Adventitious shoot regeneration and <i>Agrobacterium tumefaciens</i>-mediated transformation of leaf explants of sweet cherry (<i>Prunus avium</i> L.)

Zong, Xiaojuan; Chen, Qiuxia; Nagaty, Mohamed A.; Kang, Yunyan; Lang, Gregory A.; Song, Guo Qing

doi:10.1080/14620316.2018.1470908

Cited by 7 publications

(4 citation statements)

References 28 publications

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“…Successful in vitro shoot regeneration from seed-derived or leaf explants has been reported for several Prunus species, such as P. persica (Druart, 1999;Gentile et al, 2002;Hammerschlag et al, 1985;Mante et al, 1989;Pooler and Scorza, 1995;Scorza et al, 1990), P. canescens (Antonelli and Druart, 1989), P. padus (Hammatt, 1993), P. domestica Cossio, 1991, 1994;Yancheva, 1993), P. dulcis (Ainsley et al, 2000;Miguel and Oliveira, 1999;Miguel et al, 1996), P. armeniaca (P erez-Tornero et al, 2000), P. serotina (Espinosa et al, 2006;Hammatt and Grant, 1998), P. avium (Bhagwat and Lane, 2004;Canli and Tian, 2008;Feeney et al, 2007;Hammatt and Grant, 1998;Matt and Jehle, 2005;Tang et al, 2002;Zong et al, 2019), P. cerasus (Mante et al, 1989;Sarropoulou et al, 2012;Song and Sink, 2006;Tang et al, 2002), and several hybrid rootstocks (Hasan et al, 2010;Ochatt et al, 1988;P erez-Jim enez et al, 2012P erez-Jim enez et al, , 2014Pooler and Scorza, 1995;San et al, 2015;Sarropoulou et al, 2012;Zhou et al, 2010). For most cultivars of Prunus species, leaf explants are preferable to seedderived explants for maintaining an identical genetic background of the cultivars because of their heterozygosity ...…”

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confidence: 99%

Adventitious Shoot Regeneration and Agrobacterium tumefaciens-mediated Transient Transformation of Almond × Peach Hybrid Rootstock ‘Hansen 536’

Zong¹,

Denler²,

Danial³

et al. 2019

horts

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Hansen 536' (Prunus dulcis 3 Prunus persica) is an important commercial rootstock for peach and almond. However, susceptibility to wet soil and bacterial canker has limited its use primarily to areas with less annual rainfall. Genetic engineering techniques offer an attractive approach to improve effectively the current problems with this cultivar. To develop an efficient shoot regeneration system from leaf explants, 10 culture media containing Murashige and Skoog (MS) or woody plant medium (WPM) supplemented with different plant growth regulators were evaluated, and adventitious shoot regeneration occurred at frequencies ranging from 0% to 36.1%. Optimal regeneration with a frequency of 32.3% to 36.1% occurred with WPM medium containing 8.88 mM 6-benzylamino-purine (BAP) and 0.98 to 3.94 mM indole-3-butyric acid (IBA). The regenerated shoots had a high rooting ability, and 80% of the in vitro shoots tested rooted and survived after being transplanted to substrate directly. Transient transformation showed an efficient delivery of the b-glucuronidase (GUS) reporter gene (gusA) using all three Agrobacterium tumefaciens strains tested with a concentration of OD 600 0.5 to 1.0 for 4 days of cocultivation. The protocols described provide a foundation for further studies to improve shoot regeneration and stable transformation of the important peach and almond rootstock 'Hansen 536'.

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confidence: 99%

Adventitious Shoot Regeneration and Agrobacterium tumefaciens-mediated Transient Transformation of Almond × Peach Hybrid Rootstock ‘Hansen 536’

Zong¹,

Denler²,

Danial³

et al. 2019

horts

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“…In general, plants with high regeneration capability from non-meristem containing explants are amenable for genetic transformation. Unlike annual crops (e.g., wheat, maize, rice, and soybean), the cultivated F&N crops, for example, blueberries (Song and Hancock, 2012) and cherries (Song, 2014; Zong et al, 2018), are often clonally propagated; to maintain genetic stability, regeneration from explants of clonally propagated tissues is preferable. Lack of available explants sources (e.g., seedlings) makes plant regeneration studies for F&N crops a lengthy process (Figure 3), because in many instances an efficient micropropagation system has to be established for obtaining regenerable explants (e.g., leaves and petioles) prior to regeneration studies (Kole and Hall, 2008).…”

