Aerobic exercise improves adipogenesis in diet-induced obese mice via the lncSRA/p38/JNK/PPARγ pathway

Wu, Bidong; Jing, Ding; Chen, Anping; Song, Yicun; Xu, Cong; Tian, Fei; Zhao, Jinfeng

doi:10.1016/j.nutres.2022.04.004

Cited by 9 publications

(5 citation statements)

References 36 publications

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“…Moderate-intensity exercise reduced the Lee index, while low-intensity exercise did not impede its increase. This supports previous studies showing moderate-intensity exercise can reduce the Lee index in obese mice (Fauzi et al, 2020;Mostarda et al, 2012).The similar results also showed that moderate-intensity exercise for 8 weeks distinctly lowered the Lee index of dietinduced obese mice compared to the untreated group (Wu et al, 2022). The Lee index can be used to measure obesity as it correlates with white adipose tissue deposits (Malafaia et al, 2013).…”

Section: Discussionsupporting

confidence: 89%

The Effect of Exercise Intensity on Anthropometric Parameters and Renal Damage in High Fructose- Induced Mice

Sari,

Ramadhan,

Agustin

et al. 2023

Retos

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Excessive fructose intake disrupts carbohydrate and lipid metabolism in the kidney, resulting in kidney injury. Exercise has proven to improve the renal fatty acid metabolism, but the effect of various exercise intensities in preventing renal disorders is still unknown. The purpose of this study is to analyze the effect of various exercise intensities on anthropometric parameters and renal damage in high fructose-induced mice. The subjects were thirty-six male mice (20–30 g), aged 8 weeks were obtained and randomly assigned into 4 groups: HFr-Sed (sedentary), HFr-Ex1 (low-intensity exercise), HFr-Ex2 (moderate-intensity exercise), and HFr-Ex3 (high-intensity exercise). They were fed standard chow and high fructose solution (30%), per-oral, ad libitum for 8 weeks. The exercised groups underwent swimming, with 80% maximum duration/session, 3x/week, for 8 weeks. The result showed that there were significant differences in body weight (p < 0.001), body length (p = 0.001), Lee index (p = 0.020), Body Mass Index (BMI) (p = 0.004), and serum creatinine (SCr) level (p < 0.001). However, the glomerulosclerosis index and interstitial fibrosis degree were not significantly different in all groups. It can be concluded that various intensities of exercise affect the body composition and SCr level, especially moderate-intensity exercise, but do not impact the improvement of the histological kidney in high fructose-induced mice. Keyword: Exercise, healthy lifestyle, obesity, renal histology.

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Section: Discussionsupporting

confidence: 89%

The Effect of Exercise Intensity on Anthropometric Parameters and Renal Damage in High Fructose- Induced Mice

Sari,

Ramadhan,

Agustin

et al. 2023

Retos

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“…The p38 pathway mainly controls the activation of BAT and browning of WAT by inducing UCP1 transcription through the activation of cAMP response element-binding protein (CREB), ATF2, and PGC1α ( 54 ). Chronic HFD feeding inhibits activation of the p38/MAPK signaling pathway, whereas aerobic exercise training significantly improves p38 phosphorylation levels and lipid metabolism in obese mice ( 55 ). Similar to this result, our findings also demonstrated restoration of the P-p38/p38MAPK ratio against HFD-mediated loss in PVAT.…”

Section: Discussionmentioning

confidence: 99%

Exercise attenuates high-fat diet-induced PVAT dysfunction through improved inflammatory response and BMP4-regulated adipose tissue browning

Liu,

Jiang,

et al. 2024

Front. Nutr.

