Aeromonas hydrophila Induces Skin Disturbance through Mucosal Microbiota Dysbiosis in Striped Catfish (
            <i>Pangasianodon hypophthalmus</i>
            )

Chen, Li-Hsuan; Lin, Chia-Hsuan; Siao, Ru-Fang; Wang, Liangchun

doi:10.1128/msphere.00194-22

Cited by 9 publications

(3 citation statements)

References 59 publications

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“…Aeromonas hydrophila , e.g. is ubiquitous within freshwater environments and is associated with diseases including bacterial haemorrhagic septicaemia and epizootic ulcerative syndrome (Lategan et al 2004 , Chen et al 2022 ). Flavobacterium psychrophilum is the primary agent of bacterial cold-water disease and rainbow trout fry syndrome, which is one of the main sources of economic loss of the salmonid industry (Duchaud et al 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Influence of host phylogeny and water physicochemistry on microbial assemblages of the fish skin microbiome

Bell,

McMurtrie,

Bolaños

et al. 2024

FEMS Microbiology Ecology

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The skin of fish contains a diverse microbiota that has symbiotic functions with the host, facilitating pathogen exclusion, immune system priming and nutrient degradation. The composition of fish skin microbiomes varies across species and in response to a variety of stressors, however, there has been no systematic analysis across these studies to evaluate how these factors shape fish skin microbiomes. Here, we examined 1922 fish skin microbiomes from 36 studies that included 98 species and nine rearing conditions to investigate associations between fish skin microbiome, fish species, and water physiochemical factors. Proteobacteria, particularly the class Gammaproteobacteria, were present in all marine and freshwater fish skin microbiomes. Acinetobacter, Aeromonas, Ralstonia, Sphingomonas and Flavobacterium were the most abundant genera within freshwater fish skin microbiomes, and Alteromonas, Photobacterium, Pseudoalteromonas, Psychrobacter and Vibrio were the most abundant in saltwater fish. Our results show that different culturing (rearing) environments have a small but significant effect on the skin bacterial community compositions. Water temperature, pH, dissolved oxygen concentration and salinity significantly correlated with differences in beta-diversity but not necessarily alpha-diversity. To improve study comparability on fish skin microbiomes, we provide recommendations for approaches to the analyses of sequencing data and improve study reproducibility.

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Section: Discussionmentioning

confidence: 99%

Influence of host phylogeny and water physicochemistry on microbial assemblages of the fish skin microbiome

Bell,

McMurtrie,

Bolaños

et al. 2024

FEMS Microbiology Ecology

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“…Generally, opportunistic pathogens proliferate when the host is under stress ( 44 ). A significant increase in opportunistic bacteria has been reported in the skin of diseased fish ( 45 , 46 ). Proliferation of Klebsiella in the feces of infected fish may also be associated with stress in ayu as indicated by the increase in cortisol concentrations.…”

Section: Discussionmentioning

confidence: 99%

Fecal metagenomic and metabolomic analyses reveal non-invasive biomarkers of Flavobacterium psychrophilum infection in ayu ( Plecoglossus altivelis )

