Advances in Cytokinesis Research. The Molecular Mechanism of Targeted Vesicle Transport in Cytokinesis.

Edamatsu, Masaki

doi:10.1247/csf.26.567

Cited by 6 publications

(5 citation statements)

References 37 publications

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“…Because fusion of a wide tubular network with the plasma membrane triggers callose formation within the tubules and their conversion into convoluted cell plate sheets, post-meiotic cell plate maturation occurs in a centripetal direction. However, these inward-growing cell plate domains do not correspond to cleavage furrows, because their growth is mediated by fusion with preformed cell plate intermediates, the wide tubular networks, and not by the addition of individual secretory vesicles as described for cleavage furrow growth (Edamatsu 2001). Recently cellularized microspore tetrad.…”

Section: Discussionmentioning

confidence: 99%

Electron tomographic analysis of post-meiotic cytokinesis during pollen development in Arabidopsis thaliana

Otegui

Staehelin

2004

Planta

107

102

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The mechanism of cell wall formation after male meiosis was studied in microsporocytes of Arabidopsis thaliana (L.) Heynh. by means of thin-section and immuno-electron microscopy and dual-axis electron tomography of high-pressure-frozen/freeze-substituted cells. The cellularization of four-nucleate microsporocytes involves a novel type of cell plate, called a post-meiotic-type cell plate. As in the syncytial endosperm, the microsporocyte cell plates assemble in association with mini-phragmoplasts. However, in contrast to the endosperm cell plates, post-meiotic type cell plates arise simultaneously across the entire division plane. Vesicles are transported along mini-phragmoplast microtubules by putative kinesin proteins and, prior to fusion, they become connected together by 24-nm-long linkers that resemble exocyst complexes. These vesicles fuse with each other to form wide tubules and wide tubular networks. In contrast to endosperm cell plates, the wide tubular networks in microsporocytes completely lack callose and do not appear to be constricted by dynamin rings. The most peripheral wide tubular networks begin to fuse with the plasma membrane before the more central cell plate assembly sites become integrated into a coherent cell plate. Fusion with the parental plasma membrane triggers callose synthesis and the wide tubular domains are converted into convoluted sheets. As the peripheral convoluted sheets accumulate callose and arabinogalactan proteins, they are converted into stub-like projections, which grow centripetally, i.e. toward the interior of the syncytium, fusing with the wide tubular networks already assembled in the division plane. We also demonstrate that the ribosome-excluding cell plate assembly matrix is delivered to the mini-phragmoplast with the first vesicles, and encompasses all the linked vesicles and intermediate stages in cell plate formation.

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Section: Discussionmentioning

confidence: 99%

Electron tomographic analysis of post-meiotic cytokinesis during pollen development in Arabidopsis thaliana

Otegui

Staehelin

2004

Planta

107

102

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“…, 2005). Phragmoplast expansion and function are regulated at three levels: MT dynamics, vesicle delivery, and the direction of expansion (Edamatsu, 2001; Hasezawa & Kumagai, 2002; Sasabe & Machida, 2006; Van Damme & Geelen, 2008).…”

Section: Introductionmentioning

confidence: 99%

Mitogen‐activated protein kinase 4 is involved in the regulation of mitotic and cytokinetic microtubule transitions in Arabidopsis thaliana

et al. 2010

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Summary• A mitogen-activated protein kinase kinase kinase (MAPKKK) double mutant, Arabidopsis homologue of nucleus and phragmoplast associated kinase (anp) anp2anp3, and the mitogen-activated protein kinase (MAPK) 4 mutant mpk4 of Arabidopsis thaliana show prominent cytokinetic defects. This prompted the analysis of mitotic and cytokinetic progression as a function of MAPK signalling. Mutants were compared with wild types untreated or treated with the specific MAPKK inhibitor PD98059.• This study included phenotype analysis, expression analysis of the MPK4 promoter, immunofluorescent localization of MPK4, tubulin and MAP65-1, and time-lapse microscopic visualization of the mitotic microtubule (MT) transitions in control, mutant and inhibitor-treated cells.• Mutant and inhibitor-treated cells showed defects in mitosis and cytokinesis, including aberrant spindle and phragmoplast formation and drastically delayed or abortive mitosis and cytokinesis. As a result, bi-and multinucleate cells were formed, ultimately disturbing the vegetative tissue patterning. MPK4 was localized to all stages of the expanding phragmoplast, in a pattern similar to that of its putative substrate MAP65-1.• In this study, MPK4 is shown to be involved in the regulation of mitosis ⁄ cytokinesis through modulation of the cell division plane and cytokinetic progression.

