Adventitious rooting in apple rootstock MM.106: Effects of arbuscular mycorrhizal fungi (AMF) and plant growth regulators

Krishna, Hare; Attri, Brij Lal; Kumar, Akhilesh; Ahmed, Nazeer; Maheshwari, Sushil. K.; Joshi, Harish C.; Lal, Niranjan

doi:10.1080/14620316.2013.11512969

Cited by 4 publications

(3 citation statements)

References 22 publications

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“…In the present study, large and green leaves of inoculated seedlings could have a highly effective photosynthesis rate, which is beneficial for the accumulation of carbohydrates. In addition, AMFs inoculation enhances the root number by phytohormone regulating [32] and then influences root architecture by endogenous polyamines metabolism [33]. Moreover, AMFs inoculation equips extensive extra-radical mycelium, which would increase surface areas of roots in order to acquire more nutrients and water [34].…”

Section: Growth Of S Miltiorrhiza With Amfs Inoculationmentioning

confidence: 99%

Arbuscular Mycorrhizal Fungi Regulate the Growth and Phyto-Active Compound of Salvia miltiorrhiza Seedlings

Yang

Guang

et al. 2017

Applied Sciences

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Roots and rhizomes of Salvia miltiorrhiza (S. miltiorrhiza) are widely used for the treatment of cardiovascular diseases. Arbuscular mycorrhizal fungi (AMFs) have been shown to enhance plant growth and increase secondary metabolites concentration in many plant species. However, effects of AMFs on S. miltiorrhiza have not been explored. A pot culture was designed as one control (non-AMF) treatment and four AMFs (G.m, Glomus mosseae; G.a, Glomus aggregatum; G.v, Glomus versiforme; G.i, Glomus intraradices) treatments were performed in order to evaluate the effects of AMFs on plant growth, as well as phyto-active compounds' concentration of S. miltiorrhiza seedlings. Plants were harvested after 90 days: agronomic traits and concentration; and an accumulation of mineral elements, as well as phyto-active compounds were detected. All AMFs inoculated plants formed mycorrhizal structures, and an infection ratio; also, the intensity of inoculated roots was higher than 84.61% and 23.86%, respectively. Mycorrhizal dependency was above 144.62%. Seedlings with AMFs inoculation had significantly higher plant height, leather leaf length, top leaflet size, base leaflet length, taproot length, taproot diameter and biomass than those with non-AMF inoculation. In addition, inoculation with AMFs increased N, P, and K accumulation significantly, but barely had any effect on mineral elements' concentrations. AMFs inoculation also significantly improved tanshinones concentrations and stimulation in order to accumulate salvianolic acid B. G.v and G.i were effective for seedlings growth; G.m and G.i were also effective for phyto-active compounds. In total, S. miltiorrhiza inoculation with AMFs had positive effects on growth and active components, especially inoculation with G.v.

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Section: Growth Of S Miltiorrhiza With Amfs Inoculationmentioning

confidence: 99%

Arbuscular Mycorrhizal Fungi Regulate the Growth and Phyto-Active Compound of Salvia miltiorrhiza Seedlings

Yang

Guang

et al. 2017

Applied Sciences

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“…Adventitious root (AR) formation is the most critical step in cutting propagation, and a good root system architecture is crucial to the survival rate and resistance of seedlings to various stresses after transplanting ( Chen et al., 2016 ). Previous studies have shown that arbuscular mycorrhizal fungus (AMF), which can form a symbiotic relationship with the roots of most terrestrial plants, has great potential to affect plant cutting propagation ( Ávila Díaz-Granados et al., 2009 ; Krishna et al., 2013 ; Essahibi et al., 2017 ; Fernades et al., 2019 ) and construction of root system architecture ( Chen et al., 2016 ), however, there are still few studies on the effect of AMF on the rooting of tea cuttings ( Liu and Lei, 1995 ; Gao et al., 2023 ), which mainly focused on the growth of tea seedlings in the later stage without covering the regulatory mechanism of adventitious root formation induced by AMF in the early stage. Therefore, in this paper, in order to decipher the relative mechanism, we divided the rooting state of tea cuttings into four stages for the first time ( Figure 1 ) : S0 (stage of non-rooting), S1 [stage of adventitious root (AR) protrusion], S2 (stage of AR formation) and S3 (stage of AR elongation).…”

Section: Discussionmentioning

confidence: 99%

Insight into regulation of adventitious root formation by arbuscular mycorrhizal fungus and exogenous auxin in tea plant (Camellia sinensis L.) cuttings

Chen,

Shan,

Niu

et al. 2023

Front. Plant Sci.

