Advantages of time-resolved fluorescent nanobeads compared with fluorescent submicrospheres, quantum dots, and colloidal gold as label in lateral flow assays for detection of ractopamine

Hu, Liming; Luo, Kai; Xia, Jun; Xu, Guowang; Wu, Chenghui; Han, Jiaojiao; Zhang, Gang-Gang; Liu, Miao; Lai, Weihua

doi:10.1016/j.bios.2016.12.030

Cited by 142 publications

(55 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Carboxylated quantum dot nanobeads (QB, 1 uM, w/v, excitation = 365 nm, emission = 610 nm) and quantum dots (QD, 1.0 mg/mL, w/v; carboxylate-modified CdSe/ZnS core/shell nanocrystals with amphiphilic polymer coating; excitation Although many methods based on immune interactions have been developed for the detection of toxic and harmful substances, it is impossible to compare the performance of those methods for identifying the most appropriate approach due to the utilization of distinct antibodies/antigens, markers and the detection conditions. In recent years, only a few reports have used comparative methods under the same conditions [26][27][28][29][30][31]. For instance, Xie et al [27] established flow immunochromatography to detect Escherichia coli O157:H7 in milk, in which fluorescent microspheres and colloidal gold were compared in terms of antibody labeling efficiency, sensitivity, antibody consumption and coefficient of variation.…”

Section: Methodsmentioning

confidence: 99%

“…The TRFN-mAb was prepared based on the procedures described in the previous literature with slight modification [26,30]. Briefly, 5 µL of TRFN was dissolved in 45 µL of activation buffer (50 mM MES (2-Morpholinoethanesulfonic Acid), pH 6.0) and then centrifuged at 20,000× g for 15 min at 4 • C. Subsequently, 40 µL of activation buffer, 5 µL of NHS solution (1 mM) and 5 µL of EDC solution (1 mM) were added to the tube and stirred for 15 min; the solution was centrifuged at 20,000× g for 15 min and the precipitate was resuspended in 25 µL boric acid buffer (40 mM, pH 8.0).…”

Section: Preparation Of Three Labeled Antibody Probesmentioning

confidence: 99%

See 1 more Smart Citation

Comparative Study of Time-Resolved Fluorescent Nanobeads, Quantum Dot Nanobeads and Quantum Dots as Labels in Fluorescence Immunochromatography for Detection of Aflatoxin B1 in Grains

Wang

et al. 2020

Biomolecules

View full text Add to dashboard Cite

Label selection is an essential procedure for improving the sensitivity of fluorescence immunochromatography assays (FICAs). Under optimum conditions, time-resolved fluorescent nanobeads (TRFN), quantum dots nanobeads (QB) and quantum dots (QD)-based immunochromatography assays (TRFN-FICA, QB-FICA and QD-FICA) were systematically and comprehensively compared for the quantitative detection of aflatoxin B 1 (AFB 1 ) in six grains (corn, soybeans, sorghum, wheat, rice and oat). All three FICAs can be applied as rapid, cost-effective and convenient qualitative tools for onsite screening of AFB 1 ; TRFN-FICA exhibits the best performance with the least immune reagent consumption, shortest immunoassay duration and lowest limit of detection (LOD). The LODs for TRFN-FICA, QB-FICA and QD-FICA are 0.04, 0.30 and 0.80 µg kg −1 in six grains, respectively. Recoveries range from 83.64% to 125.61% at fortified concentrations of LOD, 2LOD and 4LOD, with the coefficient of variation less than 10.0%. Analysis of 60 field grain samples by three FICAs is in accordance with that of LC-MS/MS, and TRFN-FICA obtained the best fit. In conclusion, TRFN-FICA is more suitable for quantitative detection of AFB 1 in grains when the above factors are taken into consideration.Biomolecules 2020, 10, 575 2 of 12 To better monitor the threat of AFB 1 contamination, various methods have been developed in the past few decades [9][10][11][12]. Although the results are reliable and accurate, instrumental techniques [13] need expensive equipment and complicated sample pretreatment. Biosensors based on the antibody immunoprobes such as enzyme-linked immunosorbent assay (ELISA) [14] and fluorescence-linked immunosorbent assay (FLISA) [15,16] can achieve quantitative detection with good performance of specificity, sensitivity and simplicity, but the heterogeneous immunoassays require multiwashing procedures and long analysis times. To address the above issues, lateral flow immunochromatography assays have been considered as a promising method for onsite screening of mycotoxins [17][18][19]. Moreover, immunochromatography assays based on fluorescent markers (time-resolved fluorescent nanobeads (TRFN), quantum dot nanobeads (QB) and quantum dots (QD), etc.) have gradually become a popular research field in recent years for their advantages of sensitivity, accuracy, automated detection, shorter detection time, and so on [20][21][22]. Several fluorescence immunochromatography assays for highly sensitive detection of AFB 1 have been reported [20,21,[23][24][25].

