Advances in methodology for immunodiagnosis of parasitic diseases

Fife, Earl H.

doi:10.1016/0014-4894(71)90079-8

Cited by 28 publications

(4 citation statements)

References 126 publications

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“…Schistosome ES proteins have been shown to play an important role in modulating host immune responses (16). The present study involved preparation of ES antigen against the whole worm homogenates because ES antigens evoke a considerably higher degree of protective immunity in lab animals than the somatic antigen derived from whole worm (24). Lack of cross reactivity and the diagnostic specificity of ES antigen was confirmed and strong recommendation for the incorporation of ES antigen in various immunodiagnostic assays for detection of S. spindale was advised (17).…”

Section: Discussionmentioning

confidence: 99%

Development and Evaluation of a Colloidal Gold Immunofiltration Assay for Penside Diagnosis of Visceral Schistosomosis in Cattle

Mamane

Jeyathilakan

Latha

et al. 2022

Preprint

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BackgroundVisceral schistosomosis caused by Schistosoma spindale is an economically important chronic wasting snail-borne trematode infection which often goes undiagnosed in field condition due to lack of efficient diagnostic assays. ELISA and Colloidal gold immunofiltration assay were used to evaluate the state of active visceral schistosomosis in cattle through detection of antibodies against S. spindale ES antigen.Methodology/Principle FindingsThe present study included development and evaluation of enzyme linked immunosorbent assay (ELISA) and colloidal gold immunofiltration assay (CGIFA) with conventionally prepared S. spindale excretory-secretory (ES) antigen using postmortem mesenteric examination as a reference standard. Statistical analysis was performed using Cochran Q test, Chi-square test and kappa statistics which showed no significant difference between the developed tests and strong agreement with the postmortem examination which was considered as gold standard test. CGIFA showed sensitivity, specificity and accuracy of 92.98 %, 96.74 % and 95.55 % respectively whereas that of ELISA was 94.73 %, 95.12 % and 95% respectively.Conclusion/SignificanceCGIFA was found to be simpler, rapid, effective, less expensive and required least expertise for interpretation of results. It can be used as portable diagnostic device for rapid diagnosis of S. spindale infection at field condition making it an ideal pen side diagnostic kit.

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Section: Discussionmentioning

confidence: 99%

Development and Evaluation of a Colloidal Gold Immunofiltration Assay for Penside Diagnosis of Visceral Schistosomosis in Cattle

Mamane

Jeyathilakan

Latha

et al. 2022

Preprint

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“…In the absence of parasitological evidence of infection, diagnosis of larval toxocariasis has relied mainly on immunological methods. Highly sensitive and specific serodiagnostic assays have been developed using Toxocara excretorysecretory (ES) antigens (Fife, 1971;de Savigny, 1975;Glickman et al, 1985). The large-scale product of this antigen is recovery of Toxocara spp.…”

Section: Introductionmentioning

confidence: 99%

In vitro cultivation of Toxocara cati adult worms for production of eggs and evaluation of oviposition

Zibaei¹,

Sadjjadi

Sarkari

et al. 2009

Helminthologia

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28 SummaryToxocariasis is the clinical syndrome caused by infection of zoonotic roundworms of dogs (Toxocara canis) or cats (Toxocara cati). Current research on the immunology and pathology aspects of toxocariasis requires Toxocara second stage larvae and a ready source of excretorysecretory (ES) antigens. We cultured eleven pairs of both sexes of Toxocara cati adult worms maintained in RPMI 1640 medium in order to evaluate the amounts and duration of egg laying. At the first day and last day (day 19), the mean egg counts were 9300 and 250 eggs/ml, respectively. These results showed that this culture method is very appropriate for collection of pure oviposited eggs and/or production of adult ES antigens of Toxocara cati that could be used for immunological diagnosis of toxocariasis.

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“…immunological reactions of hosts to helminth infection, dealt mainly with the development and improvement of procedures for the immunodiagnosis of helminthosis (e.g., the intradermal test for human hydatidosis developed by Casoni in 1911), and this particular aspect of host immunity has be.en reviewed by Fife (1971).…”

Section: Introductionmentioning

confidence: 99%

Immunity and epidemiology of helminthiasis in grazing animals

1973

New Zealand Veterinary Journal

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Advances in methodology for immunodiagnosis of parasitic diseases

Cited by 28 publications

References 126 publications

Development and Evaluation of a Colloidal Gold Immunofiltration Assay for Penside Diagnosis of Visceral Schistosomosis in Cattle

Development and Evaluation of a Colloidal Gold Immunofiltration Assay for Penside Diagnosis of Visceral Schistosomosis in Cattle

In vitro cultivation of Toxocara cati adult worms for production of eggs and evaluation of oviposition

Immunity and epidemiology of helminthiasis in grazing animals

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