Aeromonas–Acanthamoeba interaction and early shift to a viable but nonculturable state of Aeromonas by Acanthamoeba

Rahman, Mahbubur; Abd, Hadi; Römling, Ute; Sandström, Gunnar; Möllby, R.

doi:10.1111/j.1365-2672.2007.03687.x

Cited by 24 publications

(21 citation statements)

References 47 publications

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“…aeruginosa and A. hydrophila where the cell counts exceeded 5 log cycles at time 0h with highly significant differences versus other bacterial strains (P <0.001 for both) and increased significantly in number to reach >8 and >7 log cycle at time 24h of co-incubation, respectively in comparison to other isolates (p <0.001 for both). The high multiplication rate of A. hydrophila inside FLA in our study has been demonstrated before [22,23]. Ps.…”

Section: Co-culture Of a Spelaea And Bacteriasupporting

confidence: 70%

Allovahlkampfia spelaea is a Potential Environmental Host for Pathogenic Bacteria

Mohamed¹,

Huseein²,

Farrag³

et al. 2016

J Bacteriol Parasitol

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Allovahlkampfia spelaea was identified for the first time in 2009. As a free living amoeba, it has been suggested to be a protective host for some bacterial pathogens against harsh environmental conditions and can transmit them to vulnerable hosts. We aimed in this study to test the interactions between Allovahlkampfia spelaea and some waterborne and foodborne bacteria and unravel if the tested bacteria can survive and multiply inside amoeba. We used a keratitis isolate of Allovahlkampfia spelaea grown in PYG medium containing proteose peptone, yeast extracts, and glucose. We examined amoeba interactions with Methicillin resistant Staphylococcus aureus, Escherichia coli 1, Klebsiella pneumoniae, Enterobacter aerogenes, Citrobacter cloaca, Proteus mirabilis, Raoultella terrigena, Raoultella ornitholytica, Aeromonas hydrophila and Pseudomonas aeruginosa using the co-culture assays. Amoebal survival rate with different bacterial strains were determined. With the exception of Proteus mirabilis that showed decreased survival rates inside amoebal cells, other bacterial isolates could survive and multiply inside Allovahlkampfia spelaea that was associated with decreased survival rates of the amoeba. Particularly, Pseudomonas aeruginosa, Aeromonas hydrophila and MRSA exhibited significantly increased multiplication rates inside amoeba. Our study demonstrated that Allovahlkampfia spelaea may act as a replicative host for pathogenic bacteria with environmental and clinical implications.

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Section: Co-culture Of a Spelaea And Bacteriasupporting

confidence: 70%

Allovahlkampfia spelaea is a Potential Environmental Host for Pathogenic Bacteria

Mohamed¹,

Huseein²,

Farrag³

et al. 2016

J Bacteriol Parasitol

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“…The ability of P. aeruginosa to resist amoeba predation has been described by others (Wang & Ahearn, 1997), and it has been demonstrated recently that this lysis is related to necrosis and apoptosis, and mediated by the effect of type III secretion system effector proteins (Abd et al, 2008). The pathogenicity of P. aeruginosa in humans has been demonstrated to be associated with resistance to its lytic effect on amoebae (Fenner et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

“…Survival of Aeromonas salmonicida has been observed in protozoa of the species Tetrahymena pyriformis (King & Shotts, 1988), and recently it has been demonstrated that free-living amoeba might play a role as a reservoir of Aeromonas spp. (Rahman et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Amoeba co-culture of soil specimens recovered 33 different bacteria, including four new species and Streptococcus pneumoniae

et al. 2009

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Amoeba-resistant bacteria (ARB), such as Legionella spp., are currently regarded as potential human pathogens that live in the natural environment, and thus their habitat is regarded as a reservoir of human pathogens. To detect ARB in human and environmental samples, co-culture with amoebae has been demonstrated to be an efficient tool. However, to date, only water samples from cooling towers and hospital water supplies have been investigated as possible reservoirs of ARB using this procedure. In the present study, we studied the ARB population of 11 diverse soil and sand sources in proximity to human environments; these sources included the university, the station, hospitals, the square, parks and public beaches in the city of Marseilles, France. As a result, a total of 33 different species of ARB were identified. The ability to grow within and/or lyse amoebae was demonstrated, for what is believed to be the first time, for several species; moreover, 20 of the isolates (61 %), including Streptococcus pneumoniae, have been described as human pathogens. However, Legionella spp. were not isolated. Four isolates are likely to be the members of new or uncharacterized genera or species, and their capability to be human pathogens needs to be determined. This preliminary work demonstrates that soils and sands in the vicinity of humans are reservoirs of human pathogenic ARB.

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“…(235,242), has the capacity to multiply in the amoeba, and has cytopathic effects. The species Aeromonas hydrophila was found to interact actively with A. castellanii and to be able to survive within cysts (245,246).…”

Section: Fig 3 Amoeba Culturing Methods For Recovering Amoeba-resistimentioning

confidence: 99%

Current and Past Strategies for Bacterial Culture in Clinical Microbiology

et al. 2015

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SUMMARY A pure bacterial culture remains essential for the study of its virulence, its antibiotic susceptibility, and its genome sequence in order to facilitate the understanding and treatment of caused diseases. The first culture conditions empirically varied incubation time, nutrients, atmosphere, and temperature; culture was then gradually abandoned in favor of molecular methods. The rebirth of culture in clinical microbiology was prompted by microbiologists specializing in intracellular bacteria. The shell vial procedure allowed the culture of new species of Rickettsia . The design of axenic media for growing fastidious bacteria such as Tropheryma whipplei and Coxiella burnetii and the ability of amoebal coculture to discover new bacteria constituted major advances. Strong efforts associating optimized culture media, detection methods, and a microaerophilic atmosphere allowed a dramatic decrease of the time of Mycobacterium tuberculosis culture. The use of a new versatile medium allowed an extension of the repertoire of archaea. Finally, to optimize the culture of anaerobes in routine bacteriology laboratories, the addition of antioxidants in culture media under an aerobic atmosphere allowed the growth of strictly anaerobic species. Nevertheless, among usual bacterial pathogens, the development of axenic media for the culture of Treponema pallidum or Mycobacterium leprae remains an important challenge that the patience and innovations of cultivators will enable them to overcome.

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Aeromonas–Acanthamoeba interaction and early shift to a viable but nonculturable state of Aeromonas by Acanthamoeba

Cited by 24 publications

References 47 publications

Allovahlkampfia spelaea is a Potential Environmental Host for Pathogenic Bacteria

Allovahlkampfia spelaea is a Potential Environmental Host for Pathogenic Bacteria

Amoeba co-culture of soil specimens recovered 33 different bacteria, including four new species and Streptococcus pneumoniae

Current and Past Strategies for Bacterial Culture in Clinical Microbiology

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