Affinity chromatography of rat liver amp deaminase: Evidence for single form in liver

Smith, Larry; Emerson, R.L.; Knight, G.D.

doi:10.1016/0020-711x(77)90060-x

Cited by 11 publications

(4 citation statements)

References 13 publications

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“…From the CD spectra of B-PE and b-PE obtained by Glazer et al, the curves of B-PE at low concentrations were basically consistent with those shown in Fig. 2 [9]. After (αβ) 6 of b-PE binds to one γ subunit, B-PE (αβ) 6 γ is obtained.…”

Section: Absorption Spectroscopy and Circular Dichroism Spectroscopy supporting

confidence: 82%

“…In B-PE, the γ subunit contains PUB chromophores, while the α and β subunits contain only one PEB [9,30]. Unlike PEB, PUB has extra double bonds on the A and B rings, so PUB can absorb shorter wavelength light than PEB.…”

Section: Absorption Spectroscopy and Circular Dichroism Spectroscopy mentioning

confidence: 99%

“…In 1977, Glazer obtained B-PE from Porphyridium cruentum and found that B-PE contains three subunits, the molecular weight of the α and β subunits were 19.5 kDa, and the molecular weight of the γ subunit was 29 kDa. The total molecular weight of B-PE (αβ) 6 γ was 263 kDa, and it contained Phycoerythrobilin (PEB) and Phycourobilin (PUB) chromophores [9]. In 1987, Glazer studied the amino acid structure of B-PE and found that the α subunit had 164 amino acids and the β subunit had 177 amino acids [10].…”

Section: Introductionmentioning

confidence: 99%

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Energy transfer dynamics in B-phycoerythrin from the red alga Porphyridium purpureum

Tang

et al. 2020

Chinese Journal of Physics

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The B-phycoerythrin hexamer (αβ) 6 γ of Porphyridium purpureum was isolated and purified. The absorption, circular dichroism, fluorescence and ultrafast time-resolved spectra were obtained. The results showed a double absorption peak at 545 nm and 565 nm and a shoulder peak at 498 nm, and fluorescence emission maxima at 580 nm and 620 nm were observed. The circular dichroism spectra in the near-ultraviolet region were obtained and resolved for the first time, which showed that the two peaks at 260 nm and 305 nm were considered to be correlated to phenylalanine (Phe) and tryptophan (Trp) in a conservative hydrophobic microenvironment, respectively. The circular dichroism spectra in the visible region showed that PEB139α/PEB158β and PEB82α/PEB82β existed as two exciton-coupled bilin pairs. Energy transfer within the exciton-coupled pairs was by exciton splitting, while between the exciton-coupled pairs was by Förster resonance. From the studies of the energy transfer dynamics by ultrafast time-resolved fluorescence spectroscopy, it was confirmed that the energy transfer of the B-PE hexamer had three time components of 8 ps, 60 ps, and 1200 ps. In addition, the internal energy transfer pathways of B-phycoerythrin hexamer were identified by deconvoluting the fluorescence decay curve at different detection wavelengths.

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Section: Absorption Spectroscopy and Circular Dichroism Spectroscopy supporting

confidence: 82%

Section: Absorption Spectroscopy and Circular Dichroism Spectroscopy mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Energy transfer dynamics in B-phycoerythrin from the red alga Porphyridium purpureum

Tang

et al. 2020

Chinese Journal of Physics

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“…However, the limitation of these enzymes makes it difficult for this strategy to achieve a higher level of metabolite production (Suter and Rosenbusch, 1977). Therefore, the strategy of random mutagenesis is still a desired approach that has a greater possibility of obtaining high-level production strains (Dietrich et al, 2010;Rogers et al, 2016), and biosensors have emerged as a high-throughput screening method to obtain hyperproducing strains.…”

Section: Discussionmentioning

confidence: 99%

Development of a Novel Biosensor-Driven Mutation and Selection System via in situ Growth of Corynebacterium crenatum for the Production of L-Arginine

Chen

Peng

et al. 2020

Front. Bioeng. Biotechnol.

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The high yield mutants require a high-throughput screening method to obtain them quickly. Here, we developed an L-arginine biosensor (ARG-Select) to obtain increased L-arginine producers among a large number of mutant strains. This biosensor was constructed by ArgR protein and argC promoter, and could provide the strain with the output of bacterial growth via the reporter gene sacB; strains with high Larginine production could survive in 10% sucrose screening. To extend the screening limitation of 10% sucrose, the sensitivity of ArgR protein to L-arginine was decreased. Corynebacterium crenatum SYPA5-5 and its systems pathway engineered strain Cc6 were chosen as the original strains. This biosensor was employed, and L-arginine hyperproducing mutants were screened. Finally, the HArg1 and DArg36 mutants of C. crenatum SYPA5-5 and Cc6 could produce 56.7 and 95.5 g L −1 of L-arginine, respectively, which represent increases of 35.0 and 13.5%. These results demonstrate that the transcription factor-based biosensor could be applied in high yield strains selection as an effective high-throughput screening method.

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Two forms of AMP deaminase from chicken liver

Spychała¹,

Makarewicz²

1983

Biochemical and Biophysical Research Communications

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Affinity chromatography of rat liver amp deaminase: Evidence for single form in liver

Cited by 11 publications

References 13 publications

Energy transfer dynamics in B-phycoerythrin from the red alga Porphyridium purpureum

Energy transfer dynamics in B-phycoerythrin from the red alga Porphyridium purpureum

Development of a Novel Biosensor-Driven Mutation and Selection System via in situ Growth of Corynebacterium crenatum for the Production of L-Arginine

Two forms of AMP deaminase from chicken liver

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