Adventitious shoot regeneration from juvenile cotyledons of a biodiesel producing Plant, Jatropha curcas L

Khemkladngoen, Naruemon; Cartagena, Joyce A.; Shibagaki, Nakako; Fukui, Kiichi

doi:10.1016/j.jbiosc.2010.09.002

Cited by 39 publications

(27 citation statements)

References 14 publications

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“…Seeds of the Philippine line were provided by University of the Philippines Los Baños, and those of Thai and Tanzanian lines were provided by Arid Land Research Center, Tottori University. The cotyledons were prepared as described in Khemkladngoen et al (2011a). Alternatively, leaves were sterilized for 10 min in 10% Sodium hypochlorite and 0.1% Tween 20, and rinsed 3 times with sterile distilled water.…”

Section: Plant Materials and Preparation Of Explantsmentioning

confidence: 99%

“…erefore, the shoot regeneration medium for leaf explants was slightly modi ed. Since thidiazuron (TDZ) is useful for inducing a large number of shoot buds or shoots in jatropha (Deore and Johnson 2008;Khemkladngoen et al 2011a), TDZ was added to the shoot regeneration medium (SR-I) to induce shoot buds in the rst two weeks of culture. To eliminate the carry-over e ect of TDZ, the leaf explants were transferred to the shoot regeneration medium without TDZ (SR-II) for further shoot multiplication and regeneration.…”

Section: Improvement Of the Transformation Protocol For Generating Trmentioning

confidence: 99%

“…In the process of establishing a transformation method for jatropha, the regeneration of shoots from tissue explants also proved to be challenging. Despite the fact that there are several existing reports on shoot regeneration from different tissues of jatropha including our own work (Khemkladngoen et al 2011a), regeneration conditions such as hormone concentrations and the timing of subjecting explants to certain growth hormones should be considered carefully. e step of root induction is also challenging because the resultant rate is usually low.…”

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confidence: 99%

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Development of transgenic plants in jatropha with drought tolerance

Tsuchimoto

Cartagena

Khemkladngoen

et al. 2012

Plant Biotechnology

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Abstracte seed oil of jatropha (Jatropha curcas L.) is a source of biodiesel fuel. Although jatropha can grow in semiarid lands unsuitable for the food production, its oil productivity in such conditions is unsatisfactory at present. erefore, it is desirable to improve the oil productivity of jatropha even in semi-arid lands by enhancing its drought tolerance. Genetic engineering is promising to dramatically improve plant traits. Although we previously reported a transformation method, which involves wounding of tissue explants in order to increase the chance of Agrobacterium infection, for jatropha, it remains a challenge to enhance the shoot regeneration and root induction processes. Here, we report the generation of three kinds of transgenic jatropha plants in an attempt to improve their drought tolerance. e rst one overexpresses the PPAT gene, which encodes an enzyme that catalyzes the CoA biosynthetic pathway; the second overexpresses the NF-YB gene, which encodes a subunit of the NF-Y transcription factor; and the last overexpresses the GSMT and DMT genes, which encode enzymes that catalyze production of glycine betaine. We also report a modi ed protocol that improves the e ciency of shoot regeneration and root induction in transgenic jatropha plantlets.

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Section: Plant Materials and Preparation Of Explantsmentioning

confidence: 99%

Section: Improvement Of the Transformation Protocol For Generating Trmentioning

confidence: 99%

mentioning

confidence: 99%

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Development of transgenic plants in jatropha with drought tolerance

Tsuchimoto

Cartagena

Khemkladngoen

et al. 2012

Plant Biotechnology

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“…Furthermore, the pre-condition of carrying out genetic transformation successfully is that adventitious buds can be feasible regenerated from the transformed explants (receptors) via tissue culture. Nevertheless, it's need to spend 3-5 months in obtaining complete plantlets generally by applying the conventional regeneration methods in J. curcas [10][11][12][13]. Going one step further, the regeneration efficiency is still not satisfactory [10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

“…Nevertheless, it's need to spend 3-5 months in obtaining complete plantlets generally by applying the conventional regeneration methods in J. curcas [10][11][12][13]. Going one step further, the regeneration efficiency is still not satisfactory [10][11][12][13]. As a consequence, in order that the improved varieties can be cultivated via the genetic improvement method, it will be necessary to establish an efficient regeneration protocol properly to enhance the efficiency of adventitious buds regeneration and shorten the circle of tissue culture.…”

Section: Introductionmentioning

confidence: 99%

High-frequency Adventitious Buds Induction from Leaf Explants of Jatropha Curcas L.

Liu¹,

Lu²,

Niu³

et al. 2017

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Towards the Domestication of Jatropha: The Integration of Sciences

Carels

2012

Jatropha, Challenges for a New Energy Crop

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N. Carels approximately 170 known species. Recent phylogenetic studies in the genus Jatropha based on random ampli fi ed polymorphic DNA (RAPD), ampli fi ed fragment length polymorphisms (AFLP, Vos et al. 1995 ) and nuclear ribosomal-DNA internal transcribed spacer (nrDNA ITS) markers (Sudheer et al. 2009a ) showed that J. curcas is genetically more similar to J. integerrima Jacq. than to any other species of genus Jatropha .J. curcas is a stem-succulent deciduous tree of ~5 m in height that produces seeds rich in oil (27-40%). Crude oil of J. curcas meets the fuel quality standards of rapeseed and can be easily converted into biodiesel that meets US and European standards by transesteri fi cation.J. curcas is currently considered as undomesticated; its physiology and agronomy remain to be clari fi ed, and thus seed yield is poorly predictable (Ginwal et al. 2004 ) . However, in the interval of just 3 years from 2007 to 2010, great efforts have been made by the scienti fi c community, particularly in India, to gather data on the genetic diversity and interspeci fi c hybridization of this species, as well as from analyses using the complete range of available biotechnologies, including in vitro propagation, tissue culture, genetic transformation, transcriptome description and genome sequencing. Forward and reverse genomics approaches are now beginning to be employed in J. curcas research.The twenty-fi rst century is witnessing an unprecedented level of scienti fi c integration, which in the case of biology, has led to the concept of systems biology . This concept can be de fi ned as the study of the interactions between the components of biological systems and how these interactions give rise to the functioning and behavior of that system. In the case of genetics, the system also includes the environment of the biological entity under consideration because it conditions the spectrum of stimuli that are used to score the genome performance. As a consequence, systems biology implies high-throughput technologies of data collection, information technology for data management and mathematical modeling.Genetic correlations can initially be investigated by examining domesticated crops as an entity that represents a particular mode of the expression of a plant species. Related to this notion, there is a common suite of traits known as the "domestication syndrome" (Doebley et al. 2006 ;Tang et al. 2010 ) , which represents the kind of complexity that is appropriate to the new perspective that plant breeding must be addressed with the support of systems biology.Here, I propose to discuss the current knowledge and emerging technologies available from model crops with the underlying prospect of their application to the selective breeding of J. curcas . Mating SystemJ. curcas is monoecious, with separate male and female fl owers, and is a predominantly outbreeding species, as shown by its high abortion rate, low seed-ovule ratio, low fecundity rate, high pollinator attractiveness, delayed stigma receptive

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Adventitious shoot regeneration from juvenile cotyledons of a biodiesel producing Plant, Jatropha curcas L

Cited by 39 publications

References 14 publications

Development of transgenic plants in jatropha with drought tolerance

Development of transgenic plants in jatropha with drought tolerance

High-frequency Adventitious Buds Induction from Leaf Explants of Jatropha Curcas L.

Towards the Domestication of Jatropha: The Integration of Sciences

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