Aestuariicella hydrocarbonica gen. nov., sp. nov., an aliphatic hydrocarbon-degrading bacterium isolated from a sea tidal flat

Abstract

Aestuariicella hydrocarbonica gen. nov., sp. nov., an aliphatic hydrocarbon-degrading bacterium isolated from a sea tidal flat A Gram-staining-negative, strictly aerobic bacterial strain, designated SM-6 T , was isolated from a sea tidal flat of the Dangjin bay, South Korea. Strain SM-6 T was able to degrade a broad range of aliphatic hydrocarbons. Cells were catalase-and oxidase-positive and non-motile rods. Growth of strain SM-6 T was observed at 10-37 8C (optimum, 20-25 8C), at pH 5.5-9.0 (optimum, pH 6.5-7.5) and in the presence of 0-10 % (w/v) NaCl (optimum, 2-3 %). The only isoprenoid quinone detected was ubiquinone-8 (Q-8). C 17:1 v8c, C 11:0 3-OH, summed feature 3 (comprising C 16:1 v7c and/or C 16:1 v6c), C 9:0 3-OH and C 10:0 3-OH were observed as the major cellular fatty acids and phosphatidylethanolamine, phosphatidylglycerol and four unidentified lipids were detected as polar lipids. The G+C content of the genomic DNA was 47.5 mol%. Strain SM-6 T was most closely related to Pseudomaricurvus alkylphenolicus KU41G T (95.5 %), Maricurvus nonylphenolicus KU41E T (94.4 %) and Pseudoteredinibacter isoporae SW-11 T (94.3 %), based on 16S rRNA gene sequences, and phylogenetic analyses showed that strain SM-6 T formed a phyletic lineage distinct from the closely related genera. On the basis of phenotypic, chemotaxonomic and molecular features, strain SM-6 T represents a novel genus and species of the order Alteromonadales in the class Gammaproteobacteria, for which name Aestuariicella hydrocarbonica gen. nov., sp. nov. is proposed. The type strain is SM-6 T (5KACC 18121 T 5JCM 30134 T ).

Exilibacterium tricleocarpae gen. nov., sp. nov., a marine bacterium from coralline algae Tricleocarpa sp.

“…S4). These characteristics were similar to those of Pseudoteredinibacter isoporae SW-11 T [4], Pseudomaricurvus alkylphenolicus KU14G T [28], Maricurvus nonylphenolicus KU41E T [29] and Aestuariicella hydrocarbonica SM-6 T [30]. Strain R142 T was non-motile, while Pseudoteredinibacter isoporae SW-11 T and Pseudomaricurvus alkylphenolicus KU14G T were motile.…”

“…S5). It was reported that A. hydrocarbonica SM-6 T has PE and PG as major polar lipids [30]. However, M. nonylphenolicus KU41E T contained DPG as a major polar lipid [35].…”

“…Growth at different pH was tested at 25 °C for 7 days in marine broth 2216 (BD) amended with different buffers (0.5 pH unit intervals, pH 5-8, 0.1 M KH 2 PO 4 /K 2 HPO 4 ; pH 8.5-10, 0.1 M NaHCO 3 /Na 2 CO 3 ; pH 10.5-11, 0.1 M Na 2 CO 3 /NaOH). The optimal growth temperature was determined on marine agar 2216 (BD) plates after 7-30 days of growth at 5,10,15,20,25,30,33,37,40,45 and 50 °C. The ability to form endospores and hydrolysis of starch and Tweens 20, 40, 60 and 80 were tested as described by Dong and Cai [26].…”

Microbial community composition of deep-sea corals from the Red Sea provides insight into functional adaption to a unique environment

“…We further identified Methylibium petroleiphilum 48, Oleibacter sp. 49, Acinetobacter venetianus 50 , Marinobacter bryozoorum 41, Thalassospira xiamenensis 51, and Aestuariicella hydrocarbonica (OTU0009, annotation to NCBI ‘nr’, 99% sequence similarity)52, all of which have been described to possess the potential to degrade crude oil (Supplementary Dataset S1). Considering all oil-degraders that could be assigned to the species level, about 30% of all sequences for Dendrophyllia sp., 5% for E. fistula , and only 0.2% for R. typus were on average comprised by these bacteria.…”

Pseudomaricurvus alcaniphilus sp. nov., a marine bacterium isolated from tidal flat sediment and emended descriptions of the genus Pseudomaricurvus, Pseudomaricurvus alkylphenolicus Iwaki et al. 2014 and Maricurvus nonylphenolicus Iwaki et al. 2012

“…Therefore, the nearly complete 16S rRNA gene sequence (1348 bp) of strain MEBiC06469 T was compared with that of the top 20 closely related strains. Recently, Aestuariicella hydrocarbonica SM-6 T was described and shows 95.1 % 16S rRNA gene sequence similarity (Lo et al , 2015), but was not included in this study. A phylogenetic analysis using the 16S rRNA gene sequence was conducted using mega software version 5.2 (Tamura et al , 2011).…”