Advancing bone tissue engineering one layer at a time: a layer-by-layer assembly approach to 3D bone scaffold materials

Sahebalzamani, Mohammadali; Ziminska, Monika; McCarthy, Helen; Levingstone, Tanya J.; Dunne, Nicholas; Hamilton, Andrew

doi:10.1039/d1bm01756j

Cited by 34 publications

(27 citation statements)

References 218 publications

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“…The PEM provides a highly tunable surface fulfilling these demands. The negative charge of the coated surface attracts cells and makes them easy to adhere; in addition, cell adhesion and the necessary cell motility are more active on harder surfaces than on softer ones ( Sahebalzamani et al, 2022 ). Therefore, a PEM-Col surface has been chosen for proof of this strategy.…”

Section: Resultsmentioning

confidence: 99%

Encapsulated vaterite-calcite CaCO3 particles loaded with Mg2+ and Cu2+ ions with sustained release promoting osteogenesis and angiogenesis

Fan

Körte

Rudt

et al. 2022

Front. Bioeng. Biotechnol.

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Bioactive cations, including calcium, copper and magnesium, have shown the potential to become the alternative to protein growth factor-based therapeutics for bone healing. Ion substitutions are less costly, more stable, and more effective at low concentrations. Although they have been shown to be effective in providing bone grafts with more biological functions, the precise control of ion release kinetics is still a challenge. Moreover, the synergistic effect of three or more metal ions on bone regeneration has rarely been studied. In this study, vaterite-calcite CaCO3 particles were loaded with copper (Cu2+) and magnesium (Mg2+). The polyelectrolyte multilayer (PEM) was deposited on CaCuMg-CO3 particles via layer-by-layer technique to further improve the stability and biocompatibility of the particles and to enable controlled release of multiple metal ions. The PEM coated microcapsules were successfully combined with collagen at the outmost layer, providing a further stimulating microenvironment for bone regeneration. The in vitro release studies showed remarkably stable release of Cu2+ in 2 months without initial burst release. Mg2+ was released in relatively low concentration in the first 7 days. Cell culture studies showed that CaCuMg-PEM-Col microcapsules stimulated cell proliferation, extracellular maturation and mineralization more effectively than blank control and other microcapsules without collagen adsorption (Ca-PEM, CaCu-PEM, CaMg-PEM, CaCuMg-PEM). In addition, the CaCuMg-PEM-Col microcapsules showed positive effects on osteogenesis and angiogenesis in gene expression studies. The results indicate that such a functional and controllable delivery system of multiple bioactive ions might be a safer, simpler and more efficient alternative of protein growth factor-based therapeutics for bone regeneration. It also provides an effective method for functionalizing bone grafts for bone tissue engineering.

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Section: Resultsmentioning

confidence: 99%

Encapsulated vaterite-calcite CaCO3 particles loaded with Mg2+ and Cu2+ ions with sustained release promoting osteogenesis and angiogenesis

Fan

Körte

Rudt

et al. 2022

Front. Bioeng. Biotechnol.

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“…In this delivery system, the outer layer degrades before the inner, which results in delivery of different molecules in different phases. This approach has been systematically reviewed by Sahebalzamani et al, and they considered the method to be promising for smart ion delivery in the future . Another strategy for sustainable ion release with great potential is composite gels via electrostatic interaction. , …”

Section: Delivery Of Ions By Biomaterials Carriersmentioning

confidence: 99%

Effects of Zinc, Magnesium, and Iron Ions on Bone Tissue Engineering

Chen

Zhang

Wang

et al. 2022

ACS Biomater. Sci. Eng.

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Large-sized bone defects are a great challenge in clinics and considerably impair the quality of patients’ daily life. Tissue engineering strategies using cells, scaffolds, and bioactive molecules to regulate the microenvironment in bone regeneration is a promising approach. Zinc, magnesium, and iron ions are natural elements in bone tissue and participate in many physiological processes of bone metabolism and therefore have great potential for bone tissue engineering and regeneration. In this review, we performed a systematic analysis on the effects of zinc, magnesium, and iron ions in bone tissue engineering. We focus on the role of these ions in properties of scaffolds (mechanical strength, degradation, osteogenesis, antibacterial properties, etc.). We hope that our summary of the current research achievements and our notifications of potential strategies to improve the effects of zinc, magnesium, and iron ions in scaffolds for bone repair and regeneration will find new inspiration and breakthroughs to inspire future research.

