Aegilops sharonensis genome-assisted identification of stem rust resistance gene Sr62

Yu, Guotai; Matny, Oadi; Champouret, Nicolas; Steuernagel, Burkhard; Moscou, Matthew J.; Hernández-Pinzón, Inmaculada; Green, Phon; Hayta, Şadiye; Smedley, Mark; Harwood, Wendy; Kangara, Ngonidzashe; Yue, Yajuan; Gardener, Catherine; Banfield, Mark J.; Olivera, Pablo D.; Welchin, Cole; Simmons, Jamie; Millet, E.; Minz‐Dub, Anna; Ronen, Moshe; Avni, Raz; Sharon, Amir; Patpour, Mehran; Justesen, Annemarie Fejer; Jayakodi, Murukarthick; Himmelbach, Axel; Stein, Nils; Wu, Shuangye; Poland, Jesse; Ens, Jennifer; Pozniak, Curtis; Karafiátová, Miroslava; Molnár, István; Doležel, Jaroslav; Ward, Eric; Reuber, T. Lynne; Jones, Jonathan D. G.; Mascher, Martin; Steffenson, Brian J.; Wulff, Brande B. H.

doi:10.1038/s41467-022-29132-8

Cited by 79 publications

(89 citation statements)

References 111 publications

(154 reference statements)

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“…These tandem kinase genes include Rpg1, Yr15, Sr60, Sr62, Pm24, WTK4, and Rwt4 (refs. [7][8][9][10][11][12][13] ). Other resistance genes offer some variation to this architecture with protein kinases fused to a steroidogenic acute regulatory protein-related lipid transfer domain (Yr36) 14 , a C2 domain and a multi-transmembrane region (Pm4) 15 , a major sperm protein (Snn3) 16 , an NLR (Tsn1, Rpg5, and Sm1) 17,18,19 , and a von Willebrand factor type A domain (Lr9) 20 .…”

Section: Mainmentioning

confidence: 99%

“…We calculated the probability of this being the candidate gene using formula number 4 developed by Yu et al 2022 (ref. 10 ), with 2598 bp of Sr43 coding sequence (CDS), assuming the average gene CDS is 1000 bp in length, and that chromosome 7D has 5822 genes. All identi ed mutations were G-to-A or C-to-T transition mutations, which are typical of EMS mutagenesis.…”

Section: Candidate Gene Identi Cationmentioning

confidence: 99%

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The wheat stem rust resistance gene Sr43 encodes an unusual protein kinase

Matny

Gourdoupis

et al. 2022

Preprint

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To safeguard bread wheat against pests and diseases, breeders have introduced over 200 resistance genes into its genome, thus nearly doubling the number of designated resistance genes in the wheat gene pool. Isolating these genes facilitates their fast-tracking in breeding programs and incorporation into polygene stacks for more durable resistance. We cloned the stem rust resistance gene Sr43, which was crossed into bread wheat from the wild grass Thinopyrum elongatum. Sr43 encodes a protein kinase fused to two domains of unknown function. The gene, which is unique to the Triticeae, appears to have arisen through a gene fusion event 6.7 to 11.6 million years ago. Transgenic expression of Sr43 in wheat conferred high levels of resistance to a wide range of isolates of the pathogen causing stem rust, highlighting the potential value of Sr43 in resistance breeding and engineering.

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Section: Mainmentioning

confidence: 99%

Section: Candidate Gene Identi Cationmentioning

confidence: 99%

The wheat stem rust resistance gene Sr43 encodes an unusual protein kinase

Matny

Gourdoupis

et al. 2022

Preprint

View full text Add to dashboard Cite

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“…Sr43 contains a kinase domain fused to two domains of unknown function that might serve as decoys. Together, these two studies highlight the emerging role of unusual kinase fusion proteins in wheat disease resistance [13][14][15][16][17][18][44][45][46][47] , which will form the basis to utilize this protein class in breeding. (128 F2 plants), respectively.…”

mentioning

confidence: 99%

“…1d). Wheat tandem kinases (WTKs) have recently emerged as a new prominent player involved in disease resistance in Triticeae, the botanical tribe including wheat and barley [12][13][14][15][16][17][18] . The fusion of a WTK to another domain, however, is unique and highly unusual.…”

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confidence: 99%

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An unusual tandem kinase fusion protein confers leaf rust resistance in wheat

Wang

Abrouk

Gourdoupis

et al. 2022

Preprint

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The introgression of chromosome segments from wild relatives is an established strategy to enrich crop germplasm with novel disease resistance genes. Here, we use mutagenesis and transcriptome sequencing to clone the leaf rust resistance gene Lr9, which was introduced into bread wheat from the wild grass species Aegilops umbellulata. We establish that Lr9 encodes an unusual tandem kinase fusion protein. Long-read sequencing of a wheat Lr9 introgression line and the putative Ae. umbellulata Lr9 donor enabled us to assemble the ~28.4-Mb Lr9 translocation and to identify the translocation breakpoint. We likewise cloned Lr58, which was reportedly introgressed from Ae. triuncialis, but has an identical coding sequence compared to Lr9. Cytogenetic and haplotype analyses corroborate that the two genes originate from the same translocation event. Our work sheds light on the emerging role of kinase fusion proteins in wheat disease resistance, expanding the repertoire of disease resistance genes for breeding.

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Rapid Cloning of Disease Resistance Genes in Wheat

Running,

Faris

2023

Compendium of Plant Genomes

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Wheat is challenged by rapidly evolving pathogen populations, resulting in yield losses. Plants use innate immune systems involving the recognition of pathogen effectors and subsequent activation of defense responses to respond to pathogen infections. Understanding the genes, genetic networks, and mechanisms governing plant-pathogen interactions is key to the development of varieties with robust resistance whether through conventional breeding techniques coupled with marker selection, gene editing, or other novel strategies. With regards to plant-pathogen interactions, the most useful targets for crop improvement are the plant genes responsible for pathogen effector recognition, referred to as resistance (R) or susceptibility (S) genes, because they govern the plant’s defense response. Historically, the molecular identification of R/S genes in wheat has been extremely difficult due to the large and repetitive nature of the wheat genome. However, recent advances in gene cloning methods that exploit reduced representation sequencing methods to reduce genome complexity have greatly expedited R/S gene cloning in wheat. Such rapid cloning methods referred to as MutRenSeq, AgRenSeq, k-mer GWAS, and MutChromSeq allow the identification of candidate genes without the development and screening of high-resolution mapping populations, which is a highly laborious step often required in traditional positional cloning methods. These new cloning methods can now be coupled with a wide range of wheat genome assemblies, additional genomic resources such as TILLING populations, and advances in bioinformatics and data analysis, to revolutionize the gene cloning landscape for wheat. Today, 58 R/S genes have been identified with 42 of them having been identified in the past six years alone. Thus, wheat researchers now have the means to enhance global food security through the discovery of R/S genes, paving the way for rapid R gene deployment or S gene elimination, manipulation through gene editing, and understanding wheat-pathogen interactions at the molecular level to guard against crop losses due to pathogens.

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Aegilops sharonensis genome-assisted identification of stem rust resistance gene Sr62

Cited by 79 publications

References 111 publications

The wheat stem rust resistance gene Sr43 encodes an unusual protein kinase

The wheat stem rust resistance gene Sr43 encodes an unusual protein kinase

An unusual tandem kinase fusion protein confers leaf rust resistance in wheat

Rapid Cloning of Disease Resistance Genes in Wheat

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