Aerobiosis–anaerobiosis transition has a significant impact on organic acid production by Corynebacterium glutamicum

Kaboré, Abdoul-Karim; Olmos, Éric; Fick, Michel; Blanchard, Fabrice; Guédon, Emmanuel; Delaunay, Stéphane

doi:10.1016/j.procbio.2016.10.007

Cited by 7 publications

(16 citation statements)

References 36 publications

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“…Additionally, different strategies of transition from the aerobic to anaerobic conditions have been studied. Compared to an instantaneous transition from aerobiosis to anaerobiosis, applying an optimized transition strategy led to an increase in the succinate concentration up to 640% . This positive impact of a micro‐aerobic environment on the synthesis of succinate was also observed in E. coli .…”

Section: Introductionmentioning

confidence: 83%

“…In this study, the best transition strategy proposed by Kaboré et al. was applied: the dissolved oxygen level was switched from 50 to 20% then to 10% of air saturation. The DOC was maintained for at least 2 h for each level.…”

Section: Methodsmentioning

confidence: 99%

“…The resultant strain was able to produce 134 g L −1 succinate with a yield of 1.67 mol mol −1 glucose using the two‐step process with an anaerobic fed batch phase. Alternatively, an one‐step process with successive aerobic transition and anaerobic phases in the same bioreactor was set up . These last authors demonstrated that the transition from aerobic to anaerobic conditions, defined as micro‐aerobic conditions, had a strong influence on organic acid production.…”

Section: Introductionmentioning

confidence: 99%

“…According to Kaboré et al. , micro‐aerobic conditions consist in a constantly limiting oxygen transfer rate but with non‐zero dissolved oxygen concentrations. Similarly, Byun et al.…”

Section: Introductionmentioning

confidence: 99%

“…It was also shown, by determination of the volumetric mass transfer coefficient ( k L a ) during cultures in shake flask and using a mutant strain of C. glutamicum deleted for lactate dehydrogenase (LDH), that a critical oxygenation level had to be defined according to the produced organic acid. The optimal transfer condition corresponded to k L a values between 10 and 19 h −1 for succinate production and, 19 and 33 h −1 for acetate production . Additionally, different strategies of transition from the aerobic to anaerobic conditions have been studied.…”

Section: Introductionmentioning

confidence: 99%

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Corynebacterium glutamicum, a natural overproducer of succinic acid?

Briki

Kaboré

Olmos

et al. 2020

Engineering in Life Sciences

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Corynebacterium glutamicum is well known as an important industrial amino acid producer. For a few years, its ability to produce organic acids, under micro-aerobic or anaerobic conditions was demonstrated. This study is focused on the identification of the culture parameters influencing the organic acids production and, in particular, the succinate production, by this bacterium. Corynebacterium glutamicum 2262, used throughout this study, was a wild-type strain, which was not genetically designed for the production of succinate. The oxygenation level and the residual glucose concentration appeared as two critical parameters for the organic acids production. The maximal succinate concentration (4.9 g L −1 ) corresponded to the lower k L a value of 5 h −1 .Above 5 h −1 , a transient accumulation of the succinate was observed. Interestingly, the stop in the succinate production was concomitant with a lower threshold glucose concentration of 9 g L −1 . Taking into account this threshold, a fed-batch culture was performed to optimize the succinate production with C. glutamicum 2262. The results showed that this wild-type strain was able to produce 93.6 g L −1 of succinate, which is one of the highest concentration reported in the literature. K E Y W O R D SCorynebacterium glutamicum, oxygen transfer, residual glucose concentration, succinate Abbreviations: DOC, dissolved oxygen concentration; k L a, volumetric mass transfer coefficient; LDH, lactate dehydrogenase.This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Section: Introductionmentioning

confidence: 83%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…According to Kaboré et al. , micro‐aerobic conditions consist in a constantly limiting oxygen transfer rate but with non‐zero dissolved oxygen concentrations. Similarly, Byun et al.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Corynebacterium glutamicum, a natural overproducer of succinic acid?

Briki

Kaboré

Olmos

et al. 2020

Engineering in Life Sciences

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From Microbial Succinic Acid Production to Polybutylene Bio‐Succinate Synthesis

Tenhaef

Hermann

Müller

et al. 2023

Chemie Ingenieur Technik

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A simplified and scalable one‐pot process for the anaerobic production of succinic acid using a metabolically engineered Corynebacterium glutamicum strain is demonstrated. With targeted bioprocess optimization, succinic acid titer of 78 g L−1 and yield of 1.41 molSAmolGLC−1 were achieved. Succinic acid was recovered from the neutral fermentation broth by electrochemically induced crystallization and applied for polybutylene bio‐succinate synthesis using a biocompatible zinc catalyst. Except for a slight color change, the final biopolymer was comparable to the polymer from commercial precursors.

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Physiological characteristics of Corynebacterium glutamicum as a cell factory under anaerobic conditions

Tsuge

Yamaguchi

2021

Appl Microbiol Biotechnol

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Aerobiosis–anaerobiosis transition has a significant impact on organic acid production by Corynebacterium glutamicum

Cited by 7 publications

References 36 publications

Corynebacterium glutamicum, a natural overproducer of succinic acid?

Corynebacterium glutamicum, a natural overproducer of succinic acid?

From Microbial Succinic Acid Production to Polybutylene Bio‐Succinate Synthesis

Physiological characteristics of Corynebacterium glutamicum as a cell factory under anaerobic conditions

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