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BackgroundPerivascular adipose tissue (PVAT) dysfunction impairs vascular homeostasis. Impaired inflammation and bone morphogenetic protein-4 (BMP4) signaling are involved in thoracic PVAT dysfunction by regulating adipokine secretion and adipocyte phenotype transformation. We investigated whether aerobic exercise training could ameliorate high-fat diet (HFD)-induced PVAT dysfunction via improved inflammatory response and BMP4-mediated signaling pathways.MethodsSprague-Dawley rats (n = 24) were divided into three groups, namely control, high-fat diet (HFD), and HFD plus exercise (HEx). After a 6-week intervention, PVAT functional efficiency and changes in inflammatory biomarkers (circulating concentrations in blood and mRNA expressions in thoracic PVAT) were assessed.ResultsChronic HFD feeding caused obesity and dyslipidemia in rats. HFD decreased the relaxation response of PVAT-containing vascular rings and impaired PVAT-regulated vasodilatation. However, exercise training effectively reversed these diet-induced pathological changes to PVAT. This was accompanied by significantly (p < 0.05) restoring the morphological structure and the decreased lipid droplet size in PVAT. Furthermore, HFD-induced impaired inflammatory response (both in circulation and PVAT) was notably ameliorated by exercise training (p < 0.05). Specifically, exercise training substantially reversed HFD-induced WAT-like characteristics to BAT-like characteristics as evidenced by increased UCP1 and decreased FABP4 protein levels in PVAT against HFD. Exercise training promoted transcriptional activation of BMP4 and associated signaling molecules (p38/MAPK, ATF2, PGC1α, and Smad5) that are involved in browning of adipose tissue. In conjunction with gene expressions, exercise training increased BMP4 protein content and activated downstream cascades, represented by upregulated p38/MAPK and PGC1α proteins in PVAT.ConclusionRegular exercise training can reverse HFD-induced obesity, dyslipidemia, and thoracic PVAT dysfunction in rats. The browning of adipose tissue through exercise appears to be modulated through improved inflammatory response and/or BMP4-mediated signaling cascades in obese rats.

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“…Pathways related to extracellular matrix remodeling and circadian rhythm were found to be regulated by MSCs in response to a high-fat diet and exercise [37]. Numerous studies are currently investigating the regulatory functions of lncRNAs in obesity development, adipogenesis, and the impact of physical activity on lncRNA expression and regulation [38][39][40][41]. Additionally, Martinez et al reported the existence of thousands of unannotated small open reading frames (smORFs) within protein-coding genes and lncRNAs in mouse adipocytes.…”

Section: Discussionmentioning

confidence: 99%

Unveiling Polysomal Long Non-Coding RNA Expression on the First Day of Adipogenesis and Osteogenesis in Human Adipose-Derived Stem Cells

Bonilauri,

Ribeiro,

Spangenberg

et al. 2024

IJMS

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Understanding the intricate molecular mechanisms governing the fate of human adipose-derived stem cells (hASCs) is essential for elucidating the delicate balance between adipogenic and osteogenic differentiation in both healthy and pathological conditions. Long non-coding RNAs (lncRNAs) have emerged as key regulators involved in lineage commitment and differentiation of stem cells, operating at various levels of gene regulation, including transcriptional, post-transcriptional, and post-translational processes. To gain deeper insights into the role of lncRNAs’ in hASCs’ differentiation, we conducted a comprehensive analysis of the lncRNA transcriptome (RNA-seq) and translatome (polysomal-RNA-seq) during a 24 h period of adipogenesis and osteogenesis. Our findings revealed distinct expression patterns between the transcriptome and translatome during both differentiation processes, highlighting 90 lncRNAs that are exclusively regulated in the polysomal fraction. These findings underscore the significance of investigating lncRNAs associated with ribosomes, considering their unique expression patterns and potential mechanisms of action, such as translational regulation and potential coding capacity for microproteins. Additionally, we identified specific lncRNA gene expression programs associated with adipogenesis and osteogenesis during the early stages of cell differentiation. By shedding light on the expression and potential functions of these polysome-associated lncRNAs, we aim to deepen our understanding of their involvement in the regulation of adipogenic and osteogenic differentiation, ultimately paving the way for novel therapeutic strategies and insights into regenerative medicine.

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Aerobic exercise improves adipogenesis in diet-induced obese mice via the lncSRA/p38/JNK/PPARγ pathway

Cited by 9 publications

References 36 publications

The Effect of Exercise Intensity on Anthropometric Parameters and Renal Damage in High Fructose- Induced Mice

The Effect of Exercise Intensity on Anthropometric Parameters and Renal Damage in High Fructose- Induced Mice

Exercise attenuates high-fat diet-induced PVAT dysfunction through improved inflammatory response and BMP4-regulated adipose tissue browning

Unveiling Polysomal Long Non-Coding RNA Expression on the First Day of Adipogenesis and Osteogenesis in Human Adipose-Derived Stem Cells

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