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“…The scaffold material influences the seeded cells and loaded biofactors and acts as a support for tissue/cell regeneration. So, ideally, the scaffold material should be able to provide an appropriate biomechanical support and biochemical environment to promote cell adhesion, migration, proliferation, osteogenic differentiation and angiogenesis by imitating the natural bone extracellular matrix [ 33 , 39 ]. An ideal scaffold material should also exhibit high mechanical properties for load-bearing and proper pore interconnectivity and size for transport of nutrients and oxygen and for balancing mechanical integrity, cell adhesion, infiltration and differentiation.…”

Section: Traditional and Novel Approaches In Bone Tissue Engineeringmentioning

confidence: 99%

“…Controlled hierarchical pore structure with interconnected pore networks is crucial for fabricating bone tissue [ 41 ]. Fabrication of 3D scaffolds providing sufficient mechanical support, interconnected porosity, proper surface topography and degradation rate mimicking bone tissue microenvironment is a great challenge [ 3 , 39 ], particularly when high mechanical strength in the bone repair process is required and increased porosity may reduce scaffold strength [ 8 ]. Furthermore, for biodegradable materials, the biodegradation should be compatible with the growth rate of new bone to achieve a gradual transfer of the loads to the healing bone, providing suitable mechanical support for new bone tissue and matching the rate of bone tissue regeneration [ 39 , 42 ].…”

Section: Traditional and Novel Approaches In Bone Tissue Engineeringmentioning

confidence: 99%

“…Fabrication of 3D scaffolds providing sufficient mechanical support, interconnected porosity, proper surface topography and degradation rate mimicking bone tissue microenvironment is a great challenge [ 3 , 39 ], particularly when high mechanical strength in the bone repair process is required and increased porosity may reduce scaffold strength [ 8 ]. Furthermore, for biodegradable materials, the biodegradation should be compatible with the growth rate of new bone to achieve a gradual transfer of the loads to the healing bone, providing suitable mechanical support for new bone tissue and matching the rate of bone tissue regeneration [ 39 , 42 ]. A clinically applicable scaffold needs to simultaneously possess some specific features such as biocompatibility, biodegradability, osteoconductivity, low immunogenicity and non-infectivity [ 42 ].…”

Section: Traditional and Novel Approaches In Bone Tissue Engineeringmentioning

confidence: 99%

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Novel Approaches and Biomaterials for Bone Tissue Engineering: A Focus on Silk Fibroin

Paladini

Pollini

2022

Materials

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Bone tissue engineering (BTE) represents a multidisciplinary research field involving many aspects of biology, engineering, material science, clinical medicine and genetics to create biological substitutes to promote bone regeneration. The definition of the most appropriate biomaterials and structures for BTE is still a challenge for researchers, aiming at simultaneously combining different features such as tissue generation properties, biocompatibility, porosity and mechanical strength. In this scenario, among the biomaterials for BTE, silk fibroin represents a valuable option for the development of functional devices because of its unique biological properties and the multiple chances of processing. This review article aims at providing the reader with a general overview of the most recent progresses in bone tissue engineering in terms of approaches and materials with a special focus on silk fibroin and the related mechanisms involved in bone regeneration, and presenting interesting results obtained by different research groups, which assessed the great potential of this protein for bone tissue engineering.

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Advancing bone tissue engineering one layer at a time: a layer-by-layer assembly approach to 3D bone scaffold materials

Abstract: The layer-by-layer (LbL) assembly technique has shown excellent potential for tissue engineering applications. The technique is mainly based on electrostatic attraction and involves the sequential adsorption of oppositely charged electrolyte...

Cited by 34 publications

References 218 publications

Encapsulated vaterite-calcite CaCO3 particles loaded with Mg2+ and Cu2+ ions with sustained release promoting osteogenesis and angiogenesis

Encapsulated vaterite-calcite CaCO3 particles loaded with Mg2+ and Cu2+ ions with sustained release promoting osteogenesis and angiogenesis

Effects of Zinc, Magnesium, and Iron Ions on Bone Tissue Engineering

Novel Approaches and Biomaterials for Bone Tissue Engineering: A Focus on Silk Fibroin

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