Aerobic and anaerobic enrichment cultures highlight the pivotal role of facultative anaerobes in soil hydrocarbon degradation

Cason, Errol D.; Vermeulen, Jan‐G; Müller, Walter Joseph; Heerden, Esta van; Valverde, Ángel

doi:10.1080/10934529.2018.1558902

Cited by 10 publications

(6 citation statements)

References 55 publications

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“…Until the present study, there had been no published information on the ability of microorganisms of the genera Stenotrophomonas , Psychrobacter , Micrococcus and Peribacillus to oxidize oil hydrocarbons under aerobic and anaerobic conditions. For the other genera tested in this study, Rhodococcus, Pseudomonas and Bacillus , such an ability has already been described [ 13 , 23 , 39 , 40 ].…”

Section: Resultsmentioning

confidence: 54%

“…The main methods in the field of bioindication at present are the detection of functional genes involved in the biodegradation of hydrocarbons and the description of properties of bioindicator species [ 13 , 19 , 20 , 21 , 22 , 23 , 24 ]. Due to the plasticity of bioindicator methods, we assume that they can be used not only for the detection of oil and gas deposits but also for more detailed descriptions of such deposits, for example, for the identification of traditional and gas-hydrate deposits, without direct access to the surface of gas hydrates [ 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

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Groups of Geomicrobiological Indicators Are Spread across Gas-Hydrate and Non-Gas-Hydrate Areas in the Northern Part of the Sea of Japan

Пономарева

Eskova

Shakirov

et al. 2022

Biology

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The bioindication of oil and gas fields is a field of geomicrobiology that is mainly devoted to the detection of hydrocarbon-oxidizing microbial indicator species or functional genes in total DNA. However, it appears promising to use the physiological properties of microorganisms detection deposit type of hydrocarbons, in particular their ability to oxidize hydrocarbons under aerobic and anaerobic conditions. In this study, the most promising approach in this area was the method used for assessing the anaerobic degradation of hydrocarbons. When comparing molecular genetics and cultured methods of bioindication, it can be concluded that molecular biomarkers of functional genes for the anaerobic destruction of hydrocarbons (masD) make it possible to separate areas with traditional and gas-hydrate types of deposits. Using cultured methods, we found that representatives of the Nocardiaceae family of the phylum Actinomycetota were tied to the areas where gas hydrates were found. The ability of aerobic and facultative anaerobic hydrocarbon-oxidizing microorganisms to anaerobically utilize hydrocarbons was determined with cultured methods. For the first time, this ability was revealed for the genera Stenotrophomonas, Psychrobacter, Micrococcus and Peribacillus. The wide distribution of this ability that we found in strains isolated from both study regions suggests its prominent role in the destruction of hydrocarbons in marine sediments.

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Section: Resultsmentioning

confidence: 54%

Section: Introductionmentioning

confidence: 99%

Groups of Geomicrobiological Indicators Are Spread across Gas-Hydrate and Non-Gas-Hydrate Areas in the Northern Part of the Sea of Japan

Пономарева

Eskova

Shakirov

et al. 2022

Biology

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“…Thus, they contribute to the creation of biogeochemical gradients, such as redox gradients in sediments and water columns ( Acosta-González and Marqués, 2016 ). Facultative bacteria are abundant in soil ( Table S1 in Supplementary material ), playing a crucial role in organic matter decomposition, element cycling, and the transformation of various compounds under changing oxygen conditions ( McNally et al, 1998 ; Grishchenkov et al, 2000 ; Cason et al, 2019 ; Zuo et al, 2021 ; Sheng et al, 2021a ; Dong et al, 2023a ). In anaerobic environments, such as wetlands and sediments, facultative anaerobes can make up a substantial proportion of the microbial community ( Bai et al, 2000 ; Zhu et al, 2011 ).…”

Section: Discussionmentioning

confidence: 99%

“…Facultative anaerobic bacteria exhibit higher adaptability to environmental changes compared to obligate bacteria ( Cason et al, 2019 ). Several studies have shown that facultative anaerobes have the capability to biodegrade specific PAH compounds, such as phenanthrene, pyrene, and benzo[ a ]pyrene (BaP), under both aerobic and Fe(III) reduction conditions ( Liang et al, 2014 ; Yan et al, 2017 ; Zhang et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Differential degradation of petroleum hydrocarbons by Shewanella putrefaciens under aerobic and anaerobic conditions

Li,

Liu,

Guo

et al. 2024

Front. Microbiol.