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IntroductionAdventitious root (AR) development, affected by various biotic and abiotic factors, is the most important procedure in tea plant (Camellia sinensis L.) cutting propagation. Establishing symbiotic relationships with most terrestrial plants, AMF (Arbuscular mycorrhizal fungus) can mediate the AR formation of several herbaceous and woody plants in previous studies.MethodsIn this paper, effects of combined application of AMF and exogenous auxin on AR formation of cuttings from different tea plant varieties (‘Pingyangtezao’, ‘Longjing 43’ and ‘Longjingchangye’) were studied. Then we also performed RNA-Seq analysis with ‘Pingyangtezao’ cuttings aiming to find the possible auxin-related pathway of AM fungal regulation on AR formation. To accurately uncover the regulatory mechanism of AMF on AR formation of tea cuttings, rooting process were separated into four stages (S0, non-rooting; S1, AR protrusion; S2, AR formation and S3, AR elongation) at the same sampling time.Results and DiscussionResults showed that IBA treatment increased the mycorrhizal colonization rate, especially in ‘Pingyangtezao’ variety (from 37.58% to 46.29%). Both inoculating AMF and addition of IBA promoted the AR formation, and rooting of different tea plant varieties showed different dependence on auxin. AMF could alleviate the effect of auxin-related inhibitors (2,3,5-triiodobenzoic acid, L-α-(Aminooxy)-β-phenylpropionic acid and α-(phenylethyl-2-oxo)-IAA) on rooting of tea cuttings, even though the colonization of AMF was hindered at various degrees. Transcriptomic analysis showed that different numbers of differentially expressed genes (DEGs) at various rooting stages of tea cuttings with the most at S2 stage (1360 DEGs), indicating the increasing regulation by AMF with the development of AR. Similar trend was found in auxin-related DEGs, and family genes of YUC, GH, PIN, LAX, SAUR, AUX, and ABP involved in the AM fungal regulation on AR formation of tea cuttings. Additionally, AMF strongly mediated auxin transport and signal transduction pathways in tea cuttings as showed by the results of correlation analysis. Overall, interaction of AMF and exogenous auxin in promoting rooting and the preliminary mechanism of AMF regulating AR formation of tea cuttings was deciphered in this paper, which may provide a basis for further deep mechanistic research and cutting propagation of tea production.

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“…Rooting is a unique way of organogenesis in plant (Valdes et al 2001 ). The ability of rooting depends on the plant species and the culture condition (Devi et al 2013 ; Krishna et al 2013 ; Mendes et al 2011 ). Understanding how somatic cells differentiate into organ is very important for us to investigate plant development (Couee et al 2004 ; Mendes et al 2011 ; Valdes et al 2001 ).…”

Section: Introductionmentioning

confidence: 99%

MicroRNAs, polyamines, and the activities antioxidant enzymes are associated with in vitro rooting in white pine (Pinus strobus L.)

et al. 2016

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Molecular mechanism of in vitro rooting in conifer is not fully understood. After establishment of a regeneration procedure in eastern white pine (Pinus strobus L.) using mature embryos as explants to induce shoot formation on medium containing 3 μM IAA, 6 μM BA and 6 μM TDZ and induce root formation on medium containing 0.001-0.05 μM IAA, 0.001–0.05 μM IBA, 0.001–0.05 μM TDZ, we have investigated the changes of polyamine content and the activities of antioxidant enzymes during in vitro rooting in P. strobus. Our results demonstrated that putrescine (Put), spermidine (Spd), and spermine (Spm) did not increase in P. strobus during the first week of rooting on medium supplemented with 0.01 μM indole-3-acetic acid (IAA), whereas the levels of Put, Spd, and Spm increased during the 1st–3rd week of culture on medium with IAA, and then decreased on medium with IAA. No such a change in Put, Spd, and Spm was observed on medium without IAA. Measurement of antioxidant enzyme activity demonstrated that the activities of polyphenol oxidase, catalase, and peroxidase slightly increased in the first week of culture and reached to the highest peak in the 3rd–5th week of culture. Quantitative RT-PCR results indicated that miR160 was increased on the 7th day, miR162, miR397, and miR408 was increased from the 21th to 35th day, miR857 was increased on the 35th day, and miR827 was increased on the 49th day. These results demonstrated that enhanced polyamine biosynthesis, antioxidant enzyme activity, and microRNAs are correlated with the root induction and formation in P. strobus.

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Adventitious rooting in apple rootstock MM.106: Effects of arbuscular mycorrhizal fungi (AMF) and plant growth regulators

Cited by 4 publications

References 22 publications

Arbuscular Mycorrhizal Fungi Regulate the Growth and Phyto-Active Compound of Salvia miltiorrhiza Seedlings

Arbuscular Mycorrhizal Fungi Regulate the Growth and Phyto-Active Compound of Salvia miltiorrhiza Seedlings

Insight into regulation of adventitious root formation by arbuscular mycorrhizal fungus and exogenous auxin in tea plant (Camellia sinensis L.) cuttings

MicroRNAs, polyamines, and the activities antioxidant enzymes are associated with in vitro rooting in white pine (Pinus strobus L.)

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