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“…In principle, such membrane could arrive either by cortical flow from the poles of the cell (Wang et al, 1994) or by membrane addition in the vicinity of the ingressing furrow. Indeed, furrow-associated vesicle fusion events at the plasma membrane promote cleavage of frog (Xenopus laevis) and worm (Caenorhabditis elegans) embryos and promote cellularization in fruit fly (Drosophila melanogaster) embryos (Burgess et al, 1997;Byers and Armstrong, 1986;Jantsch-Plunger and Glotzer, 1999;Skop et al, 2001) (reviewed by Edamatsu, 2001;Hales et al, 1999;Straight and Field, 2000). Many types of membrane transaction are important during cleavage, as mutations in proteins affecting endocytosis (e.g., dynamin, clathrin), secretion (syntaxin 1, syntaxin 5, COG5), lysosomal trafficking (lvsA) or recycling endosomes (Rab11, Nuf) cause cytokinesis or cellularization defects in various organisms (Farkas et al, 2003;Gerald et al, 2001; Kang et al, 2003;Kwak et al, 1999;Lauber et al, 1997;Riggs et al, 2003;Thompson et al, 2002;Xu et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Continuous phosphatidylinositol metabolism is required for cleavage of crane fly spermatocytes

Saul

Fabian

Forer

et al. 2004

Journal of Cell Science

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Successful cleavage of animal cells requires co-ordinated regulation of the actomyosin contractile ring and cleavage furrow ingression. Data from a variety of systems implicate phosphoinositol lipids and calcium release as potential regulators of this fundamental process. Here we examine the requirement for various steps of the phosphatidylinositol (PtdIns) cycle in dividing crane fly (Nephrotoma suturalis) spermatocytes. PtdIns cycle inhibitors were added to living cells after cleavage furrows formed and began to ingress. Inhibitors known to block PtdIns recycling (lithium), PtdIns phosphorylation (wortmannin, LY294002) or phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2] hydrolysis [U73122 (U7)] all stopped or slowed furrowing. The effect of these drugs on cytokinesis was quite rapid (within 0-4 minutes), so continuous metabolism of PtdIns appears to be required for continued cleavage furrow ingression. U7 caused cleavage furrow regression concomitant with depletion of F-actin from the contractile ring, whereas the other inhibitors caused neither regression nor depletion of F-actin. That U7 depletes furrow-associated actin seems counterintuitive, as inhibition of phospholipase C would be expected to increase cellular levels of PtdIns(4,5)P2 and hence increase actin polymerization. Our confocal images suggest, however, that F-actin might accumulate at the poles of U7-treated cells, consistent with the idea that PtdIns(4,5)P2 hydrolysis may be required for actin filaments formed at the poles to participate in contractile ring assembly at the furrow.

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Advances in Cytokinesis Research. The Molecular Mechanism of Targeted Vesicle Transport in Cytokinesis.

Cited by 6 publications

References 37 publications

Electron tomographic analysis of post-meiotic cytokinesis during pollen development in Arabidopsis thaliana

Electron tomographic analysis of post-meiotic cytokinesis during pollen development in Arabidopsis thaliana

Mitogen‐activated protein kinase 4 is involved in the regulation of mitotic and cytokinetic microtubule transitions in Arabidopsis thaliana

Continuous phosphatidylinositol metabolism is required for cleavage of crane fly spermatocytes

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