Advances in quantitative UV–visible spectroscopy for clinical and pre-clinical application in cancer

Brown, J. Quincy; Vishwanath, Karthik; Palmer, Gregory M.; Ramanujam, Nirmala

doi:10.1016/j.copbio.2009.02.004

Cited by 129 publications

(86 citation statements)

References 93 publications

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“…The idea of information modulation by attenuation has been proposed before [11]. The fact that cells in different metabolic states have different cellular components and different absorption spectra is well known and described in the literature [47][48][49]. We will not discuss this work further.…”

Section: The Mediummentioning

confidence: 99%

Light based cellular interactions: hypotheses and perspectives

Laager¹

2015

Front. Phys.

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This work investigates the theoretical possibility of interactions between cells via light. We first take a brief look at the previous research done in the past to have a better understanding of the field and the origins of the concept of cellular interactions. Then we identify the different elements essential for interactions between two parties. We then compare the required elements with the known and studied elements and characteristics which are well defined in biology, chemistry and physics. This way we are able to set up four postulates required for cell interactions: I. A signal is present and subject to secondary modulation by the emitter cells. II. There is a plastic information medium that reacts directly to the metabolic state of the emitter and therefore carries information about the emitter. III. An optical signal can be perceived by cells on a molecular level by a multitude of different receptors. IV. The information can in theory be processed by cells and metabolic changes in reaction to the signals can be observed. We demonstrate that all required elements have been observed. Most of them have important and well-known roles in cells. Therefore, we suggest that our hypothetical model is a good explanation for light based cellular interactions.

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Section: The Mediummentioning

confidence: 99%

Light based cellular interactions: hypotheses and perspectives

Laager¹

2015

Front. Phys.

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“…For that purpose, it were used several phantoms representative of the changes that occur in the GI tissues during cancer progression, and the fabricated optical filters, placed in front of a UV-Vis-NIR spectrophotometer (Shimadzu UV 3101PC) and a spectrofluorometer (SPEX ® FluoroLog ® 2) detectors. This procedure allows determining the accuracy of the filters to extract the signals at all the relevant spectral bands (350 nm to 750 nm), even with the obtained deviations concerning the filters performance in terms of maximum transmission peak and FWHM (previous discussed in section 5).…”

Section: Diffuse Reflectance and Fluorescence Signals Measurements Wimentioning

confidence: 99%

“…Spectroscopic techniques, specifically diffuse reflectance and fluorescence spectroscopy, can considerably improve the ability to detect GI dysplasia, since they have exquisite sensitivity to some cancer biomarkers present on the tissues [2][3][4][5]. As a result, small changes in those biomarkers concentration, due to cancer progression, can be detected by measuring the diffuse reflectance and fluorescence signals of a GI tissue.…”

Section: Introductionmentioning

confidence: 99%

Design and fabrication of SiO_2/TiO_2 and MgO/TiO_2 based high selective optical filters for diffuse reflectance and fluorescence signals extraction

Pimenta

Freitas

Miranda

et al. 2015

Biomed. Opt. Express

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This paper presents the design, optimization and fabrication of 16 MgO/TiO 2 and SiO 2 /TiO 2 based high selective narrow bandpass optical filters. Their performance to extract diffuse reflectance and fluorescence signals from gastrointestinal tissue phantoms was successfully evaluated. The obtained results prove their feasibility to correctly extract those spectroscopic signals, through a Spearman's rank correlation test (Spearman's correlation coefficient higher than 0.981) performed between the original spectra and the ones obtained using those 16 fabricated optical filters. These results are an important step for the implementation of a miniaturized, low-cost and minimal invasive microsystem that could help in the detection of gastrointestinal dysplasia.

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“…simplicity, ease of operation and convenience, UV-VIS spectrophotometry is a frequently used qualitative and quantitative analytical method in the field of biotechnology [1][2][3][4][5], but also in liquid chromatography [4][5][6][7][8][9][10][11][12][13][14][15][16]. However, besides their advantages, UV-VIS detectors have some drawbacks as well.…”

Section: Introductionmentioning

confidence: 99%