Advantages of decellularized bovine pericardial scaffolds compared to glutaraldehyde fixed bovine pericardial patches demonstrated in a 180-day implant ovine study

Botes, Lezelle; Laker, Leana; Dohmen, Pascal M.; Heever, Johannes Jacobus van den; Jordaan, Christiaan Johannes; Lewies, Angélique; Smit, Francis E.

doi:10.1007/s10561-021-09988-8

Cited by 7 publications

(16 citation statements)

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“…This process resulted in more compacted and dense collagen layers when compared to the leaflet and wall tissue in the cryopreserved and decellularized groups (Figures 4G, 5G and 7E,F), which might make the recellularization of the matrix with interstitial cells more difficult [42]. Using a juvenile ovine model, Botes et al (2022) demonstrated that commercial EnCap TMtreated bovine pericardium (not decellularized) had less host cell infiltration than the bovine pericardium processed using our proprietary decellularization process. The EnCap TMtreated tissue showed a loss of collagen fiber integrity, causing the collagen to become densely compacted, contributing to insignificant recipient cell infiltration [54].…”

Section: Discussionmentioning

confidence: 99%

Biomaterials for Tissue Engineering and Regeneration

2023

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Section: Discussionmentioning

confidence: 99%

Biomaterials for Tissue Engineering and Regeneration

2023

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“…However, its use compromises the integrity of collagen fibres and inhibits the infiltration of host cells into the graft. 51 Gamma irradiation sterilization destroys collagen fibres and impairs tissue regeneration. 52 Sterilization using peracetic acid, which is highly acidic and a strong oxidant, can affect the physical and chemical properties of the tissue.…”

Section: Discussionmentioning

confidence: 99%

A survey on the usage of decellularized tissues in orthopaedic clinical trials

et al. 2023

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AimsOrthopaedic surgery requires grafts with sufficient mechanical strength. For this purpose, decellularized tissue is an available option that lacks the complications of autologous tissue. However, it is not widely used in orthopaedic surgeries. This study investigated clinical trials of the use of decellularized tissue grafts in orthopaedic surgery.MethodsUsing the ClinicalTrials.gov (CTG) and the International Clinical Trials Registry Platform (ICTRP) databases, we comprehensively surveyed clinical trials of decellularized tissue use in orthopaedic surgeries registered before 1 September 2022. We evaluated the clinical results, tissue processing methods, and commercial availability of the identified products using academic literature databases and manufacturers’ websites.ResultsWe initially identified 4,402 clinical trials, 27 of which were eligible for inclusion and analysis, including nine shoulder surgery trials, eight knee surgery trials, two ankle surgery trials, two hand surgery trials, and six peripheral nerve graft trials. Nine of the trials were completed. We identified only one product that will be commercially available for use in knee surgery with significant mechanical load resistance. Peracetic acid and gamma irradiation were frequently used for sterilization.ConclusionDespite the demand for decellularized tissue, few decellularized tissue products are currently commercially available, particularly for the knee joint. To be viable in orthopaedic surgery, decellularized tissue must exhibit biocompatibility and mechanical strength, and these requirements are challenging for the clinical application of decellularized tissue. However, the variety of available decellularized products has recently increased. Therefore, decellularized grafts may become a promising option in orthopaedic surgery.Cite this article: Bone Joint Res 2023;12(3):179–188.

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“…Using a juvenile ovine model, Botes et al (2022) demonstrated that commercial EnCap TM- treated bovine pericardium (not decellularized) had less host cell infiltration than the bovine pericardium processed using our proprietary decellularization process. The EnCap TM- treated tissue showed a loss of collagen fiber integrity, causing the collagen to become densely compacted, contributing to insignificant recipient cell infiltration [ 54 ]. The current study showed that even after decellularization, GA fixation led to the collagen layers of the homograft becoming compacted and dense, which might affect cell infiltration.…”

Section: Discussionmentioning

confidence: 99%

Impact of Three Different Processing Techniques on the Strength and Structure of Juvenile Ovine Pulmonary Homografts

et al. 2022

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Homografts are routinely stored by cryopreservation; however, donor cells and remnants contribute to immunogenicity. Although decellularization strategies can address immunogenicity, additional fixation might be required to maintain strength. This study investigated the effect of cryopreservation, decellularization, and decellularization with additional glutaraldhyde fixation on the strength and structure of ovine pulmonary homografts harvested 48 h post-mortem. Cells and cellular remnants were present for the cryopreserved group, while the decellularized groups were acellular. The decellularized group had large interfibrillar spaces in the extracellular matrix with uniform collagen distribution, while the additional fixation led to the collagen network becoming dense and compacted. The collagen of the cryopreserved group was collapsed and appeared disrupted and fractured. There were no significant differences in strength and elasticity between the groups. Compared to cryopreservation, decellularization without fixation can be considered an alternative processing technique to maintain a well-organized collagen matrix and tissue strength of homografts.

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Advantages of decellularized bovine pericardial scaffolds compared to glutaraldehyde fixed bovine pericardial patches demonstrated in a 180-day implant ovine study

Cited by 7 publications

References 35 publications

Biomaterials for Tissue Engineering and Regeneration

Biomaterials for Tissue Engineering and Regeneration

A survey on the usage of decellularized tissues in orthopaedic clinical trials

Impact of Three Different Processing Techniques on the Strength and Structure of Juvenile Ovine Pulmonary Homografts

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