AedesCAPA-PVK-1 displays diuretic and dose dependent antidiuretic potential in the larval mosquito Aedes aegypti (Liverpool)

Ionescu, Adrian; Donini, Andrew

doi:10.1016/j.jinsphys.2012.07.002

Cited by 22 publications

(55 citation statements)

References 51 publications

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“…This is a similar pattern that has been described for D. melanogaster (Kean et al 2002) and in the moth M. sexta (Loi and Tublitz 2004). In mosquitoes the PVKs are involved in regulating water balance as they are in D. melanogaster (Pollock et al 2004;Ionescu and Donini 2012). The function of the PK/PBAN peptides is unknown in mosquitoes.…”

Section: Discussionsupporting

confidence: 71%

Pyrokinin/PBAN-like peptides in the central nervous system of mosquitoes

2014

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The pyrokinin/pheromone biosynthesis activating neuropeptide (PBAN) family of peptides is characterized by a common C-terminal pentapeptide, FXPRLamide, which is required for diverse physiological functions in various insects. Polyclonal antisera against the C-terminus was utilized to determine the location of cell bodies and axons in the central nervous systems of larval and adult mosquitoes. Immunoreactive material was detected in three groups of neurons in the subesophageal ganglion of larvae and adults. The corpora cardiaca of both larvae and adults contained immunoreactivity indicating potential release into circulation. The adult and larval brains had at least one pair of immunoreactive neurons in the protocerebrum with the adult brain having additional immunoreactive neurons in the dorsal medial part of the protocerebrum. The ventral ganglia of both larvae and adults each contained one pair of neurons that sent their axons to a perisympathetic organ associated with each abdominal ganglion. These results indicate that the mosquito nervous system contains pyrokinin/PBAN-like peptides and that these peptides could be released into the hemolymph. The peptides in insects and mosquitoes are produced by two genes, capa and pk/pban. Utilizing PCR protocols, we demonstrate that products of the capa gene could be produced in the abdominal ventral ganglia and the products of the pk/pban gene could be produced in the subesophageal ganglion. Two receptors for pyrokinin peptides were differentially localized to various tissues. Abstract Pyrokinin/pheromone biosynthesis activating neuropeptide (PBAN) family of peptides is characterized by a common C-terminal pentapeptide, FXPRLamide, that is required for diverse physiological functions in various insects. Polyclonal antisera against the C-terminus was utilized to determine the location of cell bodies and axons in the central nervous systems of larval and adult mosquitoes. Immunoreactive material was detected in three groups of neurons in the subesophageal ganglion of larvae and adults. The corpora cardiaca of both larvae and adults contained immunoreactivity indicating potential release into circulation. The adult and larval brains had at least one pair of immunoreactive neurons in the protocerebrum with the adult brain having additional immunoreactive neurons in the dorsal medial part of the protocerebrum. The ventral ganglia of both larvae and adults each contained one pair of neurons that sent their axons to a perisympathetic organ associated with each abdominal ganglion. These results indicate that the mosquito nervous system contains pyrokinin/PBAN-like peptides and that these peptides could be released into the hemolymph. The peptides in insects and mosquitoes are produced by two genes, capa and pk/pban. Utilizing PCR protocols we demonstrate that products of the capa gene could be produced in the abdominal ventral ganglia and the products of the pk/pban gene could be produced in the subesophageal ganglion. Two receptors for pyrokinin peptides were differ...

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Section: Discussionsupporting

confidence: 71%

Pyrokinin/PBAN-like peptides in the central nervous system of mosquitoes

2014

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“…Capa neuropeptides have a diuretic effect on the Malpighian tubules of Drosophila (Pollock et al, 2004), but in R. prolixus and other insects they act antidiuretic (Coast and Garside, 2005; Paluzzi and Orchard, 2006). Recently, it was shown that the Aedes capa neuropeptide can induce either diuretic or antidiuretic effects depending on the dose (Ionescu and Donini, 2012). In addition, capa neuropeptides have myotropic effects in a variety of insects (Wegener et al, 2002; Predel and Wegener, 2006).…”

Section: Deorphanized Neuropeptide Receptorsmentioning

confidence: 99%

More than two decades of research on insect neuropeptide GPCRs: an overview

Caers¹,

Verlinden²,

Zels³

et al. 2012

Front. Endocrin.

155

143

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This review focuses on the state of the art on neuropeptide receptors in insects. Most of these receptors are G protein-coupled receptors (GPCRs) and are involved in the regulation of virtually all physiological processes during an insect's life. More than 20 years ago a milestone in invertebrate endocrinology was achieved with the characterization of the first insect neuropeptide receptor, i.e., the Drosophila tachykinin-like receptor. However, it took until the release of the Drosophila genome in 2000 that research on neuropeptide receptors boosted. In the last decade a plethora of genomic information of other insect species also became available, leading to a better insight in the functions and evolution of the neuropeptide signaling systems and their intracellular pathways. It became clear that some of these systems are conserved among all insect species, indicating that they fulfill crucial roles in their physiological processes. Meanwhile, other signaling systems seem to be lost in several insect orders or species, suggesting that their actions were superfluous in those insects, or that other neuropeptides have taken over their functions. It is striking that the deorphanization of neuropeptide GPCRs gets much attention, but the subsequent unraveling of the intracellular pathways they elicit, or their physiological functions are often hardly examined. Especially in insects besides Drosophila this information is scarce if not absent. And although great progress made in characterizing neuropeptide signaling systems, even in Drosophila several predicted neuropeptide receptors remain orphan, awaiting for their endogenous ligand to be determined. The present review gives a précis of the insect neuropeptide receptor research of the last two decades. But it has to be emphasized that the work done so far is only the tip of the iceberg and our comprehensive understanding of these important signaling systems will still increase substantially in the coming years.

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“…The only functional data in mosquitoes show that PK2a from Ae. aegypti inhibits fluid secretion in larval Malpighian tubules at femtomolar concentration but stimulates diuresis at higher concentrations (Ionescu and Donini, 2012). By previous nomenclature PK2a would be referred to as CAPA1 (Jurenka, 2015), which is inconsistent with its diuretic activity.…”

Section: Peptide Hormone Functions In Mosquitoesmentioning

confidence: 99%

Mosquito Peptide Hormones

Strand

Brown

Vogel

2016

Advances in Insect Physiology

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Mosquitoes, like other insects, produce a diversity of peptide hormones that are processed from different precursor proteins and have a range of activities. Early studies relied on purification of bioactive peptides for hormone identification, but more recently genomic data have provided the information needed to more comprehensively identify peptide hormone genes and associated receptors. The first part of this chapter summarizes the known or predicted peptide hormones that are produced by mosquitoes. The second part of this chapter discusses the sources of these molecules and their functions.

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AedesCAPA-PVK-1 displays diuretic and dose dependent antidiuretic potential in the larval mosquito Aedes aegypti (Liverpool)

Cited by 22 publications

References 51 publications

Pyrokinin/PBAN-like peptides in the central nervous system of mosquitoes

Pyrokinin/PBAN-like peptides in the central nervous system of mosquitoes

More than two decades of research on insect neuropeptide GPCRs: an overview

Mosquito Peptide Hormones

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