Aerobic methanotrophs from the coastal thermal springs of Lake Baikal

Zelenkina, T. S.; Eshinimayev, B. Ts.; Дагурова, О. П.; Suzina, N. E.; Namsarayev, B. B.; Trotsenko, Yu. A.

doi:10.1134/s0026261709040134

Cited by 11 publications

(4 citation statements)

References 11 publications

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“…Methylocystis strain Se48 had a reported optimal growth temperature of 37°C and a maximal growth temperature of 53°C (Tsyrenzhapova et al, 2007). Using fluorescence in situ hybridization (FISH), Zelenkina et al (2009) reported that members of the genus Methylocystis constituted the majority (up to 99%) of the total alphaproteobacterial methanotrophs in enrichment cultures from the coastal thermal springs of Lake Baikal at 45 and 51°C. Members of the genera Methylosinus and Methylocapsa were found in very low abundance (< 1%), and no Methylocapsa pmoA gene sequences could be amplified from the enrichment cultures.…”

Section: Discussionmentioning

confidence: 99%

Methanotrophic bacteria in warm geothermal spring sediments identified using stable-isotope probing

Sharp

Martínez-Lorenzo

Brady

et al. 2014

FEMS Microbiol Ecol

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We investigated methanotrophic bacteria in sediments of several warm geothermal springs ranging in temperature from 22 to 45 °C. Methane oxidation was measured at potential rates up to 141 μmol CH4 d(-1) g(-1) sediment. Active methanotrophs were identified using (13) CH4 stable-isotope probing (SIP) incubations performed at close to in situ temperatures for each site. Quantitative (q) PCR of pmoA genes identified the position of the heavy ((13) C-labelled) DNA fractions in density gradients, and 16S rRNA gene pyrotag sequencing of the heavy fractions was performed to identify the active methanotrophs. Methanotroph communities identified in heavy fractions of all samples were predominated by species similar (≥ 95% 16S rRNA gene identities) to previously characterized Gammaproteobacteria and Alphaproteobacteria methanotrophs. Among the five hottest samples (45 °C), members of the Gammaproteobacteria genus Methylocaldum dominated in two cases, while three others were dominated by an OTU closely related (96.8% similarity) to the Alphaproteobacteria genus Methylocapsa. These results suggest that diverse methanotroph groups are adapted to warm environments, including the Methylocapsa-Methylocella-Methyloferula group, which has previously only been detected in cooler sites.

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Section: Discussionmentioning

confidence: 99%

Methanotrophic bacteria in warm geothermal spring sediments identified using stable-isotope probing

Sharp

Martínez-Lorenzo

Brady

et al. 2014

FEMS Microbiol Ecol

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“…Currently, all cultivated Methylocystis strains have reported maximum growth temperatures of ≤53°C (Tsypenzhapova et al, 2007). Molecular-based surveys have detected pmoA sequences similar to Methylocystis and Methylocapsa in a 51°C hot spring (Zelenkina et al, 2009) and in geothermal sediments up to 76°C (Sharp et al, 2014b), while Kizilova et al (2014) demonstrated 14 CH 4 bio-assimilation in hot spring samples containing Alphaproteobacteria OTUs incubated at 75°C (Kizilova et al, 2014), further supporting the possibility of thermophilic alphaproteobacterial methanotrophs within these microcosms.…”

Section: Microcosms Support Both Aerobic and Anaerobic Methanotrophsmentioning

confidence: 82%

Thermophilic methane oxidation is widespread in Aotearoa-New Zealand geothermal fields

Houghton,

Carere,

Stott

et al. 2023

Front. Microbiol.

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Geothermal areas represent substantial point sources for greenhouse gas emissions such as methane. While it is known that methanotrophic microorganisms act as a biofilter, decreasing the efflux of methane in most soils to the atmosphere, the diversity and the extent to which methane is consumed by thermophilic microorganisms in geothermal ecosystems has not been widely explored. To determine the extent of biologically mediated methane oxidation at elevated temperatures, we set up 57 microcosms using soils from 14 Aotearoa-New Zealand geothermal fields and show that moderately thermophilic (>40°C) and thermophilic (>60°C) methane oxidation is common across the region. Methane oxidation was detected in 54% (n = 31) of the geothermal soil microcosms tested at temperatures up to 75°C (pH 1.5–8.1), with oxidation rates ranging from 0.5 to 17.4 μmol g−1 d−1 wet weight. The abundance of known aerobic methanotrophs (up to 60.7% Methylacidiphilum and 11.2% Methylothermus) and putative anaerobic methanotrophs (up to 76.7% Bathyarchaeota) provides some explanation for the rapid rates of methane oxidation observed in microcosms. However, not all methane oxidation was attributable to known taxa; in some methane-consuming microcosms we detected methanotroph taxa in conditions outside of their known temperature range for growth, and in other examples, we observed methane oxidation in the absence of known methanotrophs through 16S rRNA gene sequencing. Both of these observations suggest unidentified methane oxidizing microorganisms or undescribed methanotrophic syntrophic associations may also be present. Subsequent enrichment cultures from microcosms yielded communities not predicted by the original diversity studies and showed rates inconsistent with microcosms (≤24.5 μmol d−1), highlighting difficulties in culturing representative thermophilic methanotrophs. Finally, to determine the active methane oxidation processes, we attempted to elucidate metabolic pathways from two enrichment cultures actively oxidizing methane using metatranscriptomics. The most highly expressed genes in both enrichments (methane monooxygenases, methanol dehydrogenases and PqqA precursor peptides) were related to methanotrophs from Methylococcaceae, Methylocystaceae and Methylothermaceae. This is the first example of using metatranscriptomics to investigate methanotrophs from geothermal environments and gives insight into the metabolic pathways involved in thermophilic methanotrophy.

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“…However, Auman et al [27] reported the highest oxidation rate of M to be 346 mol l −1 h −1 at depths between 0 and 0.5 cm in sediments from Lake Washington, USA. Zelenkina et al [28] reported uptake rates between 0.2 and 7.7 nmol cm −1 h −1 M with sediment from Lake Baikal.…”

Section: Comparison Of M and Bt Oxidationmentioning

confidence: 97%

Characterization of methane, benzene and toluene-oxidizing consortia enriched from landfill and riparian wetland soils

Lee¹,

Park²,

Cho³

2010

Journal of Hazardous Materials

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Aerobic methanotrophs from the coastal thermal springs of Lake Baikal

Cited by 11 publications

References 11 publications

Methanotrophic bacteria in warm geothermal spring sediments identified using stable-isotope probing

Methanotrophic bacteria in warm geothermal spring sediments identified using stable-isotope probing

Thermophilic methane oxidation is widespread in Aotearoa-New Zealand geothermal fields

Characterization of methane, benzene and toluene-oxidizing consortia enriched from landfill and riparian wetland soils

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