Advances in Electromagnetic Piezoelectric Acoustic Sensor Technology for Biosensor-Based Detection

Mészáros, Gábor; Akbarzadeh, Sanaz; Franier, Brian De La; Keresztes, Zsófia; Thompson, Michael

doi:10.3390/chemosensors9030058

Cited by 9 publications

(5 citation statements)

References 24 publications

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“…The first biosensor study of the marker HSP10 was conducted using the electromagnetic piezoelectric acoustic wave (EMPAS) device [163]. This sensor involves the instigation of acoustic waves in a quartz substrate via the secondary electric field developed by a radio frequency-excited flat spiral coil [164]. The main focus of this investigation was to examine the interactive chemistry for the hexahistidine-tagged protein attached to the device (quartz) via NTAchemistry with a selective aptamer produced by conventional SELEX protocols, 5′-AACTGGTGCGGGGTGGTGGGGGATGGATGTTGCTTG AGGGGTC -3′.…”

Section: Sensors Ovarian Cancermentioning

confidence: 99%

“…163 This sensor involves the instigation of acoustic waves in a quartz substrate via the secondary electric field developed by a radio frequency-excited flat spiral coil. 164 The main focus of this investigation was to examine the interactive chemistry for the hexa-histidine-tagged protein attached to the device (quartz) via NTAchemistry with a selective aptamer produced by conventional SELEX protocols, 5′-AACTGGTGCGGGGTGGTGGGGGATGGATGTTGCTTG AGGG GTC-3′. Experiments conducted at 940 MHz frequency did Sensors & Diagnostics Critical review reveal a response for protein-nucleic acid binding, which was attributed to a rigidification of the complex on the quartz surface.…”

Section: Sensors and Diagnosticsmentioning

confidence: 99%

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Sensor detection in gynaecological medicine

et al. 2022

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Section: Sensors Ovarian Cancermentioning

confidence: 99%

Section: Sensors and Diagnosticsmentioning

confidence: 99%

Sensor detection in gynaecological medicine

et al. 2022

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“…At present, many transducing methods are being developed or utilized toward biosensing, such as plasmonic, 7 electrochemical, 8 thermometric, 9 piezoelectric, 10 and magnetic methods. 11 Among them, optical fiber-based biochemical sensors have attracted huge attention from the science community because of the outstanding features of optical fibers like compact size, superior biocompatibility, remote operation potential, and multiplexing detection capability.…”

Section: Introductionmentioning

confidence: 99%

Label-Free Biochemical Sensing Using Processed Optical Fiber Interferometry: A Review

Jha,

Gorai,

Shrivastav

et al. 2024

ACS Omega

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Over the last 20 years, optical fiber-based devices have been exploited extensively in the field of biochemical sensing, with applications in many specific areas such as the food processing industry, environmental monitoring, health diagnosis, bioengineering, disease diagnosis, and the drug industry due to their compact, label-free, and highly sensitive detection. The selective and accurate detection of biochemicals is an essential part of biosensing devices, which is to be done through effective functionalization of highly specific recognition agents, such as enzymes, DNA, receptors, etc., over the transducing surface. Among many optical fiber-based sensing technologies, optical fiber interferometry-based biosensors are one of the broadly used methods with the advantages of biocompatibility, compact size, high sensitivity, high-resolution sensing, lower detection limits, operating wavelength tunability, etc. This Review provides a comprehensive review of the fundamentals as well as the current advances in developing optical fiber interferometry-based biochemical sensors. In the beginning, a generic biosensor and its several components are introduced, followed by the fundamentals and state-of-art technology behind developing a variety of interferometry-based fiber optic sensors. These include the Mach−Zehnder interferometer, the Michelson interferometer, the Fabry−Perot interferometer, the Sagnac interferometer, and biolayer interferometry (BLI). Further, several technical reports are comprehensively reviewed and compared in a tabulated form for better comparison along with their advantages and disadvantages. Further, the limitations and possible solutions for these sensors are discussed to transform these in-lab devices into commercial industry applications. At the end, in conclusion, comments on the prospects of field development toward the commercialization of sensor technology are also provided. The Review targets a broad range of audiences including beginners and also motivates the experts helping to solve the real issues for developing an industry-oriented sensing device.

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“…The EMPAS system was first developed in the early 2000s in order to perform acoustic wave measurements at ultra-high frequencies in the several hundred MHz [8]. This system has been extensively studied with regards to anti-fouling coatings on silica using trichlorosilanes-based layers as a method of surface adhesion [9,10]. More recent work has involved updating the EMPAS system to operate on more modern computer hardware making it easier to operate (Figure 1) [11].…”

Section: Introductionmentioning

confidence: 99%

Interaction of Pseudomonas aeruginosa with surface-modified silica studied by ultra-high frequency acoustic wave biosensor

De La Franier,

Thompson

2024

Explor BioMat-X

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Aim: This study aimed to examine the amount of surface non-specific adsorption, or fouling, observed by Pseudomonas aeruginosa (P. aeruginosa) on a quartz crystal based acoustic wave biosensor under different flow conditions with and without an anti-fouling layer. Methods: An electromagnetic piezoelectric acoustic sensor (EMPAS) based on electrode free quartz crystals was used to perform the analysis. Phosphate buffered saline (PBS) was flowed over the crystal surface at various flow rates from 50 μL/min to 200 μL/min, with measurements being taken at the 43rd harmonic (~864 MHz). The crystal was either unmodified, or modified with a monoethylene glycol [2-(3-silylpropyloxy)-hydroxy-ethyl (MEG-OH)] anti-fouling layer. Overnight culture of P. aeruginosa PAO1 (PAO1) in lysogeny broth (LB) was injected into the system, and flow maintained for 30 min. Results: The frequency change of the EMPAS crystal after injection of bacteria into the system was found to change based on the flow rate of buffer, suggesting the flow rate has a strong effect on the level of non-specific adsorption. The MEG-OH layer drastically reduced the level of fouling observed under all flow conditions, as well as reduced the amount of variation between experiments. Flow rates of 150 μL/min or higher were found to best reduce the level of fouling observed as well as experimental variation. Conclusions: The MEG-OH anti-fouling layer is important for accurate and reproducible biosensing measurements due to the reduced fouling and variation during experiments. Additionally, a flow rate of 150 μL/min may prove better for measurement compared to the current standard of 50 μL/min for this type of instrument.

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Advances in Electromagnetic Piezoelectric Acoustic Sensor Technology for Biosensor-Based Detection

Cited by 9 publications

References 24 publications

Sensor detection in gynaecological medicine

Sensor detection in gynaecological medicine

Label-Free Biochemical Sensing Using Processed Optical Fiber Interferometry: A Review

Interaction of Pseudomonas aeruginosa with surface-modified silica studied by ultra-high frequency acoustic wave biosensor

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