Aeromonas species exhibit aggregative adherence to HEp-2 cells

Neves, M S; Nunes, Marly Paiva; Milhomem, Arlete Moreira

doi:10.1128/jcm.32.4.1130-1131.1994

Cited by 20 publications

(15 citation statements)

References 9 publications

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“…hydrophila and Aer. veronii biovar sobria (Neves et al 1994). The LogA and LA patterns found in our study have not been previously reported for Aeromonas.…”

Section: Discussioncontrasting

confidence: 55%

“…hydrophila isolates from clinical (Guimarães et al 2002) and seafood (Scoglio et al 2001) sources, suggesting that this adhesion pattern may be common to this species worldwide. Similarly, the AggA pattern has also been reported in both clinical (Neves et al 1994) and seafood isolates (Scoglio et al 2001) of Aer. caviae and in clinical isolates of Aer.…”

Section: Discussionmentioning

confidence: 52%

“…hydrophila has the lowest adhesive ability among the three dominant Aeromonas species. Both HEp-2 and Caco-2 cells have been used to model Aeromonas adhesion to the gastrointestinal epithelium in a number of studies (Grey and Kirov 1993;Kirov and Hayward 1993;Neves et al 1994;Sechi et al 2002). The use of these cell lines in the present study enabled us to assess the variability in the adhesive ability of each Aeromonas species.…”

Section: Discussionmentioning

confidence: 99%

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Prevalence of environmental Aeromonas in South East Queensland, Australia: a study of their interactions with human monolayer Caco-2 cells

et al. 2006

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Aims: To investigate the prevalence of Aeromonas in a major waterway in South East Queensland, Australia, and their interactions with a gut epithelial model using Caco-2 cells. Methods and Results: A total of 81 Aeromonas isolates, collected from a major waterway in South East Queensland, Australia, were typed using a metabolic fingerprinting method, and tested for their adhesion to HEp-2 and Caco-2 cells and for cytotoxin production on Vero cells and Caco-2 cells. Aeromonas hydrophila had the highest (43%) and Aeromonas veronii biovar sobria had the lowest (25%) prevalence. Four patterns of adhesion were observed on both HEp-2 and Caco-2 cell lines. Representative isolates having different phenopathotypes (nine strains) together with two clinical isolates were tested for their translocation ability and for the presence of virulence genes associated with pathogenic Escherichia coli. The rate and degree of translocation across Caco-2 monolayers varied among strains and was more pronounced with LogA pattern. Translocation was associated with the adherence of strains to Caco-2 cells microvilli, followed by internalization into Caco-2 cells. Two Aer. veronii biovar sobria strains were positive for the presence of heat-labile toxin genes, with one strain also positive for Shiga-like toxin gene. Conclusions: Pathogenic strains of Aeromonas carrying one or more virulence characteristics are highly prevalent in the waterways studied and are capable of translocating across a human enterocyte cell model. Significance and Impact of the Study: This study indicates that Aeromonas strains carrying one or more virulence properties are prevalent in local waterways and are capable of translocating in a human enterocyte cell culture model. However, their importance in human gastrointestinal disease has yet to be verified under competitive conditions of the gut.

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“…hydrophila and Aer. veronii biovar sobria (Neves et al 1994). The LogA and LA patterns found in our study have not been previously reported for Aeromonas.…”

Section: Discussioncontrasting

confidence: 55%

Section: Discussionmentioning

confidence: 52%

Section: Discussionmentioning

confidence: 99%

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Prevalence of environmental Aeromonas in South East Queensland, Australia: a study of their interactions with human monolayer Caco-2 cells

et al. 2006

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“…The majority (67%) of the A. caviae strains tested presented an aggregative adherence pattern to Caco-2 cells, in accordance with the previous study. Diffuse and aggregative adherence patterns were observed for A. hydrophila as reported by Guimarães et al (2002) and Neves et al (1994) for Caco-2 and to HEp-2 cells, respectively.…”

