Advances in Ultra-small Fluorescence Nanoprobes for Detection of Metal Ions, Drugs, Pesticides and Biomarkers

In Situ Microscopic Studies on the Interaction of Multi-Principal Element Nanoparticles and Bacteria

“…For future quantitative and statistical analysis, ultrasmall fluorescence nanoprobes can be utilized for the detection of released metal ions in the bacterium cytoplasm. 53 To quantify the released multiple metal ions inside the bacterium cytoplasm, size exclusion chromatography coupled with the inductively coupled plasma-mass spectroscopy (SEC-ICP-MS) technique should be utilized in future studies. 54 In addition to the existing in situ GLC STEM approach, it will be interesting to study the interaction between multielement nanoparticles and bacteria at two-dimensional (2D) and three-dimensional (3D) microscopic imaging levels using ultramicrotomy and laser ablation inductively coupled plasma mass spectroscopy (LA-ICP-MS) techniques, respectively.…”

“…Even though the in situ GLC-STEM approach provides nanoscale insights of bacteria and metal nanoparticles interactions, we would like to highlight that considering the delicacy and beam sensitivity of the specimens, this approach is mainly suitable for qualitative studies. For future quantitative and statistical analysis, ultrasmall fluorescence nanoprobes can be utilized for the detection of released metal ions in the bacterium cytoplasm . To quantify the released multiple metal ions inside the bacterium cytoplasm, size exclusion chromatography coupled with the inductively coupled plasma-mass spectroscopy (SEC-ICP-MS) technique should be utilized in future studies .…”

Synthesis of copper nanoclusters from Bacopa monnieri leaves for fluorescence sensing of dichlorvos

“…Importantly, metal NCs are often utilized as biocompatible probes for bioimaging biological and microbial species with outstanding resolutions because of their unique properties such as molecular properties, tunable emission spectra, good quantum yield (QY), and biocompatibility [4][5][6][7]. As a result, significant efforts have focused on the introduction of metal NCs as probes for different research fields including ion sensing [8,9], biosensing [10,11], bioimaging [12], drug delivery [13,14], and as antibacterial agents [15,16]. Recently, a few metal NCs, such as AuNCs, AgNCS, CuNCs and MoNCs, have been reported as superior fluorescence sensors for molecular assays and bioimaging applications [6,8].…”

“…As a result, significant efforts have focused on the introduction of metal NCs as probes for different research fields including ion sensing [8,9], biosensing [10,11], bioimaging [12], drug delivery [13,14], and as antibacterial agents [15,16]. Recently, a few metal NCs, such as AuNCs, AgNCS, CuNCs and MoNCs, have been reported as superior fluorescence sensors for molecular assays and bioimaging applications [6,8]. Among the above metal NCs, CuNCs are often selected as an alternative fluorescence sensor for the analysis of numerous trace-level analytes and imaging of cells because of several features such as being inexpensive precursors, with moderative QY, megaStokes shifting, tunable emissions and biocompatibility [17].…”

“…Therefore, CuNCs act as promising probes for assaying trace-level analytes with improved analytical features [6,8]. In order to obtain high-quality atomically precise CuNCs, several synthetic strategies such as microwave-assisted [18], electrochemical [19], photoreductive [20], ligand-assisted [21] and chemical methods using proteins [22][23][24], DNA [25] and polymers [26] as templates have been successfully demonstrated the fabrication of atomically ordered fluorescent CuNCs.…”