Advances in recombinant protein expression for use in pharmaceutical research

Assenberg, René; Wan, Paul; Geisse, Sabine; Mayr, Lorenz M.

doi:10.1016/j.sbi.2013.03.008

Cited by 159 publications

(85 citation statements)

References 130 publications

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“…We all know that many targets of pharmaceutical research, such as the structure and function of target protein, or even the signal transduction pathway of target proteins are already known. 3 But for anti-plant virus agent research, only very few molecular targets are investigated and can be used in agrochemical design and discovering, 4 which on the other hand increases the difficulty of discovery of antiviral molecules for plants. Therefore, it's a challenge for the development of novel, effective, and environmentally safe antiviral agent.…”

Section: Introductionmentioning

confidence: 99%

Antiviral activity and interaction mechanisms study of novel glucopyranoside derivatives

Chen

et al. 2015

Bioorganic & Medicinal Chemistry Letters

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Section: Introductionmentioning

confidence: 99%

Antiviral activity and interaction mechanisms study of novel glucopyranoside derivatives

Chen

et al. 2015

Bioorganic & Medicinal Chemistry Letters

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“…[91] Challenging recombinant targets may also be expressed in eucaryotic species to exploit their more sophisticated folding or secretion apparatus, or to access their native post-translational modification machineries. [92] Yeasts combine the simplicity, low cost, ease of manipulation and short generation time of a unicellular organism with the ability to carry complex post-translational modifications, [93] including human-like protein glycosylation in engineered strains. [94] Saccharomyces cerevisiae and Pichia pastoris are the best characterized species, with glycoengineered strains of the latter shown to be capable of producing recombinant anti-human epidermal growth factor receptor 2 (HER2) antibodies similar to the FDA-approved and mammalian cellproduced trastuzumab used to treat metastatic breast cancer.…”

Section: Expression Systems For Recombinant Productsmentioning

confidence: 99%

Adding Functions to Biomaterial Surfaces through Protein Incorporation

et al. 2016

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The concept of biomaterials has evolved from one of inert mechanical supports with a long-term, biologically inactive role in the body into complex matrices that exhibit selective cell binding, promote proliferation and matrix production, and may ultimately become replaced by newly generated tissues in vivo. Functionalization of material surfaces with biomolecules is critical to their ability to evade immunorecognition, interact productively with surrounding tissues and extracellular matrix, and avoid bacterial colonization. Antibody molecules and their derived fragments are commonly immobilized on materials to mediate coating with specific cell types in fields such as stent endothelialization and drug delivery. The incorporation of growth factors into biomaterials has found application in promoting and accelerating bone formation in osteogenerative and related applications. Peptides and extracellular matrix proteins can impart biomolecule- and cell-specificities to materials while antimicrobial peptides have found roles in preventing biofilm formation on devices and implants. In this progress report, we detail developments in the use of diverse proteins and peptides to modify the surfaces of hard biomaterials in vivo and in vitro. Chemical approaches to immobilizing active biomolecules are presented, as well as platform technologies for isolation or generation of natural or synthetic molecules suitable for biomaterial functionalization.

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“…The most popular eukaryotic expression system used in structural biology are insect cells, as revealed by analysis of entries to the RCSB's protein databank (Research collaboratory for structural bioinformatics; http://www.rcsb.org/pdb/). For a review, see Assenberg, Wan, Geisse, & Mayr (2013). The major advantage of insect cells is their ability to produce large amounts of properly folded cytosolic proteins and protein complexes, because they possess similar chaperones and folding cofactors as human cells do.…”

Section: Comparison To Other Expression Systemsmentioning

confidence: 99%