Section: Discussionsupporting

confidence: 54%

Interactions of clinical and environmental Aeromonas isolates with Caco-2 and HT29 intestinal epithelial cells

Couto

Oliveira

Queiroz

et al. 2007

Lett Appl Microbiol

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Aim: Evaluation of adherence and invasion of Aeromonas spp. to human colon carcinoma cell lines Caco‐2 and HT29 and assessment of cytotoxic activity. Methods and Results: A number of 27 strains of Aeromonas caviae and 23 strains of Aeromonas hydrophila was analysed. All strains were capable to adhere to sub‐confluent monolayers of Caco‐2 and HT29 cell types, presenting aggregative and diffuse adherence patterns cells, respectively. In the cytotoxic assays all strains showed cytopathic and/or cytotoxic activities to Vero cells. The evaluation of the tetrazolium salt (MTT test) reduction capability was carried out in Vero, Caco‐2, and HT29 cells. MTT test showed that Vero cell line was the most sensitive cell type. In the invasion test, 13 strains were analysed on Caco‐2 and HT29 monolayers. Only two (15%) of the 13 strains, A. hydrophila and A. caviae species, both isolated from vegetables were invasive to Caco‐2 cells. No strains were able to invade the HT29 cells. Conclusions: A. hydrophila and A. caviae isolated from human diarrhoeic faeces, vegetables, and water, were able to adhere to and produce cytotoxic/cytopathic effects in intestinal epithelial cell lines. Significance and Impact of the Study: The presence of Aeromonas spp. in food and water samples expressing virulence factors suggest that these sources may act as dissemination vehicles of human pathogen with implication in the public health.

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“…hydrophila can enter into a VBNC state when grown in nutrient-poor conditions. As also referred by other authors (Neves et al 1994;Thornley et al 1996), the tested Aer. hydrophila strain exhibited an aggregative adherence pattern.…”

Section: Discussionsupporting

confidence: 53%

Changes in adhesion ability of Aeromonas hydrophila during long exposure to salt stress conditions

et al. 2012

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Aims Stressful environmental conditions influence both bacterial growth and expression of virulence factors. In the present study, we evaluated the influence of NaCl on Aeromonas hydrophila adhesiveness at two temperatures. This agent is often involved in clinical cases; however, its pathogenic potential is still not fully understood. Methods and Results Bacteria were grown in presence of 1·7%, 3·4%, 6·0% NaCl over a 188 day period and then reinoculated in fresh Nutrient Broth with incubation at 4 and 24°C. Bacterial adhesiveness was tested on Hep‐2 cells, and specimens were processed for light, scanning and transmission electron microscopy. Adhesive capacity decreased over time with an increase in reduction percentages depending on NaCl concentrations. At 1·7% NaCl, the reduction was apparently temporary and adhesiveness rapidly recovered in revitalized bacteria, while 3·4%, 6·0% NaCl seemed to be detrimental. Normal, elongated and filamentous bacteria retained adhesiveness capability, although with reduced expression, while in spherical cells, this property seemed to be lost or dramatically reduced. Conclusions Our study shows that high osmolarity plays a significant role in adhesion inhibition, therefore having possible implications in the pathogenesis of the infections by Aer. hydrophila. Significance and Impact of the Study This study intends to give a contribution to a better understanding of the pathogenic role of this bacterium whose pathogenicity is still under debate.

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Aeromonas species exhibit aggregative adherence to HEp-2 cells

Cited by 20 publications

References 9 publications

Prevalence of environmental Aeromonas in South East Queensland, Australia: a study of their interactions with human monolayer Caco-2 cells

Prevalence of environmental Aeromonas in South East Queensland, Australia: a study of their interactions with human monolayer Caco-2 cells

Interactions of clinical and environmental Aeromonas isolates with Caco-2 and HT29 intestinal epithelial cells

Changes in adhesion ability of Aeromonas hydrophila during long exposure to salt stress conditions

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