Aerobic denitrification affects gaseous nitrogen loss in biofloc-based recirculating aquaculture system

Deng, Min; Li, Lü; Dai, Zhili; Yeerken, Senbati; Song, Kang; He, Xinyi

doi:10.1016/j.aquaculture.2020.735686

Cited by 55 publications

(22 citation statements)

References 54 publications

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“…The main difference was related to the abundance of the genus Flavobacterium , which was not obtained in the enrichment cultures. Species of Flavobacterium are generally aerobic heterotrophs, many of which are psychrotolerant [ 62 ], but the genus also harbors aerobic and facultative anaerobic denitrifiers, thus demonstrating metabolic versatility [ 63 , 64 , 65 ]. The genus was also found in relatively high abundance in previous studies of denitrifiers from wastewater treatment facilities [ 66 ] and bioreactors [ 19 , 47 ].…”

Section: Discussionmentioning

confidence: 99%

Temperature Sensitivity and Composition of Nitrate-Reducing Microbiomes from a Full-Scale Woodchip Bioreactor Treating Agricultural Drainage Water

et al. 2021

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Denitrifying woodchip bioreactors (WBR), which aim to reduce nitrate (NO3−) pollution from agricultural drainage water, are less efficient when cold temperatures slow down the microbial transformation processes. Conducting bioaugmentation could potentially increase the NO3− removal efficiency during these specific periods. First, it is necessary to investigate denitrifying microbial populations in these facilities and understand their temperature responses. We hypothesized that seasonal changes and subsequent adaptations of microbial populations would allow for enrichment of cold-adapted denitrifying bacterial populations with potential use for bioaugmentation. Woodchip material was sampled from an operating WBR during spring, fall, and winter and used for enrichments of denitrifiers that were characterized by studies of metagenomics and temperature dependence of NO3− depletion. The successful enrichment of psychrotolerant denitrifiers was supported by the differences in temperature response, with the apparent domination of the phylum Proteobacteria and the genus Pseudomonas. The enrichments were found to have different microbiomes’ composition and they mainly differed with native woodchip microbiomes by a lower abundance of the genus Flavobacterium. Overall, the performance and composition of the enriched denitrifying population from the WBR microbiome indicated a potential for efficient NO3− removal at cold temperatures that could be stimulated by the addition of selected cold-adapted denitrifying bacteria.

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Section: Discussionmentioning

confidence: 99%

Temperature Sensitivity and Composition of Nitrate-Reducing Microbiomes from a Full-Scale Woodchip Bioreactor Treating Agricultural Drainage Water

et al. 2021

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“…RDA represented the same conclusion that these pioneers have negative correlation with shear stress while a presented positive relationship with the zeta potential of the carrier and biofilm (Figure d) due to the paramount importance of electrostatic interactions in the regulation of biofilm formation in the first step of adhesion to surfaces and Flavobacterium is an aerobic denitrifier . In addition, it was worth mentioning that unclassified_f__Enterobacteriaceae , a kind of heterotrophic denitrifying bacterium, was found to be predominant in glucose-fed microbial fuel cells; hence, aerobic denitrifying bacteria involved in electrostatic interactions have more potential possibility to colonize the habitat faster.…”

Section: Resultsmentioning

confidence: 55%

“…61 Bacteriovorax is a Gram-negative, aerobic, and obligate predator of Gram-negative bacteria 62 and Flavobacterium is an aerobic denitrifier. 63 In addition, it was worth mentioning that unclassif ied_f__Enterobacteriaceae, a kind of heterotrophic denitrifying bacterium, 64 was found to be predominant in glucose-fed microbial fuel cells; 65 hence, aerobic denitrifying bacteria involved in electrostatic interactions have more potential possibility to colonize the habitat faster.…”

Section: Methodsmentioning

confidence: 99%

Revisiting the Microscopic Processes of Biofilm Formation on Organic Carriers: A Study under Variational Shear Stresses

Fan

Zhu

Zhang

et al. 2021

ACS Appl. Bio Mater.

