Aerobic Decolorization and Detoxification of a Disperse Dye in Textile Effluent by a New Isolate ofBacillus sp.

Abstract

A number of aerobic species capable of decolorizing some of the dyes in a textile mill effluent were isolated. One of the isolates was able to decolorize Terasil black dye under aerobic conditions in the presence of an exogenous carbon source after 5 days. Glucose or starch (%1 ea) are essential for decolorization but the process proceeds faster in the presence of 0.5% yeast extract. Results of the BOD(5) show that the untreated effluent samples have a low BOD value, whereas treated samples show an initial increase in BOD up to 15 days followed by a decrease after 20 days. FT-IR and GC-MS data also reveal that the initial components in the untreated effluent disappear after 20 days of treatment, confirming biodegradation of the dye. Phytotoxicity tests on the untreated effluent samples using the seeds of Lens orientalis, Triticum aestivum, and Triticum boeoticum indicate that the first one is the most sensitive while the last one is the most resistant. On the other hand the treated effluent allows 90% germination in Triticum boeoticum seeds and 100% germination in the other two.

Decolorization of textile plant effluent by Citrobacter sp. strain KCTC 18061P

“…Our data revealed that the addition of 1% glucose markedly improved the color removal efficiency of textile dye effluent. This is consistent with the previous report (Pourbabaee et al, 2006) that showed a 60% increase of dye decolorization rate in dyeing mill effluent, but it is noted that nitrogen sources tested in this study did not affect the color removal efficiency of textile dye effluent. On the other hand, it is probably impossible to completely decolorize wastewater from textile plant with a single strain, because dye effluent from the textile industry contains a variety of polluting substances including mixtures of different dyestuffs ).…”

“…was 56% (Sani and Banerjee, 1999). Pourbabaee et al (2006) recently reported that the maximum reduction of COD in real dyeing mill effluent by Bacillus sp. was 65%, while in the present study its maximum reduction in dye effluent from textile plant by Citrobacter sp.…”

“…Decolorization of textile dye effluent by fungi is well known to occur though dye adsorption or biodegradation through an enzyme system, such as laccase, manganese peroxidase, and lignin peroxidase Fu and Viraraghavan, 2001;Robinson et al, 2001;Wesenberg et al, 2003). However, to our knowledge, few studies on its decolorization using a single bacterial strain have been reported (Pourbabaee et al, 2006;Sani and Banerjee, 1999), although decolorization of textile dye effluent by mixed bacterial cultures has been reported (Kim et al, 2003;Nigam et al, 1996). It was reported that the reduction of COD in textile and dye-stuff effluent by Kurthia sp.…”

Evaluation of Microbial Systems for Biotreatment of Textile Waste Effluents in Nigeria: Biodecolourization and Biodegradation of textile Dye

“…Nevertheless, Aspergillus niger had relatively higher % COD and BOD reductions than other bacterial and fungal isolates. Pourbabaee et al (2006) and Andleeb et al (2010) observed similar reductions in COD and BOD in their study of aerobic biotreatment of Terasil black in textile effluent by a newly isolated Bacillus sp. and sulfur black by Aspergillus terreus SA 3, respectively.…”

“…A great number of these fungi (especially white-rot fungi) have been shown to excrete extracellular enzymes like lignin peroxidase, manganese peroxidase and laccase (Hatakka, 1994) which catalyze the formation of activated oxygen so that the process of attack on the stable structure of dyes can be initiated. The role of some bacterial and algal species for the decolourization and degradation of textile dyes has also been reported (Jumarkar et al, 2006;Olukanni et al, 2006;Pourbabaee et al, 2006;Togo et al, 2008;Cheriaa et al, 2009). Dyes are removed by biosorption (Fu and Viraraghavan, 2000) and enzymatic mineralization (degradation) by lignin peroxidase, manganese peroxidase, manganese independent peroxidase and laccases (Raaghukumar et al, 1996;Duran et al, 2002;Wesenberg et al, 2003;Svobodova et al, 2006).…”