Section: Key Limitations For Transformation Of Fandn Cropsmentioning

confidence: 99%

“…In addition, little has been documented on the potential impacts of plant- Agrobacterium interaction on plant regeneration. Finally, for some extremely recalcitrant species in the group (e.g., Prunus persica ) species, regeneration can be achieved mostly on the basis of the use of seed explants (Song, 2014; Petri et al, 2018; Zong et al, 2018). In terms of using transgenes to improve regeneration efficiency, one report demonstrated that constitutive expression of the class I KNOX gene of maize increased production of adventitious shoots from leaf explants of plum (Srinivasan et al, 2011).…”

Section: Key Limitations For Transformation Of Fandn Cropsmentioning

confidence: 99%

Agrobacterium-Mediated Transformation of Tree Fruit Crops: Methods, Progress, and Challenges

2019

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Genetic engineering based on Agrobacterium -mediated transformation has been a desirable tool to manipulate single or multiple genes of existing genotypes of woody fruit crops, for which conventional breeding is a difficult and lengthy process due to heterozygosity, sexual incompatibility, juvenility, or a lack of natural sources. To date, successful transformation has been reported for many fruit crops. We review the major progress in genetic transformation of these fruit crops made in the past 5 years, emphasizing reproducible transformation protocols as well as the strategies that have been tested in fruit crops. While direct transformation of scion cultivars was mostly used for fruit quality improvement, biotic and abiotic tolerance, and functional gene analysis, transgrafting on genetically modified (GM) rootstocks showed a potential to produce non-GM fruit products. More recently, genome editing technology has demonstrated a potential for gene(s) manipulation of several fruit crops. However, substantial efforts are still needed to produce plants from gene-edited cells, for which tremendous challenge remains in the context of either cell’s recalcitrance to regeneration or inefficient gene-editing due to their polyploidy. We propose that effective transient transformation and efficient regeneration are the key for future utilization of genome editing technologies for improvement of fruit crops.

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“…Development of a reliable regeneration system for somatic tissue is crucial for the improvement of woody fruit species by transgenic approaches 7 . Callus induction is the first step in the development of a transformation protocol as callus is usually used for genetic transformation and adventitious shoot regeneration [8][9][10] . A variety of explants, such as leaf, stem and calyx, have been successfully used to induce callus production in peach 11 .…”

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Development of a fast and efficient root transgenic system for functional genomics and genetic engineering in peach

Lai

Zhao

et al. 2020

Sci Rep

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Peach is an economically import fruit crop worldwide, and serves as a model species of the Rosaceae family as well. However, peach functional genomics studies are severely hampered due to its recalcitrance to regeneration and stable transformation. Here, we report a fast and efficient Agrobacterium rhizogenes-mediated transformation system in peach. Various explants, including leaf, hypocotyl and shoot, were all able to induce transgenic hairy roots, with a transformation efficiency of over 50% for hypocotyl. Composite plants were generated by infecting shoots with A. rhizogenes to induce transgenic adventitious hairy roots. The composite plant system was successfully used to validate function of an anthocyanin-related regulatory gene PpMYB10.1 in transgenic hairy roots, and two downstream genes, PpUFGT and PpGST, were strongly activated. Our stable and reproductive A. rhizogenes-mediated transformation system provides an avenue for gene function assay, genetic engineering, and investigation of root-rhizosphere microorganism interaction in peach. open Scientific RepoRtS | (2020) 10:2836 | https://doi.

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Adventitious shoot regeneration and Agrobacterium tumefaciens-mediated transformation of leaf explants of sweet cherry (Prunus avium L.)

Cited by 7 publications

References 28 publications

Adventitious Shoot Regeneration and Agrobacterium tumefaciens-mediated Transient Transformation of Almond × Peach Hybrid Rootstock ‘Hansen 536’

Adventitious Shoot Regeneration and Agrobacterium tumefaciens-mediated Transient Transformation of Almond × Peach Hybrid Rootstock ‘Hansen 536’

Agrobacterium-Mediated Transformation of Tree Fruit Crops: Methods, Progress, and Challenges

Development of a fast and efficient root transgenic system for functional genomics and genetic engineering in peach

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