Adverse effects of 2-Methoxyestradiol on mouse oocytes during reproductive aging

Jiang, Xiuying; Xu, Xiangning; Wang, Bi-Cheng; Song, Ke; Zhang, Jiaqi; Chen, Ye; Tian, Ying; Weng, Jing Ru; Liang, Yuanzheng; Ma, Wei

doi:10.1016/j.cbi.2022.110277

Cited by 5 publications

(3 citation statements)

References 44 publications

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“…This is in line with previous research, which demonstrated that NMN could alleviate heat stress in bovine mammary epithelial cells by reducing ER stress and minimizing mitochondrial damage [19]. Moreover, the balance of mitochondrial fusion and fission is crucial for maintaining mitochondrial quality [19] and an imbalance in this balance can result in a decline in oocyte quality [53,54]. The positive regulation of mitochondrial fusion in mammals is achieved through the genes MFN1 and MFN2 [55], while mitochondrial fission is linked to the genes DRP1 and FIS1 [56], and inhibition of these genes can inhibit mitochondrial fission [57].…”

Section: Discussionsupporting

confidence: 91%

Effects of β-Nicotinamide Mononucleotide, Berberine, and Cordycepin on Lipid Droplet Content and Developmental Ability of Vitrified Bovine Oocytes

Yang

Zhang

et al. 2023

Antioxidants

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Oocyte vitrification is crucial for livestock reproduction, germplasm conservation, and human-assisted reproduction, but the overabundance of lipids is highly detrimental to oocyte development. It is necessary to reduce the lipid droplet content of oocytes before cryopreservation. This study analyzed the impact of β-nicotinamide mononucleotide (NMN), berberine (BER), or cordycepin (COR) on various aspects of bovine oocytes, including lipid droplet content and the expression levels of genes related to lipid synthesis in bovine oocytes, development ability, reactive oxygen species (ROS), apoptosis, and the expression levels of genes associated with endoplasmic reticulum (ER) stress, and mitochondrial function in vitrified bovine oocytes. The results of our study indicated that 1 μM NMN, 2.5 μM BER, and 1 μM COR were effective in reducing the lipid droplet content and suppressing the expression levels of genes involved in lipid synthesis in bovine oocytes. Our findings showed that the vitrified bovine oocytes treated with 1 μM of NMN had a significantly higher survival rate and better development ability compared to the other vitrified groups. Additionally, 1 μM NMN, 2.5 μM BER, and 1 μM COR decreased the levels of ROS and apoptosis, decreased the mRNA expression levels of genes involved in ER stress and mitochondrial fission but increased the mRNA expression levels of genes associated with mitochondrial fusion in the vitrified bovine oocytes. Our study results suggested that 1 μM NMN, 2.5 μM BER, and 1 μM COR effectively decreased the lipid droplet content and enhanced the development ability of vitrified bovine oocytes by lowering ROS levels, reducing ER stress, regulating mitochondrial function, and inhibiting apoptosis. Furthermore, the results showed that 1 μM NMN was more effective than 2.5 μM BER and 1 μM COR.

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Section: Discussionsupporting

confidence: 91%

Effects of β-Nicotinamide Mononucleotide, Berberine, and Cordycepin on Lipid Droplet Content and Developmental Ability of Vitrified Bovine Oocytes

Yang

Zhang

et al. 2023

Antioxidants

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show abstract

“…3 D line 1, Q), suggesting an up-regulation of lipid peroxidation in oocytes. Beyond expectation, the key antioxidant molecule mitochondrial superoxide dismutase (SOD2) [ 33 ] was up-regulated while GPX4 and Peroxiredoxin (PRX) [ 28 ] were not changed much in aging oocytes ( Fig. 3 D line 2–4, R- T), it showed that the cellular antioxidant capacity was still not exhausted by ROS.…”

Section: Resultsmentioning

confidence: 99%

“…The slides were air-dried at room temperature and stored at −20 °C before use. Prior to 1 h blocking in 1 % BSA, slides were immersed in adequate PBS to wash off salts mixed in the chromosome samples [ 28 , 29 ]. The samples were immune-stained with human centromere auto serum (CREST) (1:100; 90C-CS1058, Fitzgerald) and counterstained with mounting medium containing DAPI, then analyzed with ZEISS microscopic system and Image J software as stated above.…”

Section: Methodsmentioning

confidence: 99%

Iron accumulation in ovarian microenvironment damages the local redox balance and oocyte quality in aging mice

Chen,

Zhang,

Tian

et al. 2024

Redox Biology

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Mitochondrial Quality Control in Ovarian Function: From Mechanisms to Therapeutic Strategies

Wang,

Liu,

Wang

et al. 2024

Reprod. Sci.

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Adverse effects of 2-Methoxyestradiol on mouse oocytes during reproductive aging

Cited by 5 publications

References 44 publications

Effects of β-Nicotinamide Mononucleotide, Berberine, and Cordycepin on Lipid Droplet Content and Developmental Ability of Vitrified Bovine Oocytes

Effects of β-Nicotinamide Mononucleotide, Berberine, and Cordycepin on Lipid Droplet Content and Developmental Ability of Vitrified Bovine Oocytes

Iron accumulation in ovarian microenvironment damages the local redox balance and oocyte quality in aging mice

Mitochondrial Quality Control in Ovarian Function: From Mechanisms to Therapeutic Strategies

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