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The microscopic process of biofilm development on carriers is critical for interfacial regulation of biofilms in attached-growth wastewater treatment. However, the process under shear stress has not been well understood. The study purposed to revisit the processes of biofilm formation on organic carriers under different shear stresses with special highlights on bacterial reversible adhesion and pioneers in the microbial community. Biofilm formation on high-density polyethylene, polyamide, acrylonitrile butadiene styrene plastic, polyvinyl chloride, and polycarbonate carriers under shear stresses ranging from 1.0 to 2.5 Pa was investigated using Couette–Taylor reactors. Employing extended Derjaguin–Landau–Verwey–Overbeek (XDLVO) theory, the bacterial reversible adhesion regions ranging from 3.74 ± 0.20 to 5.51 ± 0.24 nm on an organic carrier were quantified for the first time, elucidating significant differences among different carriers (p < 0.01). The colonization of pioneers in the microbial community was significantly altered by shear stress rather than carrier properties (p < 0.01). In particular, the diversity of the biofilm microbial community was pronouncedly enhanced by a higher shear stress (p < 0.01). XDLVO analysis suggested that extracellular polymeric substances had a negative feedback on subsequent microbial adhesion and biofilm development, especially the transition from reversible to irreversible bacterial adhesion. This study contributed to a better understanding of the biofilm formation process at the microscopic scale and shed light on micro-interfacial manipulation for biofilm accumulation or renewal.

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“…Although accumulation of nitrite and nitrate was common in biofloc systems (Tierney & Ray, 2018), there should widely occur in aerobic denitrification (Deng, Li et al, 2020). In this study, the abundance of the denitrifying bacteria in all the treatments varied between 32.86% and 68.58% on day 26 and between 10.60% and 32.42% on day 40, which in the ND12C treatment were higher than the other treatments on two time points.…”

Section: Discussionmentioning

confidence: 99%

Effect of carbon source and C/N ratio on microbial community and function in ex situ biofloc system with inoculation of nitrifiers and aerobic denitrifying bacteria

Qiu

Liao

Wang

et al. 2021

Aquaculture Research

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To control the inorganic nitrogen and biofloc concentrations in the biofloc system, it is necessary to intensify the nitrogen removal by nitrification and denitrification. In this study, nitrifiers and aerobic denitrifying bacteria were inoculated into aquaculture wastewater, with 16.80 mg/L of nitrogen input per day after start‐up, and by addition of glucose and citrate C/N to 9 and 12, without adding carbon source as control (C/N 6.17). The concentrations of inorganic nitrogen, total nitrogen and biofloc, and the diversity of the microbial community were investigated during the experimental period of 40 days. The results showed that 84.17–89.97% of nitrogen input was removed in all the treatments, residual nitrogen was higher in the glucose treatments. Lower inorganic nitrogen at a higher C/N ratio was obtained with the addition of the same carbon source due to more assimilation. Among all, the citrate treatment at C/N 12 obtained the best control in inorganic nitrogen concentrations and better in biofloc concentration owing to efficient dissimilation and assimilation. Moreover, from day 26 to day 40, the number of detected OTUs increased by 22.90–58.40% while the Shannon indices have not changed much in all the treatments. Ten of the top 15 genera were the typical denitrifying genera in each treatment, the total abundance of these decreased by 7.00% to 58.00% while that of Paracoccus, as one of the denitrifiers added, increased by 3.00% to 25.00%. This suggested that the addition of citrate at higher C/N could improve the denitrification function of biofloc system.

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Aerobic denitrification affects gaseous nitrogen loss in biofloc-based recirculating aquaculture system

Cited by 55 publications

References 54 publications

Temperature Sensitivity and Composition of Nitrate-Reducing Microbiomes from a Full-Scale Woodchip Bioreactor Treating Agricultural Drainage Water

Temperature Sensitivity and Composition of Nitrate-Reducing Microbiomes from a Full-Scale Woodchip Bioreactor Treating Agricultural Drainage Water

Revisiting the Microscopic Processes of Biofilm Formation on Organic Carriers: A Study under Variational Shear Stresses

Effect of carbon source and C/N ratio on microbial community and function in ex situ biofloc system with inoculation of nitrifiers and aerobic denitrifying bacteria

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