Adventitious shoot regeneration of Platanus acerifolia Willd. facilitated by Timentin, an antibiotic for suppression of Agrobacterium tumefaciens in genetic transformation

Abstract

The effects of Timentin and cefotaxime (Cef) on shoot regeneration of the London plane tree (Platanus acerifolia Willd.) and their use for the suppression of Agrobacterium tumefaciens in Agrobacterium-mediated genetic transformation were compared. Shoot regeneration was significantly reduced on the media with Cef at concentrations from 100 to 500 mg·L -1 . Timentin showed negative effect on plant regeneration at concentrations of 100 and 500 mg·L -1 ; however, 300 mg·L -1 Timentin was shown to facilitate shoot regeneration significantly and the regeneration frequency increased from 64% (control) to 88%. Effective suppression of A. tumefaciens could be obtained with 500 mg·L -1 Cef, but plant regeneration was completely inhibited at this level. The A. tumefaciens on infected P. acerifolia leaf tissues was visually undetectable after three subcultures on a medium with 300 mg·L -1 Timentin. Considering the effect of Cef and Timentin on plant regeneration and suppression of Agrobacteria, Timentin at 300 mg·L -1 is the preferred application in A. tumefaciens-mediated transformation of P. acerifolia.

Genetic transformation of gentian Gentiana tibetica (King) leaf explants with Agrobacterium tumefaciens strain C58C1

“…There are numerous evidences giving examples of non-destructive effect of b-lactam antibiotics on eukaryotic organisms (Pollock et al 1983;Young et al 1984;d'Utra Vaz et al 1993;Kaur et al 2008). However, we should note information on the inhibition action or delayed response of plant explants cultured in the presence of cefatoxime (Cheng et al 1998;Joersbo et al 2003;Li et al 2007). A similar phenomenon was observed in the case of our experiments.…”

“…It has a significant meaning in the case of species in which cefatoxime negatively affects the regeneration processes (Cheng et al 1998). The timentin may not only stimulate inhibition of the morphogenic response of explant but enhance it as well, which was observed in the case of leaf explants of Dianthus caryophyllus, Lycopersicon esculentum, or Platanus acerifolia (Estopà et al 2001;Li et al 2007;Mamidala and Nanna 2009a, b). We would like to stress that such test of the effect of b-lactam antibiotics on morphogenetic potential has never been done before for gentians and more so their leaf explants.…”

Ticarcillin degradation product thiophene acetic acid is a novel auxin analog that promotes organogenesis in tomato

“…Previous studies have reported a positive effect of β-lactam ring-containing antibiotics such as timentin, carbenicillin, and penicillin G on shoot regeneration in species such as tomato, tobacco, and London plane tree. This effect was found to be dependent on the antibiotic concentration and plant genotype ( Nauerby et al., 1997 ; Ling et al., 1998 ; Costa et al., 2000 ; Li et al., 2007 ; Varlamova et al., 2021 ). In contrast, some studies have also reported no significant effect of these antibiotics on shoot regeneration in species such as tobacco and Siberian elm ( Cheng et al., 1998 ).…”

Effect of Ceftriaxone and Timentin antibiotics on morphogensis in the in vitro culture of bread wheat Triticum aestivum L.

“…It is established that the influence of Timentin on regeneration processes depends on its concentration in culture medium [14,15]: during shoots regeneration of London plane tree (Platanus acerifolia Willd) from leaf explants, Timentin in concentration of 100 and 500 mg/l adversely affects plant regeneration, and in concentration of 300 mg/l -significantly increased shoot regeneration frequency [14]; during hypocotyls and cotyledons regeneration of teak (Tectona grandis) Timentin in concentration of 100-300 mg/l stimulated shoots regeneration, and in concentration 500 mg/l induced excessive calli formation, but inhibited regeneration [15]. These data confirm our observation that Timentin in high concentrations inhibits regenerative processes, and in average -can stimulate them.…”

“…Plant sensitivity to antibiotics is not only speciesspecific, but also genotype-dependent, therefore in our study we used two wheat genotypes of different growth habit, winter wheat and winter-spring wheat. Although the β-lactam antibiotics are considered to be non-toxic to plants [12], many researchers have noted that their effect on the regeneration and morphogenesis may vary significantly depending on the plant species and the antibiotic concentration [14,15]. Hence, in order to establish the pattern of action of the new generation antibiotics of β-lactam group on the morphogenetic processes (morphogenic callus development, shoot regeneration and rhizogenesis) in wheat, we used two antibiotics -Timentin and Ceftriaxone.…”