Adventitious Bud Formation from Bulb-scale Explants of Lilium speciosum Thunb. in vitro Effects of aminoethoxyvinyl-glycine, 1-aminocyclopropane-1-carboxylic acid, and ethylene

Aartrijk, J. van; Blom-Barnhoorn, G.J.; Bruinsma, J.

doi:10.1016/s0176-1617(85)80047-x

Cited by 29 publications

(8 citation statements)

References 26 publications

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“…Panizza et al (1988) observed that the shoot forming ability of stem node and flower spike explants of lavandin (Lavandula officinalis x L. latifolia) was linked to the degree of ethylene evolution from the cultures. Exogenous application of ethylene early in the culture period of Lilium speciosum increased the number of shoots which were formed on bulb-scale explants (Van Aartrijk et al 1985). Similarly, Kumar et al (1987) found that ethylene and carbon dioxide which built up during the first 10 to 15 days of culture promoted shoot bud formation in cotyledonary explants of Pinus radiata.…”

Section: Introductionmentioning

confidence: 89%

Promotion of petunia (Petunia hybrida L.) regeneration in vitro by ethylene

Dimasi-Theriou

Economou

Sfakiotakis

1993

Plant Cell Tiss Organ Cult

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The influence of ethylene on shoot and root formation from petunia leaf explants was studied in cultures in test tubes placed in 5 1 glass jars. Reduction of the endogenously produced ethylene by inclusion of ethysorb (KMnO4) , an ethylene absorbent, caused a decrease of the number of shoots. On the other hand, supplementing the cultures with ethylene (0.01-10 ppm) caused a marked increase of the number of shoots without, however, any effect on the length and fresh weight. Ethylene treatments (1 ppm) were found to be most effective when they were applied in the second week of culturing of petunia explants. Addition of Co ++ to the medium resulted in a reduction of the endogenously produced ethylene and concomitantly reduced shoot formation. Similarly, inclusion of Ag +, an inhibitor of ethylene action, resulted in poor shoot formation. Ethylene also appeared to play a role on rooting of petunia microshoots in vitro in an auxin-free medium. Ethylene at a concentration of 10 ppm induced adventitious root formation considerably, whereas at low levels (0.01-1ppm) it had no influence on rooting.

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Section: Introductionmentioning

confidence: 89%

Promotion of petunia (Petunia hybrida L.) regeneration in vitro by ethylene

Dimasi-Theriou

Economou

Sfakiotakis

1993

Plant Cell Tiss Organ Cult

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“…In bromeliads ethylene suppresses the apical dominance and thereby increases the number of axillary shoots that are formed [15] . Ethylene treatment increased the bud number per bulb-scale explant of Lilium speciosum in vitro [90] and enhanced the development of bulb primordia at the base of regenerated shoots of tulip [85] . Shoot-forming ability of lavandin is positively correlated with the rate of ethylene production in callus tissue and micropropagation explants [68] .…”

Section: 4 Effects Of Ethylene On Plant Growth and Development In Vmentioning

confidence: 99%

Components of the gaseous environment and their effects on plant growth and development in vitro

Buddendorf-Joosten

Woltering²

1994

Plant Growth Regul

107

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“…An increase in the ethylene production rate above a threshold is necessary for the outgrowth of lateral buds in Aechmea. Analogous observations were made on lateral bud outgrowth in Phaseolus vulgaris (19,20), Pisum sativum (1), and Lilium speciosum (14). A possible underlying control or involvement by cytokinins and auxins was not excluded in previous work.…”

Section: Discussionmentioning

confidence: 89%

Role of Ethylene and Cytokinins in the Initiation of Lateral Shoot Growth in Bromeliads

Dijck¹,

Proft²,

Greef³

1988

Plant Physiol.

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Aechmea victoriana var discolor L. B. Foster and Aechmea dactylina Bal. are commercially propagated in vitro through lateral shoot growth. A modified Murashige and Skoog medium is used which contains both BA and IAA. These growth substances were shown in the present study to synergistically stimulate the production of ethylene by the cultured plants. The stimulation of ethylene production is correlated with the outgrowth of the lateral buds. The rise in ethylene production was concluded to induce lateral shoot growth, because: (a) outgrowth of the shoots was blocked by preventing an increase in ethylene production, (b) 1-aminocyclopropane-1-carboxylic acid (ACC), the natural precursor of ethylene biosynthesis, substituted for IAA in the promotion of ethylene production and lateral bud outgrowth. Although ACC could substitute for IAA, it could not substitute for BA; therefore, cytokinins are concluded to be essential for lateral bud outgrowth in vitro in Aechmea. These results suggest that cytokinins and ethylene both play roles in natural lateral bud initiation and that the cytokinin function involves two stages of the process.The family Bromeliaceae is composed of tropical American genera, many of which are herbaceous epiphytes. Some of them are important ornamentals such as several Aechmea species (5). Several possibilities exist for the multiplication of bromeliads in vivo as well as in vitro (8). In vivo propagation of Aechmea through seed has been a standard practice. The plants obtained are variable and often of poor quality. Poor germination is often another problem which is associated with propagation through seed. Vegetative multiplication through suckers is too slow to be practical (22). Therefore, 'in vitro' propagation of Aechmea has become more and more widely used. To Ethylene Inhibitors. Suppression of the ethylene production was obtained by using: 5 mg/L AVG (10); 500 mg/L AIB (12, 13); 13 mg/L Co2+ (7), and for inhibition of ethylene action 66 mg/L STS (2, 17). RESULTSAfter in vitro germination, Aechmea plants were grown on a elongation medium containing only 1 mg/L 1-NAA during a first subculture of 10 weeks. These plants were used for experiments performed during subsequent subcultures. During the second subculture the pattern of the ethylene evolution differed with the type of culture medium used (Fig. 1)

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Adventitious Bud Formation from Bulb-scale Explants of Lilium speciosum Thunb. in vitro Effects of aminoethoxyvinyl-glycine, 1-aminocyclopropane-1-carboxylic acid, and ethylene

Cited by 29 publications

References 26 publications

Promotion of petunia (Petunia hybrida L.) regeneration in vitro by ethylene

Promotion of petunia (Petunia hybrida L.) regeneration in vitro by ethylene

Components of the gaseous environment and their effects on plant growth and development in vitro

Role of Ethylene and Cytokinins in the Initiation of Lateral Shoot Growth in Bromeliads

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