Advances in Nutrigenomics research: Novel and future analytical approaches to investigate the biological activity of natural compounds and food functions

García‐Cañas, Virginia; Simó, Carolina; León, Carlos; Cifuentes, Alejandro

doi:10.1016/j.jpba.2009.04.019

Cited by 104 publications

(61 citation statements)

References 188 publications

(182 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Alterations of the NADH/NAD + ratio could also affect chromatin structure through the action of sirtuins, a class of HDACs that are known to be NAD + dependent (28). Advocates of individualized medicine have predicted therapeutic benefit of targeted dietary interventions, although currently there are few robust examples (29)(30)(31). This work serves as a proof-of-principle that dietary manipulation may be a feasible strategy for KS and suggests a possible mechanism of action of the previously observed therapeutic benefits of the KD for intractable seizure disorder (22,23).…”

Section: Discussionmentioning

confidence: 99%

A ketogenic diet rescues hippocampal memory defects in a mouse model of Kabuki syndrome

Benjamin

Pilarowski

Carosso

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

120

View full text Add to dashboard Cite

Kabuki syndrome is a Mendelian intellectual disability syndrome caused by mutations in either of two genes (KMT2D and KDM6A) involved in chromatin accessibility. We previously showed that an agent that promotes chromatin opening, the histone deacetylase inhibitor (HDACi) AR-42, ameliorates the deficiency of adult neurogenesis in the granule cell layer of the dentate gyrus and rescues hippocampal memory defects in a mouse model of Kabuki syndrome (Kmt2d+/βGeo). Unlike a drug, a dietary intervention could be quickly transitioned to the clinic. Therefore, we have explored whether treatment with a ketogenic diet could lead to a similar rescue through increased amounts of beta-hydroxybutyrate, an endogenous HDACi. Here, we report that a ketogenic diet in Kmt2d+/βGeo mice modulates H3ac and H3K4me3 in the granule cell layer, with concomitant rescue of both the neurogenesis defect and hippocampal memory abnormalities seen in Kmt2d+/βGeo mice; similar effects on neurogenesis were observed on exogenous administration of beta-hydroxybutyrate. These data suggest that dietary modulation of epigenetic modifications through elevation of beta-hydroxybutyrate may provide a feasible strategy to treat the intellectual disability seen in Kabuki syndrome and related disorders.

show abstract

Section: Discussionmentioning

confidence: 99%

A ketogenic diet rescues hippocampal memory defects in a mouse model of Kabuki syndrome

Benjamin

Pilarowski

Carosso

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

120

View full text Add to dashboard Cite

show abstract

“…During the last years, owing to the extensive optimization and standardization, gene expression microarray has become a leading analytical technology in Nutrigenomics research and is expected to play also a crucial role in the emerging Foodomics field (Herrero et al 2012) for the investigation of the interactions between nutrients and other bioactive food compounds and genes (García-Cañas et al 2010). High-density microarrays are widely used for comprehensive gene expression studies in part due to their great transcriptome coverage.…”

Section: Introductionmentioning

confidence: 99%

Effect of rosemary polyphenols on human colon cancer cells: transcriptomic profiling and functional enrichment analysis

et al. 2012

View full text Add to dashboard Cite

In this work, the effect of rosemary extracts rich on polyphenols obtained using pressurized fluids was investigated on the gene expression of human SW480 and HT29 colon cancer cells. The application of transcriptomic profiling and functional enrichment analysis was done via two computational approaches, Ingenuity Pathway Analysis and Gene Set Enrichment Analysis. These two approaches were used for functional enrichment analysis as a previous step for a reliable interpretation of the data obtained from microarray analysis. Reverse transcription quantitative-PCR was used to confirm relative changes in mRNA levels of selected genes from microarrays. The selection of genes was based on their expression change, adjusted p value, and known biological function. According to genome-wide transcriptomics analysis, rosemary polyphenols altered the expression of *4 % of the genes covered by the Affymetrix Human Gene 1.0ST chip in both colon cancer cells. However, only *18 % of the differentially expressed genes were common to both cell lines, indicating markedly different expression profiles in response to the treatment. Differences in induction of G2/ M arrest observed by rosemary polyphenols in the two colon adenocarcinoma cell lines suggest that the extract may be differentially effective against tumors with specific mutational pattern. From our results, it is also concluded that rosemary polyphenols induced a low degree of apoptosis indicating that other multiple signaling pathways may contribute to colon cancer cell death.

show abstract

“…Gene expression analysis offers the opportunity to identify a particular environmental effect on biological pathways as well as how this regulation is potentially altered in the development of certain diseases [19]. Recently, peripheral blood leukocytes were suggested as a surrogate tissue to substitute for traditional tissue specimens that are not easily accessible [20,21].…”

Section: Discussionmentioning

confidence: 99%

Osteochondrosis-Related Gene Expression in Equine Leukocytes Differs among Affected Joints in Foals

Serteyn

Piquemal²,

Mendoza

et al. 2014

J Mol Biomark Diagn

View full text Add to dashboard Cite

Osteochondrosis (OC) is a developmental disease in horses with a significant impact on the horse's welfare and performance. Previously, differentially expressed genes in leukocytes of OC-affected have been identified and were differentially expressed in horses of different ages when compared to their age-matched controls.As the time course of the development of OC lesions seems to be joint dependent,the aim of this study is to compare in young OCaffected horses (between 8 to 12 months), the different expression of selected genes depending the joints involved.The expression of OC-related genes were analysed by rt-PCR and subsequent statistical analysis (ΔΔCT) in the leukocytes of 30 Belgian Warmblood horses aged between 8 to 12 months divided in groups depending the affected joints (fetlock, hock and stifle).In the three groups, expression of ApoB-3G, MGAT4A, B4GALT6 and PRKCG genes were significantly higher in the OC-affected foals compared to the healthy foals. Based on the profiles of expression ofApoB-3G, Dsh1/Dvl1, Foxl1, Hp, ISG15, Mark2, PPR2A, RUSC2 and WASH1 genes,the localization of the disease can be determined: expression levels of ApoB3G, WASH1 and FOXl1 to identify fetlock, ApoB3G, PPR2A to identify OC-development in the hock and ApoB3G, Dsh1/Dvl1, WASH1, PPP2R1A and Mark2 geneto identify OC-development in the stifle. However at this moment, the rt-PCR analysis of the identified genes as biomarkers gives only diagnostic information. For the future, the profile of expression of these genes could give also some predictive information on the evolution of the disease such as remission or permanent OC-lesions.

show abstract

Advances in Nutrigenomics research: Novel and future analytical approaches to investigate the biological activity of natural compounds and food functions

Cited by 104 publications

References 188 publications

A ketogenic diet rescues hippocampal memory defects in a mouse model of Kabuki syndrome

A ketogenic diet rescues hippocampal memory defects in a mouse model of Kabuki syndrome

Effect of rosemary polyphenols on human colon cancer cells: transcriptomic profiling and functional enrichment analysis

Osteochondrosis-Related Gene Expression in Equine Leukocytes Differs among Affected Joints in Foals

Contact Info

Product

Resources

About