Aerobic Dissimilation of Glucose by Heterolactic Bacteria

Kawai, Kazuhiro; Yashima, Shigetaka; Okami, Yoshirō; Sasaki, Yuji

doi:10.2323/jgam.17.51

Cited by 10 publications

(6 citation statements)

References 5 publications

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“…Although activities of NADH2 oxidase and NADPH2 oxidase were detected in the organism (9), it was previously reported that lactic acid formation by this organism was hardly affected by oxygen, while, under aerobic conditions, ethonol production decreased with a corresponding increase in acetate production indicating the existence of some mechanism of specific protection of lactic acid-forming system against the effect of oxygen ( 8 ). It is very interesting that ALDH and ADH of the organism were detected as insoluble enzymes suggesting the possibility of different localization of lactic acid dehydrogenase and glyceraldehyde 3-phosphate dehydrogenase from ALDH, ADH, NADH2 oxidase or NADPH2 oxidase.…”

Section: Discussionmentioning

confidence: 95%

“…mesenteroides IFO 3426 was used throughout this study and procedures for cultivation and preparation of washed cells were the same as previously reported ( 9).…”

Section: Organismmentioning

confidence: 99%

“…NADH2 oxidase, NADPH2 oxidase, NADH2 peroxidase or NADPH2 peroxidase activity was assayed by the method reported previously (9). Activities of lactate dehydrogenase and acetate kinase were determined according to the methods of DENNIS and KAPLAN (10) and ROSE et al (I1), respectively.…”

Section: Organismmentioning

confidence: 99%

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Aerobic Dissimilation of Glucose by Heterolactic Bacteria

KAZAHAYA

Kawai

Yashima

et al. 1972

J. Gen. Appl. Microbiol.

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Section: Discussionmentioning

confidence: 95%

“…mesenteroides IFO 3426 was used throughout this study and procedures for cultivation and preparation of washed cells were the same as previously reported ( 9).…”

Section: Organismmentioning

confidence: 99%

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Aerobic Dissimilation of Glucose by Heterolactic Bacteria

KAZAHAYA

Kawai

Yashima

et al. 1972

J. Gen. Appl. Microbiol.

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“…It is, however, inconvenient that pre-aeration of washed cells of L. buchneri without addition of glucose did not induce anaerobic dissimilation of glucose, whereas they possess both NADH2 and NADPH2 oxidases similar to those from Leuconostoc mesenteroides ( 22 ). Moreover, the most of intracellular pyridine nucleotide coenzymes were found to be in oxidized form in the washed cells just before their use in the experiments (unpublished results).…”

Section: Discussionmentioning

confidence: 98%

Induction of Anaerobic Glucose Dissimilation of Lactobacillus Buchneri by Acetate

Kakimoto

Kawai

Yashima

et al. 1972

J. Gen. Appl. Microbiol.

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Aeration during the early period of glucose dissimilation by resting cells of Lactobacillus buchneri IFO 3961 was found to induce succeeding anaerobic dissimilation of glucose, and it was confirmed that acetate, one of products in aerobic glucose dissimilation, is a compound which has the ability to induce anaerobic glucose dissimilation of this organism.The metabolic pathway of heterolactic fermentation seemed to be well established by several investigators, suggesting that glucose dissimilation by resting cells of heterolactic bacteria may operate under anaerobic conditions (1-15).As previously reported, however, we found that glucose dissimilation by resting cells of Lactobacillus brevis or L. buchneri was strongly repressed under static conditions, whereas they dissimilate glucose under aerobic conditions producing lactate, acetate and ethanol (16). From the metabolic pathway proposed for heterolactic fermentation, the explanation for these phenomena was considered to be impossible, and it was assumed that oxygen may affect the ability of these organisms for glucose dissimilation.During the course of studies on the effect of oxygen on the dissimilative activity of L, buchneri, we found a remarkable effect of oxygen on the ability of anaerobic glucose dissimilation of this organism, and its details are described in this report. MATERIALS AND METHODS Organisms.Lactobacillus buchneri IFO 3961 was used in the experiment on the dissimilation of glucose and Escherichia coli IAM 1264 (K-12) for the preparation of acetokinase.Media and culture conditions. The medium for the stock culture of L. buchneri contained (in percent), glucose, 0.5; polypeptone, 1.0; meat extract, 0.5; sodium chloride, 0.5; yeast extract, 1.0; and agar powder, 1.5; (pH 7.0). The organism was maintained as a stab culture in the medium described 1

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“…Moreover, NADH2 oxidase and NADPH2 oxidase were partially purified from this organism, and basic characteristics of these oxidases were previously reported (9).…”

mentioning

confidence: 84%