Adventitious bud induction in Eucalyptus globulus Labill

Nugent, Greg; Chandler, Stephen F.; Whiteman, Phil H.; Stevenson, Trevor W.

doi:10.1007/s11627-001-0068-0

Cited by 25 publications

(9 citation statements)

References 14 publications

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“…Na micropropagação de Eucalyptus, o meio de cultura MS (MURASHIGE e SKOOG, 1962) tem sido o mais utilizado (BENNETT et al, 1994;YANG et al, 1995;SHARMA e RAMAMURTHY, 2000;GRAÇA et al, 2001;NUGENT et al, 2001;SOBROSA e CORDER, 2003;WATT et al, 2003;BILLARD et al, 2005;GLOCKE, et al, 2006;BRONDANI et al, 2009). O meio JADS (CORREIA, 1993) também tem sido testado com sucesso em alguns trabalhos de micropropagação com as espécies do gênero (LIMA e GONÇALVES, 1998;SANTOS et al, 2004;ANDRADE et al, 2006;QUISEN, 2007;BRAVO et al, 2008).…”

Section: Introductionunclassified

Multiplicação in vitro de clones híbridos de Eucalyptus globulus

et al. 2011

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Multiplicação in vitro de clones híbridos de... MULTIPLICAÇÃO IN VITRO DE CLONES HÍBRIDOS DE Eucalyptus globulus 1RESUMO -O presente estudo teve como objetivos avaliar a resposta de clones híbridos de Eucalyptus globulus nos meios de cultura MS e JADS durante a fase de multiplicação in vitro. Os explantes foram provenientes da fase de estabelecimento in vitro de 21 clones de Eucalyptus urophylla x E. globulus, sendo 11 clones com três introduções in vitro, e de seis clones de Eucalyptus grandis x E. globulus, sendo dois clones com três introduções. Os clones foram subcultivados mensalmente nos meios de cultura MS e JADS, com 0,5 mg L -1 de BAP (6-benzilaminopurina) e 0,01 mg L -1 de ANA (ácido naftalenoacético). Concluiu-se que: houve tendência de maiores taxas de multiplicação no meio MS; a maioria dos clones se estabeleceu na fase de multiplicação in vitro, enquanto alguns clones se mostraram recalcitrantes nesta fase da micropropagação; a taxa de multiplicação apresentou tendência de aumento nos primeiros subcultivos e posterior queda para a maioria dos clones. IN VITRO MULTIPLICATION OF HYBRID CLONES OF Eucalyptus globulus ABSTRACT -The present study aimed to evaluate the response of hybrid Eucalyptus globulus clones in MS and JADS culture media during the in vitro multiplication phase. The explants were originated from the in vitro establishment phase of 21 Eucalyptus urophylla x E. globulus clones, being 11 clones with three in vitro introductions, and of six Eucalyptus grandis x E. globulus clones, being two clones with three introductions.The clones were subcultivated monthly in MS and JADS culture media,

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Section: Introductionunclassified

Multiplicação in vitro de clones híbridos de Eucalyptus globulus

et al. 2011

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“…However, in order to undertake an effective genetic improvement programme, an efficient regeneration protocol (through shoot organogenesis and/or somatic embryogenesis) is required (Le Roux and Van Staden 1991). In vitro propagation through shoot organogenesis (Barrueto Cid et al 1999;Dibax et al 2005;Nugent et al 2001a;Mullins et al 1997;Muralidharan and Mascarenhas 1987) and somatic embryogenesis (Nugent et al 2001b;Pinto et al 2008) has been reported in a number of Eucalyptus species. However, there have been very few studies on shoot organogenesis (Subbaiah and Minocha 1990) and somatic embryogenesis (Prakash and Gurumurthi 2005) in E. tereticornis.…”

Section: Introductionmentioning

confidence: 99%

Shoot organogenesis in elite clones of Eucalyptus tereticornis

Aggarwal

Kumar

Reddy

2010

Plant Cell Tiss Organ Cult

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An efficient shoot organogenesis system has been developed from mature plants of selected elite clones of Eucalyptus tereticornis Sm. Cultures were established using nodal explants taken from freshly coppice shoots cultured on Murashige and Skoog medium containing 58 mM sucrose, 0.7% (w/v) agar (MS medium) and supplemented with 2.5 lM benzyladenine (BA) and 0.5 lM a-naphthaleneacetic acid (NAA). Shoot organogenesis was achieved from leaf segments taken from elongated microshoots on MS medium supplemented with 5.0 lM BA and 1.0 lM 2,4-dichlorophenoxyacetic acid (2,4-D). The addition of cefotaxime to the medium promoted shoot differentiation, whereas carbenicillin and cephalexin inhibited shoot differentiation. Maximum shoot bud organogenesis (44.6%) occurred in explants cultured on MS medium supplemented with 5.0 lM BA, 1.0 lM 2,4-D and 500 mg/l cefotaxime. Leaf maturity influenced shoot regeneration, with maximum shoot organogeneisis (40.5%) occurring when the source of explants was the fifth leaf (14-16 days old) from the top of microshoot. Shoot organogenic potential also varied amongst the different clones of E. tereticornis. Random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) analyses indicated clonal uniformity of the newly formed shoots/ plants, and these were also found to be true-to-type.

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“…Thidiazuron (TDZ) and 2-Cl-PBU (i.e., 1-phenyl-3-(1,2,3-thiadiazol-5-yl) urea and N-phenyl-N -[6-(2-chlorobenzothiazol)-yl] urea, respectively) are potent promotors of callus formation in eucalypts. Organogenesis can be induced using 0.89 µM BA with 0.91 µM TDZ, or 0.5 µM TDZ with 0.2 µM 2,4-D for E. globulus [127,128], 2 µM TDZ, or 0.23 µM TDZ with 0.05 µM NAA, or 3 µM TDZ with 0.1 µM NAA for E. grandis × E. urophylla [89,123,128], and 2.27 µM TDZ with 0.54 µM NAA, or 1.14 µM 2-Cl-PBU with 0.57 µM IAA for E. urophylla [89,111,112]. Picloram (i.e., 4-Amino-3,5,6-trichloro-2-pyridinecarboxylic acid) has been used alone at 20.7 µM for E. urophylla organogenesis [86] or at 0.04 µM in combination with 2.25 µM BA for E. gunnii Hook.f.…”

Section: Organogenesismentioning

confidence: 99%

Tissue Culture of Corymbia and Eucalyptus

Trueman

Hung

Wendling

2018

Forests

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Eucalypts are among the world's most widely planted trees, but the productivity of eucalypt plantations is limited by their often-low amenability to true-to-type propagation from cuttings. An alternative approach to cutting propagation is tissue culture, which can be used to micropropagate valuable genotypes rapidly while simultaneously preserving germplasm in vitro. This review describes the use of tissue culture methods such as shoot culture, organogenesis, and somatic embryogenesis for micropropagating eucalypts. This review also discusses the use of cool storage, encapsulation, and cryopreservation methods for preserving eucalypt germplasm and delaying tissue maturation under minimal-growth conditions.

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Adventitious bud induction in Eucalyptus globulus Labill

Cited by 25 publications

References 14 publications

Multiplicação in vitro de clones híbridos de Eucalyptus globulus

Multiplicação in vitro de clones híbridos de Eucalyptus globulus

Shoot organogenesis in elite clones of Eucalyptus tereticornis

Tissue Culture of Corymbia and Eucalyptus

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