Advances in three-dimensional rapid prototyping of microfluidic devices for biological applications

O'Neill, Paul F.; Azouz, Aymen Ben; Vázquez, Mercedes; Liu, J.; Marczak, Steven P.; Slouka, Zdeněk; Chang, Hsueh‐Chia; Diamond, Dermot; Brabazon, Dermot

doi:10.1063/1.4898632

Cited by 129 publications

(101 citation statements)

References 53 publications

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“…181 Instrumentation should ideally be also reconfigurable so that it can easily accommodate the integrated modular platform. 134,135,173 Future precision medicine will heavily depend on the ability to perform frequent liquid biopsies, and in general, on profiling various nucleic acids in serum and other physiological samples for screening, diagnostic, and prognostic applications in cancer and other diseases.…”

Section: Discussionmentioning

confidence: 99%

Future microfluidic and nanofluidic modular platforms for nucleic acid liquid biopsy in precision medicine

et al. 2016

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Nucleic acid biomarkers have enormous potential in non-invasive diagnostics and disease management. In medical research and in the near future in the clinics, there is a great demand for accurate miRNA, mRNA, and ctDNA identification and profiling. They may lead to screening of early stage cancer that is not detectable by tissue biopsy or imaging. Moreover, because their cost is low and they are non-invasive, they can become a regular screening test during annual checkups or allow a dynamic treatment program that adjusts its drug and dosage frequently. We briefly review a few existing viral and endogenous RNA assays that have been approved by the Federal Drug Administration. These tests are based on the main nucleic acid detection technologies, namely, quantitative reverse transcription polymerase chain reaction (PCR), microarrays, and next-generation sequencing. Several of the challenges that these three technologies still face regarding the quantitative measurement of a panel of nucleic acids are outlined. Finally, we review a cluster of microfluidic technologies from our group with potential for point-of-care nucleic acid quantification without nucleic acid amplification, designed to overcome specific limitations of current technologies. We suggest that integration of these technologies in a modular design can offer a low-cost, robust, and yet sensitive/selective platform for a variety of precision medicine applications.

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Section: Discussionmentioning

confidence: 99%

Future microfluidic and nanofluidic modular platforms for nucleic acid liquid biopsy in precision medicine

et al. 2016

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“…3D-printed templates also allow for accelerated testing and modification of device designs; modifications can be added to the template file and printed out immediately (Gross et al 2014;O'Neill et al 2014). MacDonald, et al (2002) have demonstrated a method to create microfluidic devices in PDMS using solid object printing.…”

Section: Introductionmentioning

confidence: 99%

Developing a Protocol for Creating Microfluidic Devices with a 3D Printer

Collette

Novak

Rosen

et al. 2017

IJURCA

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This work has undergone a double-blind review by a minimum of two faculty members from institutions of higher learning from around the world. The faculty reviewers have expertise in disciplines closely related to those represented by this work. If possible, the work was also reviewed by undergraduates in collaboration with the faculty reviewers. AbstractMicrofluidics devices have high importance in fields such as bioanalysis because these devices have the ability to manipulate small volumes of fluid, typically ranging from microliters to picoliters. Small samples of fluids can be quickly and easily tested using reactions performed with complex microfluidic devices. Many methods have been previously developed to create these devices, including traditional nano-lithography techniques borrowed from the field of microelectronics. However, these traditional techniques are cost-prohibitive for many small-scale laboratories. This research explores a relatively low-cost technique using a 3D printed master, which is used as a template for the fabrication of polydimethylsiloxane (PDMS) microfluidic devices. The masters are designed using computer aided design (CAD) software and can be printed and modified relatively quickly. We have developed a protocol for creating simple microfluidic devices using a 3D printer and PDMS adhered to glass. We have also explored methods to overcome the size-limits of the 3D-printed master templates by using shrinkable polymers and modified channel geometries to create a flow-focusing channel. This relatively simple and lower-cost technique can now be scaled to more complicated device designs and applications.

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“…The overall mechanism of these forces in 2D is straightforward. Alternatively, fabrication of 3-D microfluidic devices has been realized with 3-D printing technologies and direct internal 3-D laser writing methods, which was critically reviewed by O'Neill et al; 23 however, it is difficult to employ these 3-D fabrication technologies to embed electrodes in the microfluidic devices, that means, the electrokinetic forces are difficult to be employed to manipulate the particles in 3 dimensions.…”

Section: Introductionmentioning

confidence: 99%

Development of three-dimensional integrated microchannel-electrode system to understand the particles' movement with electrokinetics

et al. 2016

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An optical transparent 3-D Integrated Microchannel-Electrode System (3-DIMES) has been developed to understand the particles' movement with electrokinetics in the microchannel. In this system, 40 multilayered electrodes are embedded at the 2 opposite sides along the 5 square cross-sections of the microchannel by using Micro Electro-Mechanical Systems technology in order to achieve the optical transparency at the other 2 opposite sides. The concept of the 3-DIMES is that the particles are driven by electrokinetic forces which are dielectrophoretic force, thermal buoyancy, electrothermal force, and electroosmotic force in a three-dimensional scope by selecting the excitation multilayered electrodes. As a first step to understand the particles' movement driven by electrokinetic forces in high conductive fluid (phosphate buffer saline (PBS)) with the 3-DIMES, the velocities of particles' movement with one pair of the electrodes are measured three dimensionally by Particle Image Velocimetry technique in PBS; meanwhile, low conductive fluid (deionized water) is used as a reference. Then, the particles' movement driven by the electrokinetic forces is discussed theoretically to estimate dominant forces exerting on the particles. Finally, from the theoretical estimation, the particles' movement mainly results from the dominant forces which are thermal buoyancy and electrothermal force, while the velocity vortex formed at the 2 edges of the electrodes is because of the electroosmotic force. The conclusions suggest that the 3-DIMES with PBS as high conductive fluid helps to understand the threedimensional advantageous flow structures for cell manipulation in biomedical applications. V C 2016 AIP Publishing LLC. [http://dx

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Advances in three-dimensional rapid prototyping of microfluidic devices for biological applications

Cited by 129 publications

References 53 publications

Future microfluidic and nanofluidic modular platforms for nucleic acid liquid biopsy in precision medicine

Future microfluidic and nanofluidic modular platforms for nucleic acid liquid biopsy in precision medicine

Developing a Protocol for Creating Microfluidic Devices with a 3D Printer

Development of three-dimensional integrated microchannel-electrode system to understand the particles' movement with electrokinetics

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