Bee1p), and the yeast ortholog of human PSTPIP1 (Hof1p or Cyk2p). We propose that verprolin acts as a chaperone that by transient bimolecular interactions maintains the proper function of its partners. Verprolin-related proteins and partners are implicated in cancer, immunodeficiency, and neurodegeneration. Therefore, elucidating how verprolin functions will have major impacts in cell biology and medicine.2009 IUBMB IUBMB Life, 61(7): [707][708][709][710][711][712] 2009

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Mutation of Hof1 PEST motif phosphorylation sites leads to retention of Hof1 at the bud neck and a decrease in the rate of myosin contraction

Stockstill

Park

Wille

et al. 2013

Cell Biology International

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Regulation of actomyosin ring contraction is important for the coordination of cytokinesis with mitosis. Hof1, a member of the Pombe Cdc15 homology (PCH) family of proteins, is required for efficient cytokinesis in budding yeast. Phosphorylation of Hof1 depends on the mitotic exit network (MEN), and its degradation at the end of mitosis depends on its PEST motif and interaction with the E3 ligase Grr1. To test the hypothesis that targeted destruction of Hof1 temporally couples mitotic exit with contraction of the actomyosin ring, we mutated the Hof1 PEST motif to prevent phosphorylation and subsequent degradation. These mutations increased the amount of Hof1 at the bud neck during cytokinesis, resulted in smaller bud neck diameter, and slowed the rate of myosin contraction. However, Hof1 PEST motif phosphorylation site mutants did not have cytokinesis defects, indicating that regulation of Hof1 levels does not control the onset of actomyosin ring contraction as predicted.

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Advances in Cytokinesis Research. Cytokinesis in Budding Yeast: The Relationship between Actomyosin Ring Function and Septum Formation.

Cited by 66 publications

References 82 publications

Shs1 Plays Separable Roles in Septin Organization and Cytokinesis in Saccharomyces cerevisiae

Shs1 Plays Separable Roles in Septin Organization and Cytokinesis in Saccharomyces cerevisiae

Verprolin: A cool set of actin‐binding sites and some very HOT prolines

Mutation of Hof1 PEST motif phosphorylation sites leads to retention of Hof1 at the bud neck and a decrease in the rate of myosin contraction

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