Affinity chromatography of porcine pepsin A using quinolin-8-ol as ligand

Protein Binding-Induced Surfactant Aggregation Variation: A New Strategy of Developing Fluorescent Aqueous Sensor for Proteins

“…The detection limits (DLs) of the present sensor system to pepsin and o-egg are determined to be 4.8 nM (0.168 μg mL –1 ) and 18.9 nM (0.837 μg mL –1 ), respectively, according to the 3σ IUPAC criteria. Presently, there are several methods that have been reported to detect these two proteins, such as chromatographic methods, − imprinting hybrid thin films or imprinting polymers, , fluorescent nanoparticles, and voltammetric immunosensor . By comparison, chromatographic analysis exhibits lower sensitivity at milligram levels, − while sensitivity information is not available for imprinting methods. , The fluorescent nanoparticles show competitive sensitivity to pepsin (DL of 0.256 μg mL –1 ); however, this method needs much longer incubation time (180 min) for protein detection .…”

“…Presently, there are several methods that have been reported to detect these two proteins, such as chromatographic methods, − imprinting hybrid thin films or imprinting polymers, , fluorescent nanoparticles, and voltammetric immunosensor . By comparison, chromatographic analysis exhibits lower sensitivity at milligram levels, − while sensitivity information is not available for imprinting methods. , The fluorescent nanoparticles show competitive sensitivity to pepsin (DL of 0.256 μg mL –1 ); however, this method needs much longer incubation time (180 min) for protein detection . Voltammetric immunosensor presents higher sensitivity toward o-egg (DL of 0.83 pg mL –1 ) but not to pepsin.…”

Determination of pepsin by capillary electrophoresis using mixed polymer coated capillary with switchable properties toward protein adsorption/desorption

“…Hence, sensitive detection methods for pepsin are demanded in the food industry and disease detection. Several methods for the detection of pepsin have been reported, such as enzyme‐linked immunosorbent assay [11], enzymatic assay with a fluorescent substrate [13], and chromatography [14]. However, these methods have some disadvantages including a long time for analysis, complex experiments, or low detection sensitivity.…”

Scissor-based fluorescent detection of pepsin using lysozyme-stabilized Au nanoclusters

“…5 Therefore, sensitive detection methods for pepsin are required in the food industry and in disease detection. To date, several analytical techniques have been reported for pepsin assay, such as colorimetric 6 and chromatographic [7][8][9] techniques. Among these methods, the colorimetric method involves the use of a phenol reagent and suffers from poor applicability for the mixture or real samples, as well as poor selectivity toward other proteases, 10 whereas chromatographic analysis oen requires hours of work, skilled labor, and complicated procedures.…”