Advances in wound dressings and cultured skin substitutes

Yoshimitsu, Kengo

doi:10.1007/bf02480051

Cited by 13 publications

(9 citation statements)

References 64 publications

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“…2 In this respect, the application of synthetic membranes recently has been considered to be very prospective, as their chemical composition and mechanical properties can be easily modified according to specific needs. [3][4][5][6] The first tissueengineered organ, processed from the lab for the first accepted patients' care, was skin. 7 Currently, there is a huge number of patients with deep burns or nonhealing skin ulcers, and many of them would benefit from a large-scale production of bioartificial skin.…”

Section: Introductionmentioning

confidence: 99%

“…8 The common tissue engineering technologies are based on the creation of living stroma of fibroblasts and/or keratinocytes, which have been cultured to obtain sufficient cell numbers. [9][10][11] Conventional monolayer cell culture systems, however, do not provide the normal cellular environment for large-scale production and may enhance dedifferentiation of cells. 12,13 Moreover, under such conditions the cells may suffer from insufficient delivery of oxygen and nutrients.…”

Section: Introductionmentioning

confidence: 99%

“…12,13 Moreover, under such conditions the cells may suffer from insufficient delivery of oxygen and nutrients. Furthermore, the mechanical stability of these skin substitutes, as well as their method of application to the wound, suf-fer from technical problems, 11 which still are not completely resolved. Thus, the implementation of a bioengineered skin consisting of cultured dermal cells on polymer membranes may be considered as one step toward the treatment of wounds and for the substitution of large areas of skin.…”

Section: Introductionmentioning

confidence: 99%

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Interaction of human skin fibroblasts with moderate wettable polyacrylonitrile–copolymer membranes

Groth¹,

Seifert²,

Malsch³

et al. 2002

J. Biomed. Mater. Res.

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The development of a bioartificial skin is a step toward the treatment of patients with deep burns or nonhealing skin ulcers. One possible approach is based on growing dermal cells on membranes to obtain appropriate living cellular stroma (sheets) to cover the wound. New membrane-forming copolymers were synthesized, based on acrylonitrile (AN) copolymerization with hydrophilic N-vinylpyrrolidone (NVP) monomer, in different percentage ratios, such as 5, 20, and 30% w/w, and with two other relatively high polar comonomers--namely, sodium 2-methyl-2-propene-1-sulfonic acid (NaMAS) and aminoethylmethacrylate (AeMA). All these copolymers were characterized for their bulk composition and number average molecular weight, and used to prepare ultrafiltration membranes. Water contact angles and water uptake were estimated to characterize the wettability and scanning force microscopy to visualize the morphology of the resulting polymer surface. Cytotoxicity was estimated according to the international standard regulations, and the materials were found to be nontoxic. The interaction of the membranes with human skin fibroblasts was investigated considering that these cells are among the first to colonize membranes upon implantation or with prolonged external contact. The overall cell morphology, formation of focal adhesion contacts, and cell proliferation were estimated to characterize the cell material interactions. It was found that the pure polyacrylonitrile homopolymer (PAN) membrane provides excellent conditions for seeding with fibroblasts, comparable only to a copolymer containing AeMA. In contrast, the presence of NaMAS with acidic ionic groups decreased both the attachment and proliferation of fibroblasts. Low content of NVP in the copolymer, up to about 5%, still enabled good attachment and spreading of cells, as well as subsequent proliferation of fibroblasts, but higher ratios of 20 and 30% resulted in a significant decrease of these cellular activities.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Interaction of human skin fibroblasts with moderate wettable polyacrylonitrile–copolymer membranes

Groth¹,

Seifert²,

Malsch³

et al. 2002

J. Biomed. Mater. Res.

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“…24 If only the cell attachment function may be considered, then β-glucan and HA are not suitable for manufacturing the scaffold, because polysaccharides, such as β-glucan and HA, have been reported to be poor substrates for cell attachment. 25 However, it is expected that the addition of β-glucan and HA may have advantages for the mechanical properties, for the morphologies, and for its effect on certain bio-active wound healing properties.…”

Section: Resultsmentioning

confidence: 99%

“…Several studies have indicated that the presence of fibroblasts in a dermal equivalent stimulates epidermal differentiation. 25,29,30 It is assumed that the presence of fibroblasts in a dermal equivalent accelerate the healing process by reducing the time needed for the fibroblasts to invade the wound tissue and by early synthesis of new skin tissue, because the fibroblasts on the artificial dermis can release biologicallyactive substances, such as cytokines. These cytokines promote healing by stimulating the production of components in the basement membrane, so preventing dehydration and increasing inflammation, and promoting the formation of granulation tissue.…”

Section: Resultsmentioning

confidence: 99%

Artificial dermis composed of gelatin, hyaluronic acid and (1→3),(1→6)-β-glucan

et al. 2003

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Porous scaffolds composed of gelatin and polysaccharides such as hyaluronic acid and β-glucan were prepared by using the freeze-drying method after cross-linking with 1-ethyl-(3-3-dimethylaminopropyl) carbodiimide hydrochloride (EDC). The scaffold had an inter-connected pore structure with the sufficient pore size for use as a support for the growth of fibroblasts. Results for the contact angle and cell attachment confirmed that high gelatin content in a mixture was suitable for cellular attachment and distribution in two-or three-dimensional fibroblast cultures. However, the addition of polysaccharides aroused the synergistic effects of morphologic and mechanical property of gelatin-based scaffolds. To prepare the artificial dermis for the wound dressing to mimic the normal human dermal skin, fibroblasts were isolated from a childs foreskin, and cultured in gelatin-based scaffolds. An in vivo study showed that the artificial dermis containing the fibroblasts enhanced the wound healing rate and re-epithelialization of a full-thickness skin defect rather than the acellular scaffold after one week.

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