Takeuchi,

Fujiwara-Nagata,

Kuroda

et al. 2024

mSphere

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With the rapid growth of inland aquaculture worldwide, side effects such as the discharge of nutrients and antibiotics pose a threat to the global environments. A sustainable future for aquaculture requires an effective management system, including the early detection of disease through the monitoring of specific biomarkers in aquaculture tanks. To this end, we investigated whether fish feces in aquaculture tanks could be used for non-invasive health monitoring using ayu ( Plecoglossus altivelis ) infected with Flavobacterium psychrophilum , which causes bacterial cold-water disease worldwide. Feces that were subsequently produced in the tanks were used for metagenomic and metabolomic analyses. The relative abundances of the genera Cypionkella (0.6% ± 1.0%, 0.1% ± 0.2%), Klebsiella (11.2% ± 10.0%, 6.2% ± 5.9%), and F. psychrophilum (0.5% ± 1.0%, 0.0% ± 0.0%) were significantly higher in the feces of the infection challenge test tanks than in those of the control tanks. The abundances of cortisol, glucose, and acetate in the feces of the infection challenge test tanks were 2.4, 2.4, and 1.3 times higher, respectively, than those of the control tanks. Metagenome analysis suggested that acetate was produced by microbes such as Cypionkella . The abundances of indicated microbes or metabolites increased after day 4 of infection at the earliest, and were thus considered possible biomarkers. Our results suggest that feces produced in aquaculture tanks can potentially be used for non-invasive and holistic monitoring of fish diseases in aquaculture systems. IMPORTANCE The aquaculture industry is rapidly growing, yet sustainability remains a challenge. One crucial task is to reduce losses due to diseases. Monitoring fish health and detecting diseases early are key to establishing sustainable aquaculture. Using metagenomic and metabolomic analyses, we found that feces of ayu infected with Flavobacterium psychrophilum contain various specific biomarkers that increased 4 days post-challenge, at the earliest. Our findings are the first step in establishing a novel, non-invasive, and holistic monitoring method for fish diseases in aquaculture systems, especially in ayu, which is an important freshwater fish species in Asia, promoting a sustainable future.

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“…on the skin samples (Reid et al 2017). A recent study on striped catfish (Pangasianodon hypophthalmus) challenged with Aeromonas hydrophila similarly showed that pathogen concentration stimulates the skin immune response by altering the mucosal microbiota and increasing the prevalence of opportunistic pathogens (Chen et al 2022). Li et al (2017b) reported that during "red-operculum" disease in crucian carp (Carassius auratus), genera belonging to Aeromonas, Vibrio, and Shewanella were dominant while in healthy fish genera from Cetobacterium, Cyanobacterium and Clostridiaceae were more abundant.…”

Section: Diseases/infectionsmentioning

confidence: 99%

Tilapia aquaculture, emerging diseases, and the roles of the skin microbiomes in health and disease

et al. 2023

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Aquaculture is playing an increasingly important role in global food security, especially for low-income and food-deficit countries. The majority of aquaculture production occurs in freshwater earthen ponds and tilapia has quickly become one of the most widely adopted culture species in these systems. Tilapia are now farmed in over 140 countries facilitated by their ease of production, adaptability to a wide range of environmental conditions, fast growth, and high nutritional value. Typically, tilapia have been considered a hardy, disease resilient species; however, the disease is increasing with subsequent threats to the industry as their production is intensified. In this review, we discuss tilapia production, with a focus on Bangladesh as one of the top producing countries, and highlight the problems associated with disease and treatment approaches for them, including the misuse of antimicrobials. We address a key missing component in understanding health and disease processes for sustainable production in aquaculture, specifically the role played by the microbiome. Here we examine the importance of the microbiome in supporting health, focused on the symbiotic microbial community of the fish skin mucosal surface, the abiotic and biotic factors that influence the microbiome, and the shifts that are associated with diseased states. We also identify conserved taxa of skin microbiomes that may be used as indicators of health status for tilapia offering new opportunities to mitigate and manage the disease and optimize environmental growing conditions and farming practices.

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Aeromonas hydrophila Induces Skin Disturbance through Mucosal Microbiota Dysbiosis in Striped Catfish ( Pangasianodon hypophthalmus )

Abstract: The fish skin mucosal microbiota is essential in modulating the host response to the presence of pathogens. Our study provides a platform to study both the correlation and causation of the interactions among the pathogen, fish skin, and the skin mucosal microbiota.

Cited by 9 publications

References 59 publications

Influence of host phylogeny and water physicochemistry on microbial assemblages of the fish skin microbiome

Influence of host phylogeny and water physicochemistry on microbial assemblages of the fish skin microbiome

Fecal metagenomic and metabolomic analyses reveal non-invasive biomarkers of Flavobacterium psychrophilum infection in ayu ( Plecoglossus altivelis )

Tilapia aquaculture, emerging diseases, and the roles of the skin microbiomes in health and disease

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