Advances in Understanding Begomovirus Satellites

Zhou, Xueping

doi:10.1146/annurev-phyto-082712-102234

Cited by 374 publications

(297 citation statements)

References 148 publications

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“…Begomoviruses can be subdivided into two major groups: bipartite viruses, those with a genome composed of two ~2.6 kb DNA components (referred to as DNA-A and DNA-B), which are prevalent in the New World (NW, e.g., southern states of the United States of America (USA), Mexico, the Caribbean and Central and South America); and monopartite viruses, those with a genome composed of a single ~2.9 kb genomic DNA, which are prevalent in the Old World (OW, e.g., Europe, Africa, Asia and Australia). Many monopartite begomoviruses are associated with satellite DNAs (alphasatellites and betasatellites), some of which enhance pathogenicity (Zhou, 2013). The bipartite and monopartite begomoviruses reflect an ancestral division that possibly occurred before continental drift.…”

Section: Emergence Of Whitefly-transmitted Geminiviruses (Begomoviruses)mentioning

confidence: 99%

A review of geminivirus diseases in vegetables and other crops in Brazil: current status and approaches for management

2016

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Insect-transmitted viruses cause some of the most damaging and economically important diseases of crop plants, especially in tropical and subtropical regions. The geminiviruses, a group of single-stranded DNA viruses with unique twined icosahedral virus particles, are responsible for many of these diseases. Of particular importance are the geminiviruses transmitted by whiteflies (Bemisia tabaci), which are in the genus Begomovirus. This is the largest genus of plant viruses (in terms of number of species), and some are responsible for devastating diseases in vegetable and fiber crops throughout the world. This review presents the current understanding of begomoviruses, the diseases they cause, and approaches for disease management. An emphasis is placed on the most important begomovirus diseases in Brazil, which are those that affect common bean and tomato. However, because of the wide host range of the vector and the genetic flexibility of begomoviruses, these diseases pose a threat to many other crops. Therefore, the current situation with begomoviruses that infect okra, pepper, potato, sweet potato and soybean in Brazil is also discussed.

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Section: Emergence Of Whitefly-transmitted Geminiviruses (Begomoviruses)mentioning

confidence: 99%

A review of geminivirus diseases in vegetables and other crops in Brazil: current status and approaches for management

2016

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“…The Geminiviridae family encompasses a large number of plant viruses with circular and single-strand DNA genomes that are encapsidated in twinned icosahedral particles consisting of two incomplete icosahedra (T = 1) that are approximately 22 × 38 nm (Zhou, 2013). On the basis of their genome organization and biological properties, geminiviruses are divided into the Received;August 29, 2016.…”

Section: Introductionmentioning

confidence: 99%

Highly Efficient Agroinoculation Method for Tomato Plants with <i>Tomato Yellow Leaf Curl Kanchanaburi Virus</i>

Koeda¹,

Homma²,

Tanaka³

et al. 2017

The Hortic J

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Tomato yellow leaf curl disease caused by begomoviruses is a serious threat to tomato (Solanum lycopersicum L.) production. If begomoviruses, transmitted by whitefly (Bemisia tabaci), infect tomato plants during early growth, production can be almost entirely lost. Tomato yellow leaf curl Kanchanaburi virus (TYLCKaV), a bipartite Begomovirus, is emerging as an important threat to solanaceous crop production in Southeast Asia. The lack of mechanical transmission of some begomoviruses is a major experimental constraint. In this study, an agroinoculation method using TYLCKaV in tomato plants was established. Partial tandem repeats of TYLCKaV DNA A and DNA B were constructed and cloned to a binary pGreenII vector, and their infectivity was tested. Co-inoculation of TYLCKaV DNA A and DNA B to Nicotiana benthamiana L. produced typical begomoviral symptoms, and both of the viral DNA components accumulated in the upper uninoculated leaves, suggesting systemic infection of TYLCKaV. Two agroinoculation methods were conducted on tomatoes. First, excised sections of tomato shoots were agroinoculated with a soaking procedure. Although two Agrobacterium tumefaciens strains were tested, approximately 40% of inoculated plants only showed viral symptoms for EHA105. Second, agrobacterium from a cultured petri dish was directly inoculated with a colony inoculation procedure. When EHA105 was used, approximately 92% of inoculated plants showed viral symptoms. Sequencing the recovered viral DNA from the upper uninoculated leaf clarified that TYLCKaV had successfully infected the tomato plants. The colony inoculation procedure is labor-saving, and viral symptoms develop in susceptible tomato plants within approximately a month from sowing the seeds. This method could contribute to simple and speedy evaluation of TYLCKaV resistance of tomato plants.

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“…At this stage, the presence of additional genomic components of MMDaV, as well as of DNA satellites similar to those reported in some begomoviruses with monopartite genomes (Zhou, 2013), could not be excluded. To gain insights into this, rolling-circle amplification (RCA) followed by RFLP analyses (Haible et al, 2006;InoueNagata et al, 2004) was performed using DNA preparations from AK1 and a healthy mulberry control.…”

Section: Resultsmentioning

confidence: 90%

Identification and molecular characterization of a novel monopartite geminivirus associated with mulberry mosaic dwarf disease

Navarro

Zhang

et al. 2015

Journal of General Virology

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High-throughput sequencing of small RNAs allowed the identification of a novel DNA virus in a Chinese mulberry tree affected by a disease showing mosaic and dwarfing symptoms. Rollingcircle amplification and PCR with specific primers, followed by sequencing of eleven independent full-length clones, showed that this virus has a monopartite circular DNA genome (,2.95 kb) containing ORFs in both polarity strands, as reported previously for geminiviruses. A field survey showed the close association of the virus with diseased mulberries, so we tentatively named the virus mulberry mosaic dwarf-associated virus (MMDaV). The MMDaV genome codes for five and two putative proteins in the virion-sense and in the complementarysense strands, respectively. Although three MMDaV virion-sense putative proteins did not share sequence homology with any protein in the databases, functional domains [coiled-coil and transmembrane (TM) domains] were identified in two of them. In addition, the protein containing a TM domain was encoded by an ORF located in a similar genomic position in MMDaV and in several other geminiviruses. As reported for members of the genera Mastrevirus and Becurtovirus, MMDaV replication-associated proteins are expressed through the alternative splicing of an intron, which was shown to be functional in vivo. A similar intron was found in the genome of citrus chlorotic dwarf-associated virus (CCDaV), a divergent geminivirus found recently in citrus. On the basis of pairwise comparisons and phylogenetic analyses, CCDaV and MMDaV appear to be closely related to each other, thus supporting their inclusion in a putative novel genus in the family Geminiviridae.

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Advances in Understanding Begomovirus Satellites

Cited by 374 publications

References 148 publications

A review of geminivirus diseases in vegetables and other crops in Brazil: current status and approaches for management

A review of geminivirus diseases in vegetables and other crops in Brazil: current status and approaches for management

Highly Efficient Agroinoculation Method for Tomato Plants with <i>Tomato Yellow Leaf Curl Kanchanaburi Virus</i>

Identification and molecular characterization of a novel monopartite geminivirus associated with mulberry mosaic dwarf disease

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