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The complexity of crude oil composition, combined with the fluctuating oxygen level in contaminated environments, poses challenges for the bioremediation of oil pollutants, because of compound-specific microbial degradation of petroleum hydrocarbons under certain conditions. As a result, facultative bacteria capable of breaking down petroleum hydrocarbons under both aerobic and anaerobic conditions are presumably effective, however, this hypothesis has not been directly tested. In the current investigation, Shewanella putrefaciens CN32, a facultative anaerobic bacterium, was used to degrade petroleum hydrocarbons aerobically (using O2 as an electron acceptor) and anaerobically (using Fe(III) as an electron acceptor). Under aerobic conditions, CN32 degraded more saturates (65.65 ± 0.01%) than aromatics (43.86 ± 0.03%), with the following order of degradation: dibenzofurans > n-alkanes > biphenyls > fluorenes > naphthalenes > alkylcyclohexanes > dibenzothiophenes > phenanthrenes. In contrast, under anaerobic conditions, CN32 exhibited a higher degradation of aromatics (53.94 ± 0.02%) than saturates (23.36 ± 0.01%), with the following order of degradation: dibenzofurans > fluorenes > biphenyls > naphthalenes > dibenzothiophenes > phenanthrenes > n-alkanes > alkylcyclohexanes. The upregulation of 4-hydroxy-3-polyprenylbenzoate decarboxylase (ubiD), which plays a crucial role in breaking down resistant aromatic compounds, was correlated with the anaerobic degradation of aromatics. At the molecular level, CN32 exhibited a higher efficiency in degrading n-alkanes with low and high carbon numbers relative to those with medium carbon chain lengths. In addition, the degradation of polycyclic aromatic hydrocarbons (PAHs) under both aerobic and anaerobic conditions became increasingly difficult with increased numbers of benzene rings and methyl groups. This study offers a potential solution for the development of targeted remediation of pollutants under oscillating redox conditions.

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“…Biodegradation of hydrocarbons in water has been shown to occur under aerobic and anaerobic conditions, with the highest hydrocarbon reduction observed in aerobic conditions [14]. Also, microorganisms such as Bacillus, Citrobacter, and Pseudomonas, which partake in aerobic and anaerobic degradation of hydrocarbons are facultative anaerobes [15][16][17][18]. Products of anaerobic biodegradation of hydrocarbons include volatile and long-chain fatty acids, e.g.…”

Section: Reduction In Hydrocarbon Concentration In the Bioremediation Setupsmentioning

confidence: 99%

Effect of Aerating Duration on Hydrocarbon Biodegradation in a Simulated Crude-Oil Polluted Aquatic Environment Undergoing Bioremediation

Ewoh

Peekate

2021

SAJRM

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The aim of this research work was to determine the aerating duration that would be effective in enhancing hydrocarbon biodegradation rate during bioremediation of crude-oil polluted river. Sediment and river-water were placed in four glass troughs labeled CT (control), A, B, and C. The setups were polluted with crude-oil, and allowed undisturbed for 2 weeks. Subsequently, accessible crude-oil on the surface was removed; bacteria and nutrients were then added. Air was bubbled for 3 hours into setups A, B, and C, at daily, 3 days, and 7 days interval respectively. Aeration was not applied to setup CT. On day 1, 7, 14, and 21, hydrocarbon concentration was determined; populations of total heterotrophic bacteria (THB) and hydrocarbon-utilizing bacteria (HUB) were also determined. The time it will take for hydrocarbons in the setups to biodegraded “completely” was calculated using first-order reaction equation. The results obtained showed that 71.43, 86.39, 83.17, and 15.42 % hydrocarbon degradation were obtained in setup A, B, C, and CT respectively. The time it will take for hydrocarbons in the setups to biodegrade “completely” were 129, 89, 101, and 1079 days for A, B, C, and CT respectively. There was slight reduction in population of HUB in setup CT, fairly stable population in setup A, and increase in population of HUB in setups B and C. It is concluded that aerating crude-oil polluted aquatic environment for 3 hours at 3 days interval will be more effective in enhancing hydrocarbon biodegradation rate during bioremediation.

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Aerobic and anaerobic enrichment cultures highlight the pivotal role of facultative anaerobes in soil hydrocarbon degradation

Cited by 10 publications

References 55 publications

Groups of Geomicrobiological Indicators Are Spread across Gas-Hydrate and Non-Gas-Hydrate Areas in the Northern Part of the Sea of Japan

Groups of Geomicrobiological Indicators Are Spread across Gas-Hydrate and Non-Gas-Hydrate Areas in the Northern Part of the Sea of Japan

Differential degradation of petroleum hydrocarbons by Shewanella putrefaciens under aerobic and anaerobic conditions

Effect of Aerating Duration on Hydrocarbon Biodegradation in a Simulated Crude-Oil Polluted Aquatic Environment Undergoing Bioremediation

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