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Preparation Of Three Labeled Antibody Probesmentioning

confidence: 99%

Comparative Study of Time-Resolved Fluorescent Nanobeads, Quantum Dot Nanobeads and Quantum Dots as Labels in Fluorescence Immunochromatography for Detection of Aflatoxin B1 in Grains

Wang

et al. 2020

Biomolecules

View full text Add to dashboard Cite

show abstract

“…Exactly 0.1 mL of BSA (10%, w/v) was added to the solution for 30 min. The resulting mixtures were separated by a magnetic field for 5 min, and the precipitate was redissolved in 1 mL of phosphate buffer (0.02 M, pH 7.4) with 1% BSA (w/v), 1% Tween-20 (v/v), 5% sucrose (w/v), 3% trehalose (w/v), and 0.1% sodium azide (w/v) (Hu et al, 2017).…”

Section: Preparation Of the Aumb-mab Probementioning

confidence: 99%

“…The use of fluorescence signals to improve the sensitivity of LFA has recently attracted increased research attention . Fluorescent microspheres (FM) possessing a stable configuration, high fluorescence intensity, multicolour selection, and high safety have been employed in food safety (Hu et al, 2017;Wang et al, 2018) and medical diagnostics. Some research groups (Cong et al, 2015) have even reported a fluorescence quenching-based LFA with a signal "switch-on" mode that can improve the sensitivity of competitive LFA.…”

Section: Introductionmentioning

confidence: 99%

Dual signal insight: field-efficient qualitative/quantitative detection of sulphamethazine in raw milk

Song

Huang

Chen

et al. 2019

Food and Agricultural Immunology

Self Cite

View full text Add to dashboard Cite

A novel lateral flow assay (LFA) was developed by introducing magnetic beads with colloidal gold nanoparticles (AuMB) to LFA test strips for the qualitative and quantitative detection of sulphamethazine (SM 2 ) in raw milk. In the proposed assay, test samples are qualitatively judged yes (positive) or no (negative) on the basis of naked-eye signals from the colloidal gold and fluorescence quenching is used to quantitatively determine the level of SM 2 in positive samples. Results indicated that the threshold concentration for determining whether a test sample is positive or negative is 5 ng/mL. The limit of detection and linear range of the proposed AuMB-LFA for quantitative detection were 0.39 and 0.5-200 ng/mL, respectively, and its recovery for detecting SM 2 in raw milk was 97.4-110.2%. The developed AuMB-LFA provides novel insights into efforts to maintain a balance between the efficient screening of targets in large samples and saving time and money. ARTICLE HISTORY

show abstract

“…The possibilities of using carbon nanoparticles described in Van Amerongen et al [97,98] and Liu et al [99], using the example of salbutamol detection, also showed the advantages of colloidal carbon compared to colloidal gold and nanogold-polyanilinenanogold microspheres. For ractopamine detection, Hu et al [100] showed the advantages of time-resolved fluorescent nanobeads compared with fluorescent submicrospheres, quantum dots, and colloidal gold. Effective integration of palladium nanoparticles and horseradish peroxidase with a 10-fold gain in sensitivity as compared to colloidal gold in the detection of Listeria monocytogenes was described by Tominaga [101].…”

Section: Proper Response For Lfiamentioning

confidence: 99%

Ways to Reach Lower Detection Limits of Lateral Flow Immunoassays

Zherdev¹,

Dzantiev²

2018

Rapid Test - Advances in Design, Format and Diagnostic Applications

View full text Add to dashboard Cite

This chapter considers factors influencing sensitivity of lateral flow immunoassay and modern developments that are focused on reaching lower detection limits. The existing variety of proposed approaches is classified in accordance with the "big five rules" for these assays, including proper sample, receptor, interaction, response, and output. The solutions for rapid extraction of target analytes and preventing negative influence of extractants are considered. Role to antibodies affinity and specificity is characterized. Potential of alternate bioreceptor molecules is discussed. Immunoreactants' compositions, concentrations, and locations on the test strip are characterized as factors determining assay parameters. The existing variety of labels is compared in terms of their optical and alternate registration. Tools to modulate a sequence of analytical reactions and to form aggregates of the detected labels are considered. The discussed approaches are illustrated through developments of test strips for detection of mycotoxins, veterinary drugs, and other analytes.The overall design of the immunochromatographic test strip is shown in Figure 1. It is a composite of several membranes of different structures and porosities, fixed on a support. The bundling of the test strip can vary, so it makes sense to consider its design based on what analytical tasks are being performed on its different sites.A. Typically, the lower portion of the test strip contains a sample pad. It ensures the absorption of sample components, in which the presence of the target analyte is checked.B. The following is a section with immunoreagents that are washed out during the analysis and move upward along with the components of the sample. As a rule, a conjugate pad forms this zone. It contains a conjugate of antibodies against the target analyte with a nanodispersed label-particles of colored latex, colloidal gold, and so on.The next two sections are located on the main working membrane of the test strip. C.First, there is a zone along which the movement of the absorbed components of the sample and the washed immunoreagents continues. During this movement, immune reactions occur, and specific intermolecular complexes are formed. D.Next, a mixture of reacted and unreacted molecules enters the binding area with immobilized immunoreagents. Depending on whether the target analyte was present in the sample and in what amount, binding of labeled immune complexes occurs in certain areas (in the traditional case, with the formation of narrow colored lines). Usually, additional reagents are located here to control the functionality of the test system. E. The upper part of the test strip with the final pad, usually structurally similar to the sample pad, ensures the further movement of the reaction mixture under the action of capillary forces and the washing of unreacted components from the underlying areas. These processes allow the label's binding to be evaluated correctly.Membrane components of the test strip are fixed on a plastic support and partia...

show abstract

Advantages of time-resolved fluorescent nanobeads compared with fluorescent submicrospheres, quantum dots, and colloidal gold as label in lateral flow assays for detection of ractopamine

Cited by 142 publications

References 32 publications

Comparative Study of Time-Resolved Fluorescent Nanobeads, Quantum Dot Nanobeads and Quantum Dots as Labels in Fluorescence Immunochromatography for Detection of Aflatoxin B1 in Grains

Comparative Study of Time-Resolved Fluorescent Nanobeads, Quantum Dot Nanobeads and Quantum Dots as Labels in Fluorescence Immunochromatography for Detection of Aflatoxin B1 in Grains

Dual signal insight: field-efficient qualitative/quantitative detection of sulphamethazine in raw milk

Ways to Reach Lower Detection Limits of Lateral Flow Immunoassays

Contact Info

Product

Resources

About