Abstract. Colour is one of the primary aesthetic elements of a visualization. It is often used successfully to encode information such as the importance of a particular part of the diagram or the relationship between two parts. Even so, there are few investigations into the human reading of colour coding on diagrams from the scientific community. In this paper we report on an experiment with graph diagrams comparing a black and white composition with two other colour treatments. We drew our subjects from engineering, art, visual design, physical education, tourism, psychology and social science disciplines. We found that colouring the nodes of interest reduced the time taken to find the shortest path between the two nodes for all subjects. Engineers, tourism and social scientists proved significantly faster with artist/designers just below the overall average speed. From this study, we contribute that adding particular colour treatments to diagrams increases legibility. In addition, preliminary work investigating colour treatments and schemes indicates potential for future gains.